@article{(Open Science Index):https://publications.waset.org/pdf/14358, title = {Alignment of MG-63 Osteoblasts on Fibronectin-Coated Phosphorous Doping Lattices in Silicon}, author = {Andreas Körtge and Susanne Stählke and Regina Lange and Mario Birkholz and Mirko Fraschke and Katrin Schulz and Barbara Nebe and Patrick Elter}, country = {}, institution = {}, abstract = {A major challenge in biomaterials research is the regulation of protein adsorption which is a key factor for controlling the subsequent cell adhesion at implant surfaces. The aim of the present study was to control the adsorption of fibronectin (FN) and the attachment of MG-63 osteoblasts with an electronic nanostructure. Shallow doping line lattices with a period of 260 nm were produced for this purpose by implantation of phosphorous in silicon wafers. Protein coverage was determined after incubating the substrate with FN by means of an immunostaining procedure and the measurement of the fluorescence intensity with a TECAN analyzer. We observed an increased amount of adsorbed FN on the nanostructure compared to control substrates. MG-63 osteoblasts were cultivated for 24h on FN-incubated substrates and their morphology was assessed by SEM. Preferred orientation and elongation of the cells in direction of the doping lattice lines was observed on FN-coated nanostructures.}, journal = {International Journal of Chemical and Molecular Engineering}, volume = {7}, number = {1}, year = {2013}, pages = {70 - 73}, ee = {https://publications.waset.org/pdf/14358}, url = {https://publications.waset.org/vol/73}, bibsource = {https://publications.waset.org/}, issn = {eISSN: 1307-6892}, publisher = {World Academy of Science, Engineering and Technology}, index = {Open Science Index 73, 2013}, }