Alignment of MG-63 Osteoblasts on Fibronectin-Coated Phosphorous Doping Lattices in Silicon

Authors: Andreas Körtge, Susanne Stählke, Regina Lange, Mario Birkholz, Mirko Fraschke, Katrin Schulz, Barbara Nebe, Patrick Elter

Abstract:

A major challenge in biomaterials research is the regulation of protein adsorption which is a key factor for controlling the subsequent cell adhesion at implant surfaces. The aim of the present study was to control the adsorption of fibronectin (FN) and the attachment of MG-63 osteoblasts with an electronic nanostructure. Shallow doping line lattices with a period of 260 nm were produced for this purpose by implantation of phosphorous in silicon wafers. Protein coverage was determined after incubating the substrate with FN by means of an immunostaining procedure and the measurement of the fluorescence intensity with a TECAN analyzer. We observed an increased amount of adsorbed FN on the nanostructure compared to control substrates. MG-63 osteoblasts were cultivated for 24h on FN-incubated substrates and their morphology was assessed by SEM. Preferred orientation and elongation of the cells in direction of the doping lattice lines was observed on FN-coated nanostructures.

Keywords: Cell adhesion, electronic nanostructures, doping lattice, fibronectin, MG-63 osteoblasts, protein adsorption.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1082829

References:

[1] C.J. Bettinger, R. Langer, J.T. Borenstein, Engineering Substrate Topography at the Micro- and Nanoscale to Control Cell Function, Angew. Chem. Int. Ed. 48 (2009) 5406-5415.
[2] C. Galli, M.C. Coen, R. Hauert, V.L. Katanaev, M.P. Wymann, P. Gröning, L. Schlapbach, Protein adsorption on topographically nanostructured titanium, Surface Science 474 (2001) L180-L184.
[3] P. Roach, D. Farrar, C.C. Perry, Surface Tailoring for Controlled Protein Adsorption: Effect of Topography at the Nanometer Scale and Chemistry, J. Am. Chem. Soc. 128 (2006) 3939-3945.
[4] P. Elter, R. Lange, U. Beck, Atomic force microscopy studies of the influence of convex and concave nanostructures on the adsorption of fibronectin, Colloids and surfaces 89 (2012) 139-146.
[5] G.B. Sigal, M. Mrksich, G.M. Whitesides, Effect of Surface Wettability on the Adsorption of Proteins and Detergents, J. Am. Chem. Soc. 120 (1998) 3464-3473.
[6] Y. Arima, H. Iwata, Effect of wettability and surface functional groups on protein adsorption and cell adhesion using well-defined mixed selfassembled monolayers, Biomaterials 28 (2007) 3074-3082.
[7] M. Birkholz, P. Zaumseil, J. Bauer, D. Bolze, G. Weidner, Small-angle reciprocal space mapping of surface relief gratings, Materials science & engineering 27 (2007) 1154-1157.
[8] D. Knoll, K. Ehwald, B. Heinemann, A. Fox, K. Blum, H. Rucker, F. Furnhammer, B. Senapati, R. Barth, U. Haak, W. Hoppner, J. Drews, R. Kurps, S. Marschmeyer, H. Richter, T. Grabolla, B. Kuck, O. Fursenko, P. Schley, R. Scholz, B. Tillack, Y. Yamamoto, K. Kopke, H. Wulf, D. Wolansky, W. Winkler, A flexible, low-cost, high performance SiGe:C BiCMOS process with a one-mask HBT module, pp. 783-786.
[9] D. Knoll, B. Heinemann, R. Barth, K. Blum, J. Borngraber, J. Drews, K.-E. Ehwald, G. Fischer, A. Fox, T. Grabolla, U. Haak, W. Hoppner, F. Korndorfer, B. Kuck, S. Marschmeyer, H. Richter, H. Rucker, P. Schley, D. Schmidt, R. Scholz, B. Senapati, B. Tillack, W. Winkler, D. Wolansky, C. Wolf, H.-E. Wulf, Y. Yamamoto, P. Zaumseil, A modular, low-cost SiGe:C BiCMOS process featuring high-f/sub T/ and high BV/sub CEO/ transistors, pp. 241-244.
[10] F. Grinnell, M.K. Feld, Fibronectin adsorption on hydrophilic and hydrophobic surfaces detected by antibody binding and analyzed during cell adhesion in serum-containing medium, J. Biol. Chem.257 (1982) 4888-4893.