Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 30850
Proteomic Analysis of Tumor Tissue after Treatment with Ascorbic Acid

Authors: Seyeon Park, Mi Jang


Tumor cells have an invasive and metastatic phenotype that is the main cause of death for cancer patients. Tumor establishment and penetration consists of a series of complex processes involving multiple changes in gene expression. In this study, intraperitoneal administration of a high concentration of ascorbic acid inhibited tumor establishment and decreased tumor mass in BALB/C mice implanted with S-180 sarcoma cancer cells. To identify proteins involved in the ascorbic acid-mediated inhibition of tumor progression, changes in the tumor proteome associated with ascorbic acid treatment of BALB/C mice implanted with S-180 were investigated using two-dimensional gel electrophoresis and mass spectrometry. Twenty protein spots were identified whose expression was different between control and ascorbic acid treatment groups.

Keywords: Proteomic Analysis, ascorbic acid, S-180 implantedBALB/C mouse

Digital Object Identifier (DOI):

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1376


[1] S. J. Padayatty, H. Sun, Y. Wang, H. D. Riordan, S. M. Hewitt, A. Katz, R. A. Wesley, and M. Levine, "Vitamin C pharmacokinetics: implications for oral and intravenous use," Ann Intern Med, vol. 140, pp. 533-537, 2004.
[2] S. Bram, P. Froussard, M. Guichard, C. Jasmin, Y. Augery, F. Sinoussi-Barre, and W. Wray, "Ascorbic acid preferential toxicity for malignant melanoma cells," Nature vol. 284, pp. 629-631, 1980.
[3] G. Bruchelt, L. Baader, A. G. Reith, N. L. Holger, S. Gebhardt, and D. Niethammer, "Rationale for the use of ascorbic acid in neuroblastoma therapy," Human Neuroblastoma. Newark: Harwood Academic Publishers, pp. 34-40, 1993.
[4] S. Fujinaga, H. Sakagami, N. Kuribayashi, H. Takahashi, Y. Amano, T. Sakagami, and M. Takeda, "Possible role of hydrogen peroxide in apoptosis induction by ascorbic acid in human myelogenous leukemic cell lines," Showa Univ Med Sci, vol. 6, pp. 135-144, 1994.
[5] V. De Laurenzi, G. Melino, I. Savini, M. Annicchiarico-Petruzzelli, A. Finazzi-Agro, and L. Avigliano, "Cell death by oxidative stress and ascorbic acid regeneration in human neuroectodermal cell lines," Eur J Cancer, vol 31A, pp. 463-466, 1995.
[6] C. H. Park, B. F. Kimler, D. Bodensteiner, S. R. Lynch, and R. S. Hassanein, "In vitro growth modulation by L-ascorbic acid of colony-forming cells from bone marrow of patients with myelodysplastic syndromes," Cancer Res, vol. 52, pp. 4458-4466, 1992.
[7] C. H. Park, "Biological nature of the effect of ascorbic acids on the growth of human leukemic cells," Cancer Res, vol. 45, pp. 3969-3973, 1985.
[8] S. Park, S. S. Han, C. H. Park, E. R. Hahm, S. J. Lee, H. K. Park, S. H. Lee, W. S. Kim, C. W. Jung, K. Park, H. D. Riordan, B. F. Kimler, K. Kim, and J. H. Lee, "L-Ascorbic acid induces apoptosis in acute myeloid leukemia cells via hydrogen peroxidemediated mechanisms," Int J Biochem Cell Biol, vol. 36, pp. 2180-2195, 2004.
[9] I. J. Fidler, "Critical determinants of cancer metastasis: rationale for therapy," Cancer Chemother Pharmacol, vol. 43, pp. S3-10, 1999.
[10] P. Mignatti and D. B. Rifkin, "Biology and biochemistry of proteinases in tumor invasion," Physiol Rev, vol. 73, pp. 161-195, 1993.
[11] M. Rath and L. Pauling, "Plasmin-induced proteolysis and the role of apoprotein(a), lysine and synthetic analogs," Orthomol Med, vol. 7, pp. 17-23, 1992.
[12] M. W. Roomi, N. W. Roomi, V. Ivanov, T. Kalinovsky, A. Niedzwiecki, and M. Rath, "Modulation of N-methyl-N-nitrosourea induced mammary tumors in Sprague-Dawley rats by combination of lysine, proline, arginine, ascorbic acid and green tea extract," Breast Cancer Res, vol. 7, pp. R291-295, 2005.
[13] E. C. Kohn, "Development and prevention of metastasis," Anticancer Res, vol. 13, pp. 2553-2559, 1993.
[14] E. Wybieralska, M. Koza, J. Sroka, J. Czyz, and Z. Madeja, "Ascorbic acid inhibits the migration of Walker 256 carcinosarcoma cells," Cell Mol Biol Lett, vol. 13, pp. 103-111, 2008.
[15] G. G. Meadows, H. F. Pierson, and R. M. Abdallah, "Ascorbate in the treatment of experimental transplanted melanoma," Am J Clin Nutr, vol. 54, pp. 1284S-1291S, 1991.
[16] H. S. Taper, J. M. Jamison, J. Gilloteaux, J. L. Summers, and P. B. Calderon, "Inhibition of the development of metastases by dietary vitamin C:K3 combination," Life Sci, vol. 75, pp. 955-967, 2004.