Toxicity Study of Two Different Synthesized Silver Nanoparticles on Bacteria Vibrio Fischeri
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Toxicity Study of Two Different Synthesized Silver Nanoparticles on Bacteria Vibrio Fischeri

Authors: E. Binaeian, A.M. Rashidi, H. Attar

Abstract:

A comparative evaluation of acute toxicity of synthesized nano silvers using two different procedures (biological and chemical reduction methods) and silver ions on bacteria Vibrio fischeri was investigated. The bacterial light inhibition test as a toxicological endpoint was used by applying of a homemade luminometer. To compare the toxicity effects as a quantitative parameter, a nominal effective concentrations (EC) of chemicals and a susceptibility constant (Z-value) of bacteria, after 5 min and 30 min exposure times, were calculated. After 5 and 30 min contact times, the EC50 values of two silver nanoparticles and the EC20 values were about similar. It demonstrates that toxicity of silvers was independent of their procedure. The EC values of nanoparticles were larger than those of the silver ions. The susceptibilities(Z- Values) of V.fischeri (L/mg) to the silver ions were greater than those of the nano silvers. According to the EC and Z values, the toxicity of silvers decreased in the following order: Silver ions >> silver nanoparticles from chemical reduction method ~ silver nanoparticles from biological method.

Keywords: Bioluminescence, Luminometer, silver nano particles, Toxicity, Vibrio fischeri

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1077313

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References:


