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Contamination of Organochlorine Pesticides in Nest Soil, Egg, and Blood of the Snail-eating Turtle (Malayemys macrocephala) from the Chao Phraya River Basin, Thailand

Authors: Sarun Keithmaleesatti, Pakorn Varanusupakul, Wattasit Siriwong, Kumthorn Thirakhupt, Mark Robson, Noppadon Kitana

Abstract:

Organochlorine pesticides (OCPs) are known to be persistent and bioaccumulative toxicants that may cause reproductive impairments in wildlife as well as human. The current study uses the snail-eating turtle Malayemys macrocephala, a long-lived animal commonly distribute in rice field habitat in central part of Thailand, as a sentinel to monitor OCP contamination in environment. The nest soil, complete clutch of eggs, and blood of the turtle were collected from agricultural areas in the Chao Phraya River Basin, Thailand during the nesting season of 2007-2008. The novel methods for tissue extraction by an accelerated solvent extractor (ASE, for egg) and liquid-liquid extraction (for blood) have been developed. The nineteen OCP residues were analyzed by gas chromatography with micro-electron captured detector (GC-μECD). The validated methods have met requirements of the AOAC standard. The results indicated that significant amounts of OCPs are still contaminated in nest soil and eggs of the turtle even though the OCPs had been banned in this area for many years. This suggested the potential risk to health of wildlife as well as human in the area.

Keywords: Gas chromatography, persistent organic pollutants, rice field, sentinel species.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1078633

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References:


