Commenced in January 2007
Paper Count: 30855
Determination of Effective Variables on Arachidonic Acid Production by Mortierella alpina CBS 754.68in Solid-State Fermentation using Plackett-Burman Screening Design
Abstract:In the present study, the oleaginous fungus Mortierella alpina CBS 754.68 was screened for arachidonic acidproduction using inexpensive agricultural by-products as substrate. Four oilcakes were analysed to choose the best substrate among them. Sunflower oilcake was the most effective substrate for ARA production followed by soybean, colza and olive oilcakes. In the next step, seven variables including substrate particle size, moisture content, time, temperature, yeast extract supply, glucose supply and glutamate supply were surveyed and effective variables for ARA production were determined using a Plackett-Burman screening design. Analysis results showed that time (12 days), substrate particle size (1-1.4 mm) and temperature (20ºC) were the most effective variables for the highest level of ARA production respectively.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1079822Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1902
 H. D. Jang, S. S. Yang. Polyunsaturated fatty acids production with a solid-state column reactor. Bioresource Technol. 2008, 99: 6181-6189.
 M. H. Cheng, T. H. Walker, G. J. Hulbert and D. R. Raman. Fungal production of eicosapentaenoic acid and arachidonic acids from industrial waste streams and crude soybean oil. Bioresource Technol. 1999, 67: 101-110.
 S. Papanilolaou, M. Komaitis and G. Aggelis. Single cell oil (SCO) production by Mortierella isabellina grown on high-sugar content media. Bioresource Technol. 2004, 95: 287-291
 T. Aki, Y. Nagahata, K. Ishihara, Y. Tanaka, T. Morinaha, K. Higashiyama, K. Akimoto, S. Fujikawa, S. Kawamoto, S. Shigeta, K. ono and O. Suzuki. Production of arachidonic acid by filamentous fangus, Mortierella alliacea strain YN-15. J Amer Oil Chem Soc. 2001, 78(6): 599-604
 H. D. Jang, Y. Y. Lin and S. S. Yang. Polyunsaturated fatty acid production with Mortierella alpina by solid substrate fermentation. Bot Bull Acad Sin. 2000, 41: 41-48.
 M. Certik, L. Slavikova and J. Sajbidor. Enhancement of nutritional value of cereals with ╬│-linolenic acid by fungal solid state fermentation. Food Technol Biotechnol. 2006, 44(1): 75-82
 E. Conti, M. Stredansky, S. Stredanska and F. Zanetti. ╬│-Linolenic acid production by solid-state fermentation of Mucorales strains on cereals. Bioresourse Technol. 2001, 76: 283-286.
 H. Gema, A. Cavadia, D. Dimou, V. Tsagou, M. Komaitis and G. Aggelis. Production of ╬│-linolenic acid by Cunninghamella echinulata cultivated on glucose and orange peel. Appl Microbiol Biotechnol. 2002, 58: 303-307
 P. Jangbua, K. Laoteng, P. Kitsubun, M. Nopharatana and A. Tongata. Gamma-linolenic acid production of Mucor rouxii by solid-state fermentation using agricultural by-products. Lett Appl Microbiol. 2009, 49(1): 91-97
 M. Veen, C. lang. Production of lipid compounds in the yeast Saccharomyces servisiae. Appl Microbiol Biotechnol. 2004, 63: 635-646
 M. Zhu, L.-J. Yu and Y.-X. Wu. An inexpensive medium for production of arachidonic acid by Mortierella alpina. J Ind Microbiol Biotechnol. 2003, 30: 75-79.
 P. B. L. Fregolente, L. V. Fregolente, M. R. W. Maciel and P. O. Carvalho. Screening of microbial lipases and evalutaion of their potential to produce glycerides with high gamma linolenic acid concentration. Braz J Microbiol. 2009, 40(4): 747-756
 M. Stradansky, E. Conti and A. Salaris. Production of polyunsaturated fatty acids by Pythium ultimum in solid-stste cultivation. Enzyme Microb Technol. 2000, 26: 304-307
 L. C. Metcalf, A. A. Schmitz, and J. R. Pelka. Rapid preparation of methyl esters from lipid for gas chromatography analysis. Anal Chem. 1996, 38: 514-518.
 S. Stredanska, D. Slugen, M. Stredansky and J. Grego. Arachidonic acid production by Mortierella alpina grown on solid substrates. World J Microbiol Biotechnol. 1993, 9: 511-513.