Commenced in January 2007
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Effect of Process Parameters on Aerobic Decolourization of Reactive Azo Dye using Mixed Culture

Authors: Kapil Kumar, M. G. Dastidar, T. R. Sreekrishnan

Abstract:

In the present study, an attempt was made to examine the potential of aerobic mixed culture for decolourization of Remazol Black B dye in batch reactors. The effect of pH, temperature, inoculum, initial concentration of dye and initial concentration of glucose was studied with an aim to determine the optimal conditions required for maximum decolourization and degradation. The culture exhibited maximum decolourization ability at pH between 7-8 and at 30°C. A 10% (v/v) inoculum and 1% (w/v) glucose concentration were found to be the optimum for decolourization. A maximum of 98% decolourization was observed at 25 ppm initial concentration of dye after 18 hours of incubation period. At higher dye concentration of 300 ppm, the removal in colour was found to be 75% in 48 hours of incubation period. The results show that the enriched mixed culture from activated sludge has good potential in removal of Remazol Black B dye from wastewater under aerobic conditions.

Keywords: Aerobic conditions, Decolourization, Mixed culture, Remazol Black B.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1079428

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References:


[1] Banat, IM.; Nigam , P.; Singh, D.; Marchant, R. Microbial decolorization of textile-dye containing effluents - a review. Bioresource Technology. 1996 (58) 217-227.
[2] Wang, W. Toxicity assessment of pretreated industrial effluent using higher plant. Research Journal Water Pollution Control Federation. 1991, (62) 853- 860.
[3] Kapustka, L.A.; Reporter, M. Terrestrial primary producers, in: P. Calow (Ed.), Handbook of Ecotoxicology, vol. 1, Blackwell Scientific Publications, Oxford, 1993, 278-297.
[4] Kalyuzhnyi, S.; Sklyar, V. Biomineralisation of azo dyes and their breakdown products in anaerobic-aerobic hybrid and UASB reactors. Water Science Technology. 2000, ( 41) 23- 30.
[5] Kariminiaae, Hamedaani.; Sakurai, A.; Sakakibara, M.; Decolorization of synthetic dyes by a new manganese peroxidase-producing white rot fungus. Dyes Pigments. 2007 (72) 157-162.
[6] Asad, S.; Amoozegar,M.A,; Pourbabaee, A.A.; Sarbolouki, M.N.; Dastgheib, S.M.M. Decolorization of textile azo dyes by newly isolated halophilic and halotolerant bacteria. Bioresource Technology. 2007, 2082- 2088.
[7] Moosvi,S.;Kher,X.;Madamwar, D. Isolation, characterization and decolorization of textile dyes by amixed bacterial consortium JW-2. Dyes Pigments. 2007 (74) 723-729.
[8] Aksu, Z.; Tezer, S. Equilibrium and kinetic modelling of biosorption of Remazol Black B by Rhizopus arrhizus in a batch system: effect of Temperature. Process Biochemistry.2000 (36) 431-439.
[9] Kirby, N.; Marchant, R.; McMullan, G. Decolourisation of synthetic textile dyes by Phlebia tremellosa. FEMS Microbiology Letters. 2000 (188) 93-96.
[10] Swamy, J.; Ramsay, J.A.The evaluation of white rot fungi in the decolouration of textile dyes. Enz Microbial Technology.1999 (24) 130-137.
[11] Aksu, Z.; Donmez, G. Combined effects of molasses sucrose and reactive dye on the growth and dye bioaccumulation properties of Candida tropicalis. Process Biochemistry. 2005 (40) 2443-2454.
[12] Meehan, C.; Banat, IM.; McMullan, G.; Nigam, P.; Smyth, F.; Marchant, R. Decolorization of Remazol Black-B using a thermotolerant yeast, Kluyveromyces marxianus IMB3. Environment Internatioanl. 2000. (1-2) 75- 79.
[13] Robinson, T.; Chandran, B.; Nigam, P. Effect of pretreatments of three waste resiudes, wheat straw, corncobs and barley husks on dye adsorption. Bioresour Technol. 2002 (85) 119-124.
[14] Hepel,M.; Hazelton,S. Photoelectrochemical Degradation of Diazo Dyes on Nanostructured Electrodes Electrochim Acta. 2005 (50) 5278-5291.
[15] Vinodgopal, K.; Peller, J.; Makogon, O.; Kamat, P.V. Ultrasonic mineralization of a reactive textile azo dye, Remazol Black B. Water Research. 1998(32) 3646.
[16] Pearce, CI.; Lloyd, JR.; Guthrie, JT. The removal of colour from textile wastewater using whole bacterial cells: a review. Dyes and Pigment. 2003 (58),179-196.
[17] Andre, BDS.; Francisco, JC.; Jules, BVL. Review paper on current technologies for decolourisation of textile wastewaters: perspectives for anaerobic biotechnology. Bioresource Technology. 2007 (98) 2369- 2385.
[18] Chang, Jo-Shu.; Chen, Bor-Yann.; Lin, YS. Stimulation of bacterial decolorization of an azo dye by extracellular metabolites from Escherichia coli strain NO3. Bioresource Technology. 2004 (91) 243-48.
[19] Chen, K.C.; Huang, W.T.; Wu, J.Y.; Houng, J.Y. Microbial decolorization of azo dyes by Proteus mirabilis, Journal of Industrial Microbiology and Biotechnology. 1999 (23) 686-690.
[20] Aksu, Z. Reactive dye bioaccumulation by Saccharomyces cerevisiae. Process Biochem. 2003(10) 1437-1444.
[21] Cetin, D.; Donmez,G. Decolorization of reactive dyes by mixed cultures isolated from textile effluent under anaerobic conditions. Enzyme and Microbial Technology. 2006 (38) 926-930.