WASET
	%0 Journal Article
	%A Sarun Keithmaleesatti and  Pakorn Varanusupakul and  Wattasit Siriwong and  Kumthorn Thirakhupt and Mark Robson and  Noppadon Kitana
	%D 2009
	%J International Journal of Bioengineering and Life Sciences
	%B World Academy of Science, Engineering and Technology
	%I Open Science Index 28, 2009
	%T Contamination of Organochlorine Pesticides in Nest Soil, Egg, and Blood of the Snail-eating Turtle (Malayemys macrocephala) from the Chao Phraya River Basin, Thailand
	%U https://publications.waset.org/pdf/12138
	%V 28
	%X Organochlorine pesticides (OCPs) are known to be
persistent and bioaccumulative toxicants that may cause reproductive
impairments in wildlife as well as human. The current study uses the
snail-eating turtle Malayemys macrocephala, a long-lived animal
commonly distribute in rice field habitat in central part of Thailand,
as a sentinel to monitor OCP contamination in environment. The
nest soil, complete clutch of eggs, and blood of the turtle were
collected from agricultural areas in the Chao Phraya River Basin,
Thailand during the nesting season of 2007-2008. The novel
methods for tissue extraction by an accelerated solvent extractor
(ASE, for egg) and liquid-liquid extraction (for blood) have been
developed. The nineteen OCP residues were analyzed by gas
chromatography with micro-electron captured detector (GC-μECD).
The validated methods have met requirements of the AOAC
standard. The results indicated that significant amounts of OCPs are
still contaminated in nest soil and eggs of the turtle even though the
OCPs had been banned in this area for many years. This suggested
the potential risk to health of wildlife as well as human in the area.
	%P 193 - 198