[1] A. S. Perry, I. Yamamato, I. Ishaaya and R. Perry, Insecticides in Agriculture and Environment: Retrospects and Prospects, Berlin: Springer, 1998.
[2] C. H. Walker, S. P. Hopkin, R. M. Sibly and D. B Peakall, Principles of Ecotoxicology. 2nd ed, Boca Raton, FL: Taylor & Francis, 2001.
[3] W. P. Cunningham, M. A. Cunningham and S. B. Woodworth, Environmental Science: A Global Concern, 9th ed., New York: McGraw- Hill, 2007.
[4] K. Thirakhupt, D. Sitthicharoenchai, S. Keithmaleesatti and W. Siriwong, "Organochlorine pesticides and their usages in Thailand: A review," J. Sci. Res. Chulalongkorn Univ., vol. 31, pp. 1-15, 2006.
[5] T. Damstra, S. Barlow, A. Bergman, R. Kavlock, and G. Van Der Kraak, Global Assessment of the State-of-the-Science of Endocrine Disruptors. Geneva: International Programme on Chemical Safety, 2002.
[6] L. J. Guillette, T. S. Gross, G. R. Masson, J. M. Matter, H. F. Percival and A. R. Woodward, "Developmental abnormalities of the gonad and abnormal sex hormone concentrations in juvenile alligators from contaminated and control lakes in Florida," Environ. Health Perspect., vol. 102, pp. 680-688, 1994.
[7] L. J. Guillette, D. B. Pickford, D. A. Crain, A. A. Rooney and H. F. Percival, "Reduction in penis size and plasma testosterone concentrations in juvenile alligators living in a contaminated environment," Gen. Comp. Endocrinol., vol.101, pp. 32-42, 1996.
[8] W. A. Hopkins, "Use of tissue residues in reptile ecotoxicology: A call for integration and experimentation" in Toxicology of Reptiles, S. C. Gardner and E. Oberdörster, Eds. Boca Raton, FL: Taylor & Francis, 2006.
[9] J. M. Keller, J. R. Kucklick and P. D. McClellan-Green, "Organochlorine contaminants in Loggerhead sea turtle blood: extraction techniques and distribution among plasma and red blood cells," Arch. Environ. Contam. Toxicol. vol. 46, pp. 254-264, 2004.
[10] J. J. Alava, J. M. Keller, J. R. Kucklick, J. Wyneken, L. Crowder and G. I. Scott, "Loggerhead sea turtle (Caretta caretta) egg yolk concentrations of persistent organic pollutants and lipid increase during the last stage of embryonic development," Sci. Total Environ., vol. 367, pp. 170-181, 2006.
[11] C. A. Bishop, P. Ng, K. E. Pettit, S. W. Kennedy, J. J. Stegeman, R. J. Norstrom and R. J. Brooks, "Environmental contamination and developmental abnormalities in eggs and hatchlings of the common snapping turtle (Chelydra serpentina serpentina) from the Great Lakes- St. Lawrence River (1989 - 1991)," Environ. Pollut., vol. 101, pp. 143- 156, 1998.
[12] S. R. de Solla, K. J. Fernie and S. Ashpole, "Snapping turtles (Chelydra serpentina) as bioindicators in Canadian Areas of concern in the Great Lakes Basin II. Changes in hatching success and hatchling deformities in relation to persistent organic pollutants," Environ. Pollut., vol. 153, pp. 529-536, 2008.
[13] C. A. Bishop, D. R. S. Lean, R. J. Brooks, J. H. Carey and P. Ng, "Chlorinated hydrocarbons in early life stages of the common snapping turtle (Chelydra serpentina serpentina) from a coastal wetland on Lake Ontario," Environ. Toxicol. Chem., vol. 14, pp. 421-426, 1995.
[14] W. Siriwong, "Organochlorine pesticide residues in aquatic ecosystem and health risk assessment of local agricultural community," Ph.D. dissertation, Graduate School, Chulalongkorn Univ., Bangkok, Thailand, 2006.
[15] S. Keithmaleesatti, K. Thirakhupt, A. Pradatsudarasar, P. Varanusupakul, N Kitana, and M. Robson, "Concentration of organochlorine in egg yolk and reproductive success of Egretta garzetta (Linnaeus, 1758) at Wat Tan-en Non-hunting Area, Phra Nakhorn Si Ayuthaya Province, Thailand," Ecotox. Environ. Safe., vol. 68, pp. 79- 83, 2007.
[16] N. Srinarumol, "Population biology of the Malayan snail-eating turtle Malayemys subtrijuga (Schlegel and Muller, 1844)," M.S. thesis, Dept. Biology, Fac. Sci., Chulalongkorn Univ., Bangkok, Thailand, 1995.
[17] T. R. Brophy, "Geographic variation and systematics in the South-east Asia turtle of the genus Malayemys (Testudines: Bataguridae)," Hamadryad, vol. 29, pp. 63-79, 2004.
[18] F. Bonin, B. Devaux and A. Dupré, Turtles of the World, 1st ed., Baltimore, MD: The Johns Hopkins University Press, 2006.
