Synthesis and Characterization of PEG-Silane Functionalized Iron Oxide Nanoparticle as MRI T2 Contrast Agent

Authors: Mu-Jen Young, Cheng-Yen Wu, Wen-Yuan Hsieh

Abstract:

Iron oxide nanoparticle was synthesized by reactive-precipitation method followed by high speed centrifuge and phase transfer in order to stabilized nanoparticles in the solvent. Particle size of SPIO was 8.2 nm by SEM, and the hydraulic radius was 17.5 nm by dynamic light scattering method. Coercivity and saturated magnetism were determined by VSM (vibrating sample magnetometer), coercivity of nanoparticle was lower than 10 Hc, and the saturated magnetism was higher than 65 emu/g. Stabilized SPIO was then transferred to aqueous phase by reacted with excess amount of poly (ethylene glycol) (PEG) silane. After filtration and dialysis, the SPIO T2 contrast agent was ready to use. The hydraulic radius of final product was about 70~100 nm, the relaxation rates R2 (1/T2) measured by magnetic resonance imaging (MRI) was larger than 200(sec-1).

Keywords: Contrast Agent, Iron Oxide Nanoparticle, Magnetic Resonance Imaging, Nanoparticle Stabilization

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1086507

References:

[1] Pankhurst,Q, J Connolly, S Jones and J Dobson, “The preparation of magnetic nanoparticles for application in biomedicine” ， Journal of Physics D : Applied Physics, 2003 , 36R , p . 182
[2] Wang YX., “Superparamagnetic iron oxide based MRI contrast agents: Current status of clinical application. Quant Imaging”, Med. Surg. 2011; 1:35-40.
[3] Teja,Amyn S., Pei-YoongKoh, “Synthesis, properties, and applications of magneticiron oxide nanoparticles”, Progress in Crystal Growth and Characterization of Materials, 55 (2009) p22-45.
[4] Yi-Xiang J. Wang, Shahid M. Hussain, Gabriel P. Krestin, “Superparamagnetic iron oxide contrast agents: physicochemical characteristics and applications in MR imaging”, European Radiology, November 2001, Volume 11, Issue 11, pp 2319-2331.
[5] Chang, Wen-Hsiang, Ming-Yao Chen, Yuh-Jiuan Lin, Chin-I Lin, Shian-Jy Wang, “Method for forming superparamagnetic nanoparticles”, US 20060141149 A1, 2004.
[6] Chang, Wen-Hsiang, Wen-Uan Hsieh, Shiu-Hua Huang, Chin-I Lin, Shian-JyJassy Wang, Kelly Teng, “Magnetic nanoparticle with biocompatibility, US 20120329129 A1, 2008.
[7] Zhang, Ling, Rong He, Hong-Chen Gu, “Oleic acid coating on the monodisperse magnetite nanoparticles”, Applied Surface ScienceVolume 253, Issue 5, 30 December 2006, Pages 2611–2617.
[8] Lodhia, J., G Mandarano, NJ Ferris, P Eu, SF Cowell, “Development and use of iron oxide nanoparticles (Part 1): Synthesis of iron oxide nanoparticles for MRI”, . Biomed Imaging Interv J 2010; 6(2):e12.