%0 Journal Article
	%A Z. Ghobadi and  Z. Hamidi- Esfahani and  M. H. Azizi
	%D 2011
	%J International Journal of Chemical and Molecular Engineering
	%B World Academy of Science, Engineering and Technology
	%I Open Science Index 57, 2011
	%T Determination of Effective Variables on Arachidonic Acid Production by Mortierella alpina CBS 754.68in Solid-State Fermentation using Plackett-Burman Screening Design
	%U https://publications.waset.org/pdf/12766
	%V 57
	%X In the present study, the oleaginous fungus
Mortierella alpina CBS 754.68 was screened for arachidonic
acidproduction using inexpensive agricultural by-products as
substrate. Four oilcakes were analysed to choose the best substrate
among them. Sunflower oilcake was the most effective substrate for
ARA production followed by soybean, colza and olive oilcakes. In
the next step, seven variables including substrate particle size,
moisture content, time, temperature, yeast extract supply, glucose
supply and glutamate supply were surveyed and effective variables
for ARA production were determined using a Plackett-Burman
screening design. Analysis results showed that time (12 days),
substrate particle size (1-1.4 mm) and temperature (20ÂșC) were the
most effective variables for the highest level of ARA production
respectively.
	%P 800 - 802