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Fermentative Production and Characterization of Carboxymethyl Bacterial Cellulose Using Date Syrup

Authors: Marzieh Moosavi-Nasab, Ali R. Yousefi, Hamed Askari, Maryam Bakhtiyari

Abstract:

In this study, static batch fermentation was used for bacterial cellulose production in date syrup solution (Bx. 10%) at 28°C using Gluconacetobacter. xylinus (PTCC 1734). The physicochemical properties of standard Sigma CMC and the produced carboxymethyl bacterial cellulose (CMBC) were studied using FT-IR spectroscopy, X-ray diffractometry (XRD) and Scanning Electron Microscopy (SEM). According to the FT-IR spectra the bands at 1664 and 1431 cm-1 indicate that carboxylic acid groups and carboxylate groups exist on the surface. The SEM imaging of CMBC and CMC carried out in magnification of 1K. Comparing the SEM imaging obviously showed that the ribbon shape in CMC remained but the length of ribbons became shorter while that shape changed to flake shape for CMBC. Determination of the area under XRD patterns demonstrated that the crystallinity amount of CMC was more than that for CMBC (51.08% and 81.84% for CMBC and CMC, respectively).

Keywords: Carboxymethyl bacterial cellulose, Fourier Transform Infrared spectroscopy, Scanning Electron Microscopy, X-ray diffractometry.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1085239

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References:


[1] R. M. Brown, "Microbial cellulose as a building block resource for specialty products and processes therefore, " PCT Int. Appl. WO 8912107 A1, 37, 1989.
[2] D. Charpentier, G. Mocanu, A. Carpov, S. Chapelle, L. Merle and G. Muller, "New hydrophobically modified carboxymethyl cellulose derivatives, " Carbohydrate Polymer, vol. 33, pp. 177-186, 1997.
[3] H. N. Cheng, M. Takai and E. A. Ekong, "Rheology of carboxymethylcellulose made from bacterial cellulose, " Macromolecular Symposia, vol. 140, pp. 145-153,1999.
[4] O. R. Fennema, "Food Chemistry, " 3rd Edition, ISBN 0-8247-9346-3, pp. 203-210, 1997.
[5] R. Jonasand L. F. Farah, "Production and application of microbial cellulose, " Polymer Degradation and Stability, vol. 59, pp. 101-106, 1998.
[6] W. M. Kulicke, A. H. Kull, W. Kull, H. Thielking, J. Engelhardt and J. B. Pannek, "Characterization of aqueous carboxymethylcellulose solutions in terms of their molecular structure and its influence on rheological behaviour, " Polymer, vol. 37, pp. 2723-2731, 1996.
[7] B. T. Miranda, S. R. Miranda, L. P. Chan and E. R. Saqueton, "Some studies on nata, " Nat. Appl. Sci. Bull. (Univ. Philippines), vol. 19, pp. 67-79, 1965.
[8] M. Nogi, K. Handa, A. N. Nakagaito and H. Yano, "Optically transparent bionanofiber composites with low sensitivity to refractive index of the polymer matrix, " Applied Physics Letters, vol. 87, pp. 1-3, 2005.
[9] J. Shah and R. M. Brown, "Towards electronic paper displays made from microbial cellulose, " Applied Microbiology and Biotechnology, vol. 66, pp. 352-355, 2005.
[10] A. M. Sokolnicki, R. J. Fisher, T. P. Harrahand, D. L. Kaplan, "Permeability of bacterial cellulose membranes. Journal of Membrane Science, " vol. 272, pp.15-27, 2006.
[11] K. Watanabe, Y. Eto, S. Takano, S. Nakamori, H. Shibai and S. Yamanaka, "A new bacterial cellulose substrate for mammalian cell culture. Cytotechnology, " vol. 13, 1993.