Cutaneous Application of Royal Jelly Inhibits Skin Lesions in NC/Nga Mice, a Human-Like Mouse Model of Atopic Dermatitis
Anti-allergic effects of royal jelly were evaluated in a human-like mouse model of atopic dermatitis. NC/Nga mice were cutaneously applied with royal jelly for 6 weeks. Royal jelly-treated mice exhibited lower levels of serum total immunoglobulin E in comparison with controls. We found that the treatment decreased (11% to the control) expression of mRNA for aquaporin-3, which is involved in the modulation of epidermal hydration. Microarray analysis revealed more than 10-fold changes in the expression of several genes, such as transglutaminase 2, repetin, and keratins. In normal human epidermal keratinocytes, royal jelly extract suppressed interleukin-8 elevation induced by TNF-α and interferon-γ, suggesting direct anti-inflammatory activity in keratinocytes. Collectively, topical application of royal jelly may be useful for amelioration of lesions and inflammation in atopic dermatitis.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1086969Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1671
 Boguniewicz M and Leung DY. “Atopic dermatitis: a disease of altered skin barrier and immune dysregulation.” Immunol Rev., 242, 2011, pp. 233-246.
 Viuda-Martos M, Ruiz-Navajas Y, Fernández-López J and Pérez-Alvarez JA. “Functional properties of honey, propolis, and royal jelly.” J. Food Sci., 73, 2008, pp. 117-124.
 Oka H, Emori Y, Kobayashi N, Hayashi Y and Nomoto K. “Suppression of allergic reactions by royal jelly in association with the restoration of macrophage function and the improvement of Th1/Th2 cell responses.” Int. Immunopharmacol., 1, 2001, pp. 521–532.
 Taniguchi Y, Kohno K, Inoue S, Koya-Miyata S, Okamoto I , Arai N, Iwaki K, Ikeda M and Kurimoto M. “Oral administration of royal jelly inhibits the development of atopic dermatitis-like skin lesions in NC/Nga mice.” Int . Immunopharmacol., 3, 2003, pp. 1313-1324.
 Tanabe S and Hochi S. “Oral administration of a galactooligosaccharide preparation inhibits development of atopic dermatitis-like skin lesions in NC/Nga mice.” Int. J. Mol. Med., 25, 2010, pp. 331-336.
 Olsson M, Broberg A, Jernås M, Carlsson L, Rudemo M, Suurküla M, Svensson PA and Benson M. “Increased expression of aquaporin 3 in atopic eczema.” Allergy., 61, 2006, pp. 1132-1137.
 Nakahigashi K, Kabashima K, Ikoma A, Verkman AS, Miyachi Y and Hara-Chikuma M. “Upregulation of aquaporin-3 is involved in keratinocyte proliferation and epidermal hyperplasia.” J. Invest. Dermatol., 131, 2011, pp. 865-873.
 Kim Y, Eom S, Kim K, Lee YS, Choe J, Hahn JH, Lee H, Kim YM, Ha KS, Ro JY and Jeoung D. “Transglutaminase II interacts with rac1, regulates production of reactive oxygen species, expression of snail, secretion of Th2 cytokines and mediates in vitro and in vivo allergic inflammation.” Mol. Immunol., 47, 2010, pp. 1010-1022.
 Huber M, Siegenthaler G, Mi rancea N, Marenholz I, Nizetic D, Breitkreutz D, Mischke D and Hohl D. “Isolation and characterization of human repetin, a member of the fused gene family of the epidermal differentiation complex.” J. Invest. Dermatol., 124, 2005, pp. 998-1007.
 Kim CH, Choi YS, Cheong K and Lee AY. “Mechanism underlying the effect of combined therapy using glucosamine and low-dose cyclosporine A on the development of atopic dermatitis-like skin lesions in NC/Nga mice.” Int. Immunopharmacol., 15, 2013, pp. 424-432.
 Theerawatanasirikul S, Sailasuta A, Thanawongnuwech R and Suriyaphol G. “Alterations of keratins, involucrin and filaggrin gene expression in canine atopic dermatitis.” Res. Vet. Sci., 93, 2012, pp. 1287-1292.