[19] W. Siriwong, K. Thirakhupt, D. Sitticharoenchai and M. Robson, "Accumulation of organochlorine pesticide residues in aquatic plants," J. Sci. Res. Chulalongkorn Univ., vol. 32, pp.7-14, 2007.
[20] R. D. Pridmore, S. F. Thrush, V. J. Cummings and J. E. Hewitt, "Effect of the organochlorine pesticide technical chlordane on intertidal macrofauna," Mar. Pollut. Bull., vol. 24, pp. 98-102, 1992.
[21] B. Pan, W. X. Liu, Z. Shi, J. Cao, W. R. Shen, B. P. Qing, R. Sun and S. Tao, "Sample purification for analysis of organochlorine pesticides in sediment and fish muscle," J. Environ. Sci. Health, vol. 39, pp. 353-365, 2004.
[22] C. M. Lino, C. B. F. Azzolini, D. S. V. Nunes, J. M. R. Silva and M. I. N. Silveira, "Methods for the determination of organochlorine pesticide residues in human serum," J. Chromatogr. B, vol. 716, pp. 147-152, 1998.
[23] P. Chulintorn, Ministry of Agriculture and Cooperatives, Bangkok, Thailand, private communication, November 2006.
[24] T. Poolpak, P. Pokethitiyook, M. Kruatrachue, U. Arjarasirikoon and N. Thanwaniwat, "Residue analysis of organochlorine pesticides in the Mae Klong river of Central Thailand," J. Hazard Mater, vol. 156, pp. 230- 239, 2008.
[25] S. Keithmaleesatti, W. Siriwong, P. Varanusupakul, and N. Kitana, "Organochlorine pesticide residues in egg of the snail-eating turtle (Malayemys macrocephala) from the lower Chao Phraya River basin, Thailand," presented at the 7th Int. Symp. Biological Monitoring in Occupational and Environmental Health, Beijing, China, September 10- 12, 2007.
[26] T. H. Wu, J. E. Canas, T. R. Rainwater, S. G. Platt, S. T. McMurry and T. A. Anderson, "Organochlorine contaminants in complete clutches of Morelet-s crocodile (Crocodylus moreletii) eggs from Belize." Environ. Pollut., vol. 144, pp. 151-157, 2006.
[27] S. J. Ormerod and S. J. Tyler, "Environmental pollutants in the eggs of Welsh dippers Cinclus cinclus: a potential monitor of organochlorine and mercury contamination in upland rivers," Bird Study, vol. 37, pp. 171-176, 1990.
[28] R. H. Rauschenberger, M. S. Sep├╝lveda, J. J. Wiebe, N. J. Szabo and T. S. Gross, "Predicting maternal body burdens of organochlorine pesticides from eggs and evidence of maternal transfer in Alligator mississippiensis," Environ. Toxicol. Chem., vol. 23, pp. 2906-2915, 2004.
[29] R. H. Rauschenberger, J. J. Wiebe, J. E. Buckland, J. T. Smith, M. S. Sepulveda and T. S. Gross, "Achieving environmentally relevant organochlorine pesticide concentrations in eggs through maternal exposure in Alligator mississsippiensis." Mar. Environ. Res., vol. 58, pp. 851-856, 2004.
[30] J. E. Canas and T. A. Anderson, "Organochlorine contaminants in eggs: the influence of contaminated nest material," Chemosphere, vol. 47, pp. 585-589, 2002.
[31] S. R. de Solla, C. A. Bishop, G. Van Der Kraak and R. J. Brooks, "Impact of organochlorine contamination on levels of sex hormones and external morphology of common snapping turtles (Chelydra serpentina serpentina) in Ontario, Canada," Environ. Health Perspect., vol. 106, pp. 253-260, 1998.
[32] K. Bang, B. M. Jensen, C. Lydersen and J. C. Skaare, "Organochlorine burdens in blood of ringed and bearded seals from north-western Svalbard," Chemosphere, vol. 44, pp. 193-203, 2001.
[33] J. O. Bustnes, J. C. Skaare, K. E. Erikstad, V. Bakken and F. Mehlum, "Whole blood concentration of organochlorine as a dose metric for studies of the glaucocs gull (Larus hyperboreus)," Environ. Toxicol. Chem., vol. 20, pp. 1046-1052, 2001.
[34] J. M. Keller, J. R. Kucklick, C. A. Harms, and P. D. McClellan-Green, "Organochlorine contaminants in sea turtles: correlations between whole blood and fat," Environ. Toxicol. Chem. vol. 23, pp. 726-38, 2004.
[35] The Association of Official Agricultural Chemists (AOAC) Peer Verified Methods Program, Manual on Policies and Procedures. Arlington, VA: AOAC, 1993.
[36] C. A. Bishop, G. P. Brown, R. J. Brooks, D. R. S. Lean, and J. H. Carey, "Organochlorine contaminant concentrations in eggs and their relationship to body size and clutch characteristics of the female common snapping turtle (Chelydra serpentina serpentina) in Lake Ontario, Canada," Arch. Environ. Contam. Toxicol. vol. 27, pp. 82-87, 1994.