Bioremediation of Oil-Polluted Soil of Western Kazakhstan

Authors: S. A. Aytkeldiyeva, A. K. Sadanov, E. R. Faizulina, A. A. Kurmanbayev

Abstract:

15 strains of oil-destructing microorganisms were isolated from oil polluted soil of Western Kazakhstan. Strains 2-A and 41-3 with the highest oil-destructing activities were chosen from them. It was shown that these strains oxidized n-alkanes very well, but isoalkanes, isoparaffin, cycloparaffin and heavy aromatic compounds were destructed very slowly. These both strains were tested as preparations for bioremediation of oil-polluted soil in model and field experiments. The degree of utilizing of soil oil by this preparation was 79-84 % in field experiments.

Keywords: Bioremediation, n-alkanes, oil-polluted soil, oiloxidizingmicroorganisms.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1079062

References:

[1] B.G. Khayirov, "Modern ecological problems in oil branch of the Republic of Kazakhstan", Neft and gas Journ., no. 3, pp. 93-98, 2001 (In Russian)
[2] T.P. Sserikov, R.R.Sagyndykova, V.ð£.Yugai, A.B.Yeskuzhieva, "About the preservation of the environment in conditions of oil and gas producton on enterprises open joint-stock company «Kazakhoil-Emba»", Neft and gas Journ., no. 1, pp.83-87, 2001. (In Russian)
[3] N.A. Kireeva, M.D.Bakaeva, N.F.Galimzyanova "Evaluation of the effect of Various Methods of Oil-polluted soil bioremediation on micromycete complexes", Appl. Biochem. Microbiol. no. 1, pp.63-68, 2008. (in Russian)
[4] N.N. Pozdnykova, V.E. Nikitina, O.V. Turovskaya, "Bioremediation of Oil-polluted soil with an Association including the fungus Pleurotus ostreatus and soil microflora", Appl. Biochem. Microbiol. v ol. 44, no. 1. pp. 60-65, 2008. (In Russian)
[5] "Method of determination of extracted oil carbohydrates - Massachusetts Department of Environmental protection", MADEP-EPH- 98-1
[6] "Method of determination of oil carbohydrates - Florida Department of Environmental protection", Method #FL-PRO
[7] L.O.Odokuma, M.N. Inor, "Nitrogen fixing bacteria enhanced bioremediation of a crude oil polluted soil", Global J. of Pure and Appl., vol. 8, no. 4, pp. 455-470, 2002.