Optimization of Deglet-Nour Date (Phoenix dactylifera L.) Phenol Extraction Conditions
The objective of this study was to optimize the extraction conditions for phenolic compounds, total flavonoids, and antioxidant activity from Deglet-Nour variety. The extraction of active components from natural sources depends on different factors. The knowledge of the effects of different extraction parameters is useful for the optimization of the process, as well for the ability to predict the extraction yield. The effects of extraction variables, namely types of solvent (methanol, ethanol and acetone) and extraction time (1h, 6h, 12h and 24h) on phenolics extraction yield were evaluated. It has been shown that the time of extraction and types of solvent have a statistically significant influence on the extraction of phenolic compounds from Deglet-Nour variety. The optimised conditions yielded values of 80.19 ± 6.37 mg GAE/100 g FW for TPC, 2.34 ± 0.27 mg QE/100 g FW for TFC and 90.20 ± 1.29% for antioxidant activity were methanol solvent and 6 hours of time. According to the results obtained in this study, Deglet-Nour variety can be considered as a natural source of phenolic compounds with good antioxidant capacity.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1088696Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2367
 A. S. Truswell, “ABC of nutrition (4th ed.)”. London: BMJ Books, 2003.
 R. E. C. Wildman, “The nutritionist: food, nutrition and optimal health (2nd ed.)”. New York: Taylor & Francis, 2009.
 W. Vermerris, & R. Nicholson, “Phenolic compound biochemistry”. Springer: Netherlands, 2006.
 A. Bucić-Kojić, M. Planinić, S. Tomas, S. Jokić, I. Mujić, M. Bilić, & D. Velić, “Effect of Extraction Conditions on the Extractability of Phenolic Compounds from Lyophilised Fig Fruits (Ficus carica L.)”. Pol. J. Food Nutr. Sci., 61 (3), 195-199, 2011.
 R. Marija, Z. Zoran, J. Stela, & V. Senka, “Determination of optimal extraction parameters of mulberry leaves using Response Surface Methodology (RSM)”. Romanian Biotechnological Letters, 17 (3), 7295-7308, 2012.
 AOAC International, “Official methods of analysis (16th Ed.)”. Gaithersburg, MD, method 920.151, 1998.
 A. Rajaei1, M. Barzegar1, Z. Hamidi1, & M. A. Sahari, “Optimization of Extraction Conditions of Phenolic Compounds from Pistachio (Pistachia vera) Green Hull through Response Surface Method”. J. Agr. Sci. Tech., 12, 605-615, 2010.
 Y. Cai, Q. Luo, M. Sun, & H. Corke, “Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer”. Life Sciences, 74, 2157-2184, 2004.
 N. Gursoy, C. Sarikurkcu, M. Cengiz, & M. H. Solak, “Antioxidant activities, metal contents, total phenolics and flavonoids of seven Morchella species”. Food and Chemical Toxicology, 47 (9), 2381-2388, 2009.
 A. Mansouri, G. Embarek, E. Kokkalou, & P. Kefalas, “Phenolic profile and antioxidant activity of the Algerian ripe date palm fruit (Phoenix dactylifera)”. Food chemistry, 89, 411-420, 2005.
 C. Sanchez-Moreno, “Methods used to evaluate the free radical scavenging activity in foods and biological systems”. Food Science and Technology International, 8 (3), 121-137, 2002.
 M. Lu, B. Yuan, M. Zeng, & J. Chen, “Antioxidant capacity and major phenolic compounds of spices commonly consumed in China”. Food Research International, 44 (2), 530-536, 2011.
 F. Biglari, A.F.M. Alkarkhi, & A.M. Easa, “Antioxidant activity and phenolic content of various dates palm (Phoenix dactylifera) fruits from Iran”. Food Chemistry, 107, 1636-1641, 2008.
 P. K. Vayalil, “Date fruits (Phoenix dactylifera Linn): An emerging medicinal food”. Critical Reviews in Food Science and Nutrition, 52 (3), 249-271, 2012.