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Communities of Ammonia-oxidizing Archaea and Bacteria in Enriched Nitrifying Activated Sludge
Abstract:In this study, communities of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in nitrifying activated sludge (NAS) prepared by enriching sludge from a municipal wastewater treatment plant in three continuous-flow reactors receiving an inorganic medium containing different ammonium concentrations of 2, 10, and 30 mM NH4 +-N (NAS2, NAS10, and NAS30, respectively) were investigated using molecular analysis. Results suggested that almost all AOA clones from NAS2, NAS10, and NAS30 fell into the same AOA cluster and AOA communities in NAS2 and NAS10 were more diverse than those of NAS30. In contrast to AOA, AOB communities obviously shifted from the seed sludge to enriched NASs and in each enriched NAS, communities of AOB varied particularly. The seed sludge contained members of N. communis cluster and N. oligotropha cluster. After it was enriched under various ammonium loads, members of N. communis cluster disappeared from all enriched NASs. AOB with high affinity to ammonia presented in NAS 2, AOB with low affinity to ammonia presented in NAS 30, and both types of AOB survived in NAS 10. These demonstrated that ammonium load significantly influenced AOB communities, but not AOA communities in enriched NASs.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1078569Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1249
 Venter, J.C., Remington, K., Heidelberg, J.F., Halper, A.L., Rusc, D., Eisen, J.A. Environmental genome shotgun sequencing of the Sargasso Sea. Science 304 (2004): 66-74.
 Treusch, A.H., Leininger, S., Kletzin, A., Schuster, S.C., Klenk, H.P., Schleper, C. Novel genes or nitrite reductase and Amo-related proteins indicate a role of uncultivated mesophilic crenarchaeota in nitrogen cycling. Environmental Microbiology 7 (2005): 1985-1995.
 Konneke, M., Bernhard, A.E., de la Torre, J.R., Walker, C.B., Waterbury, J.B., Stahl D.A. Isolation of an autotrophic ammoniaoxidizing marine archaeon. Nature 437 (2005): 543-546.
 Wuchter, C., Abbas, B., Coolen, M.J.L., Herfort, L., van Bleijswijk, J., Timmers, P. Archaeal nitrification in the ocean. Proceeding of the National Academy of Sciences USA 103 (2006): 12317-12322.
 Leininger, S., Urich, T., Schloter, M., Schwark, L., Qi, J., Nicol, G.W. Archaea predominate among ammonia-oxidizing prokaryotes in soils. Nature 442 (2006): 806-809.
 Park, H.D, Wells, G.F., Bae, H., Criddle, C.S., Francis, C.A. Occurrence of ammonia-oxidizing archaea in wastewater treatment plant bioreactors. Applied and Environment Microbiology 72 (2006): 5643-5647.
 Koops, H.P., and Pommerening-Roser A. Distribution and ecophysiology of the nitrifying bacteria emphasizing cultured species. FEMS Microbiology Ecology 37 (2001): 1-9.
 Limpiyakorn, T., Kurisu, F., Sakamoto, Y., and Yagi, O. Effects of ammonium and nitrite on communities and populations of ammoniaoxidizing bacteria in laboratory-scale continuous-flow reactors. FEMS Microbiology Ecology 60 (2007): 501-512.
 Francis, C.A., Roberts, K.J., Beman, J.M., Santoro, A.E., Oakley, B.B. Ubiquity and diversity of ammonia-oxidizing archaea in water columns and sediments of the ocean. Proceeding of the National Academy of Sciences USA 102 (2005): 14683-14688.
 Schloss, P.D., and Handelsman, J. Introducing DOTUR, a computerprogram for defining operational taxonomic units and estimating speciesrichness. Applied and Environment Microbiology 71 (2005): 1501-1506.
 Kowalchuk, G.A. Analysis of ammonia-oxidizing bacteria of the βsubdivision of the class Proteobacteria in costal sand dunes by denaturing gradient gel electrophoresis and sequencing of PCRamplified 16S ribosomal DNA fragments. Applied and Environment Microbiology 63, 4 (1997): 1489-1497.
 Koops, H. P., Purkhold, U., Pommeren, R. A., Timmermann, G., and Wagner, M. The Lithoautotrophic Ammonia-Oxidizing Bacteria. M. Dworkin et al (eds.), The Prokaryotes: An Evolving Electronic Resource For the Microbiological Community, 1-22. New York, 2003.
 Torre, José R., Christopher B. Walker, Anitra E. Ingalls, Martin Könneke, and David A. Stahl. Cultivation of a thermophilic ammonia oxidizingarchaeon synthesizing crenarchaeol. Environmental Microbiology 10,1 (2008): 810-818.
 Santoro, A.E., Francis, C.A., de Sieyes, N.R., and Boehm, A.B. Shifts in the relative abundance of ammonia-oxidizing bacteria and archaea across physicochemical gradients in a subterranean estuary. Environmental Microbioogy 10 (2008): 1068-1079.
 Limpiyakorn T., Shinihara, Y., Kurisu, F., and Yagi, O. Communities of ammonia oxidizing bacteria in activated sludge of various sewage treatment plants in Tokyo. FEMS Microbiology Ecology 54 (2005): 205-117.