Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 31172
Utilization of Laser-Ablation Based Analytical Methods for Obtaining Complete Chemical Information of Algae

Authors: Pavel Pořízka, David Prochazka, Karel Novotný, Ota Samek, ZdeněkPilát, Klára Procházková, and Jozef Kaiser


Themain goal of this article is to find efficient methods for elemental and molecular analysis of living microorganisms (algae) under defined environmental conditions and cultivation processes. The overall knowledge of chemical composition is obtained utilizing laser-based techniques, Laser- Induced Breakdown Spectroscopy (LIBS) for acquiring information about elemental composition and Raman Spectroscopy for gaining molecular information, respectively. Algal cells were suspended in liquid media and characterized using their spectra. Results obtained employing LIBS and Raman Spectroscopy techniques will help to elucidate algae biology (nutrition dynamics depending on cultivation conditions) and to identify algal strains, which have the potential for applications in metal-ion absorption (bioremediation) and biofuel industry. Moreover, bioremediation can be readily combined with production of 3rd generation biofuels. In order to use algae for efficient fuel production, the optimal cultivation parameters have to be determinedleading to high production of oil in selected cellswithout significant inhibition of the photosynthetic activity and the culture growth rate, e.g. it is necessary to distinguish conditions for algal strain containing high amount of higher unsaturated fatty acids. Measurements employing LIBS and Raman Spectroscopy were utilized in order to give information about alga Trachydiscusminutus with emphasis on the amount of the lipid content inside the algal cell and the ability of algae to withdraw nutrients from its environment and bioremediation (elemental composition), respectively. This article can serve as the reference for further efforts in describing complete chemical composition of algal samples employing laserablation techniques.

Keywords: Bioremediation, biofuels, Raman spectroscopy, Algae, laser-induced breakdown spectroscopy, Algal strains

Digital Object Identifier (DOI):

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1926


[1] M. Hannon J. Gimpel, M. Tran, B. Rasala, S. Mayfield, Biofuels from Algae: challenges and potential, Biofuels 1 (2010) 763-784.
[2] M.F. Demirbas, Biofuel from algae for sustainable development, Applied Energy 88 (2011) 3437- 3480.
[3] J.B.K. Park, R.J. Craggs, A.N. Shilton, Wastewater treatment high rate algal ponds for biofuel production, Biosource Technology 102 (2011) 35-42.
[4] A. Demirbas, Use of algae as biofuel sources, Energy Conversion and Management 51 (2010) 2738- 2749.
[5] Onlinesource., quoted 31.1.2012.
[6] X. Zeng, M.K. Danquah, X.D. Chen, Y. Lu, Microalgae bioengineering: From CO2 fixation to biofuel production, Renewable and Sustainable Energy Reviews 15 (2011) 3252-3260.
[7] L. Christenson, R. Sims, Production and harvesting of microalgae for wastewater treatment, biofuels, and biproducts, Biotechnology Advances 29 (2011) 686-702.
[8] I. Rawat, R. Ranjith Kumar, T. Mutanda, F. Bux, Dual role of microalgae: phycoremediation of domestic wastewater and biomass production for sustainable biofuels production, Applied Energy 88 (2011) 3411-3424.
[9] T.A. Davis, B. Volesky, A. Mucci, A review of the biochemisty of heavy metal biosorption by brown algae, Water Research 37 (2003) 4311-4330.
[10] M. Hoehse, D. Mory, S. Florek, F. Weritz, I. Gornushkin, U. Panne, A combined laser-induced breakdown and Raman spectroscopy Echelle system for elemental and molecular microanalysis. SpectrochimicaActa Part B 64 (2009) 1219-1227.
[11] M. Sadegh Cheri, S.H. Tavassoli, Quantitative analysis of toxic metals lead and cadmium in water jet by laser-induced breakdown spectroscopy, Applied optics 50 (2011) 1227-1233.
[12] P. Porizka, D. Prochazka, J. Novotny, R. Malina, J. Kaiser, O. Samek, L. Krajcarova, Measurements of algal strain using different LIBS setups. SpectrochimicaActa 69 (2012) 613-619.
[13] S. Ramya, R.P. George, R.V. SubbaRao, R.K. Dayal, Detection of algae and bacterial biofilms formed on titanium surfaces using micro-Raman analysis, Applied Surface Science 256 (2010) 5108-5115.
[14] O. Samek, A. Jon├í┼í, Z. Pil├ít, P. Zem├ínek, L. Nedbal, J. Tř├¡ska, P. Kotas, M. Trt├¡lek, Raman Microspectroscopy of Individual Algal Cells: Sensing Unsaturation of Storage Lipids in vivo. Sensors 10 (2010) 8635-8651.
[15] H. Wu, J.V. Volponi, A.E. Oliver, A.N. Parikh, B.A.Simmons, S. Singh, In vivo lipidomics using single-cell Raman spectroscopy, PNAS 108 (2011) 3809-3814.
[16] T. ┼ÿezanka, M. Petr├ínkov├í. V. Cep├ík, P. Přibyl, K. Sigler, T. Cajthmal, Trachydiscusminutus, a New Biotechnological Source of Eicosapentaenoic acid. Folia Microbiol. 55 (3) 265-269.
[17] P. Greenspan, E.P. Mayer, S.D. Fowler, Nile red: A selective fluorescent stain for intracellular lipid droplets. J. Microbiol. Meth. 68 (2007) 639-642.
[18] B. Ham, R. Shelton, B. Butler, P. Thionville: Calculating the iodine value for marine oils fatty acid profiles, J. Am. Oil. Chem. Soc. 75 (2008) 4717-4722.