[1] V.K. Sharma, RA Yngard, Y Lin," Silver nanoparticles: Green synthesis and their antimicrobial activities," Adv. Colloid. Interf. Sci., vol. 145,pp 83- 89, 2009.
[2] D . Mandal, M.E.Bolander, D. Mukhopadhyay, G.Sarkar, P. Mukherjee ,"The use of microorganisms for the formation of metal nanoparticles and their application," Appl. Microbiol. Biotechnol.,vol. 69, pp. 485- 491,2006.
[3] Nel, T.Xia ,L. Madler , N. Li," Toxic potential of materials at the nanolevel," Science., vol.311, pp. 622-627,2006.
[4] Barnard , A S," Nanoharzards knowledge is our first defence," Nat. Mater., Vol. 5, pp. 245-248,2006.
[5] S .Sarkar, A.D. Jana, S.K. Samanta, G. Mostafa," Facile synthesis of silver nano particles with highly efficient anti-microbial property,"Polyhedron.,vol. 26,pp.4419- 4428,2007.
[6] A . Ahmad,P.Mukherjee , S.Senapati, D.Mandal, M.I.Khan, R. Kumar, M .Sastry ," Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum Colloids," Surf. B: Biointerf.,vol. 28,pp. 313- 321,2000.
[7] S.W.P .Wijnhoven,W. Peijnenburg, C.A.Herberts, W.I.Hagens, A.G.Oomen, E.H.W.Heugens, B .Roszek, J. Bisschops, I .Gosens, D .Van de Meent, S .Dekkers, W.H.De Jong, M .Van Zijverden, A .Sips, R.E.Geertsma ," Nano-silverÔÇöa review of available data and knowledge gaps in human and environmental risk assessment," Nanotoxicology.,vol. 3,pp. 109-178, 2009.
[8] J .Gao, S.Youn ,A. Hovsepyan, V.L. Llaneza, Y.Wang ,G. Bitton , J.C.J.Bonzongo,"Dispersion and toxicity of selected manufactured nanomaterials in natural river water samples: effects of water chemical composition," Environ Sci.Technol., vol.43,pp. 3322- 3328, 2009.
[9] K.N.J. Stevens, O. Crespo-Biel, E.E.M.van den Bosch, A.A.Dias, M.L.W.Knetsch, Y.B.J.Aldenhoff, F.H.van der Veen, J.G.Maessen, E.E.Stobberingh, L.H.Koole," The relationship between the antimicrobial effect of catheter coatings containing silver nanoparticles and the coagulation of contacting blood," Biomaterials., Vol.30,pp. 3682-3690,2009.
[10] T.M.Benn, P.Westerhoff ," Nanoparticle silver released into water from commercially available sock fabrics," Environ Sci. Technol., Vol.42, pp. 4133-4139,2008.
[11] A .Simon-Deckers, E.Brun, B .Gouget, M.Carriere, C. Sicard-Roselli ," Impact of gold nanoparticles combined to X-ray irradiation on bacteria," Gold Bulletin.,Vol.41,pp. 187-194,2008.
[12] P.V.Asharani, G .Low Kah Mun, M.P.Hande, S. Valiyaveettil ,"Cytotoxicity and genotoxicity of silver nanoparticles in human cells," ACS Nano.,vol. 3,pp. 279-290,2008.
[13] O .Choi, Z.Q.Hu ," Size dependent and reactive oxygen species related nanosilver toxicity to nitrifying bacteria,"Environ Sci. Technol.,vol.42,pp. 4583-4588,2008.
[14] ASTM, Standard Guide for Conducting Daphnia magna Life-Cycle Toxicity Tests. West Conshohocken PA: ASTM International. Standard E 1193 - 97,2004.
[15] T .Li, B .Albee,M. Alemayehu, R.Diaz ,L. Ingham,S. Kamal, M.Rodriguez, S.W.Bishnoi," Comparative toxicity study of Ag, Au, and Ag-Au bimetallic nanoparticles on Daphnia magna," Anal Bioanal Chem., Vol.398,pp. 689-700,2010.
[16] I.Sondi, B.Salopek-Sondi," Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria," J. Colloid Interface Sci. ,vol.275,pp. 177-182,2004.
[17] Lok, C N, Ho CM, R.Chen , Q.Y.He, W.Y.Yu, H.Sun, PK-H .Tam, J.F.Chiu, C.M.Che," Proteomic analysis of the mode of antibacterial action of silver nanoparticles," J. Proteome Res.,vol 5,pp. 916-924,2006.
[18] A .D.Russell, , W.B .Hugo," Antimicrobial activityand action of silver. Prog," Med. Chem.,vol. 31,pp. 351-370,1944.
[19] S. Lee , J.Lee , K.Kim, S.J.Sim, G.M.Bock, J.Yi, J .Lee ," Eco-toxicity of Commercial Silver Nanopowders to Bacterial and Yeast Strains," Biotechnology and Bioprocess Engineering.,vol.14,pp. 490-495,2009.
[20] L .Claudia, U E, Mark, G. Peter, G.R.Edward ," The Vibrio fischeri quorum-sensing systems Ain and Lux sequentially induce luminescence gene expression and are important for persistence in the squid host," Mol. Microbiol.,vol. 50,pp. 319-331,2003.
[21] M.Heinlaan , A. Ivask,I. Blinova, H.C.Dubourguier, A. Kahru ,"Toxicity of nanosized and bulk ZnO, CuO and TiO2 to bacteria Vibrio fischeri and crustaceans Daphnia magna and Thamnocephalus platyurus," Chemosphere. ,vol.71,pp.1308-1316,2008.
[22] M.Mortimer, K.Kasemets, M.Heinlaan, I.Kurvet, A .Kahru,"High throughput kinetic Vibrio fischeri bioluminescence inhibition assay for study of toxic effects of nanoparticles," Toxicology in Vitro.,vol. 22,pp. 1412-1417,2008.
[23] S.Parvez,C. Venkataraman, S.Mukherji,"A review on advantages of implementing luminescence inhibition test (Vibrio fischeri) for acute toxicity prediction of chemicals," Environment International.,vol 32,pp. 265 - 268,2006.
[24] K.Froehner , Backhaus, L.H.Grimme," Bioassay with Vibrio fischeri for the assessment of delayed toxicity," Chemosphere.,vol. 40,pp. 821- 828,2000.
[25] E.Fulladosa, J.C.Murat, Villaescusa," Study on the toxicity of binary equitoxic mixtures of metals using the luminescent bacteria Vibrio fischeri as a biological target," Chemosphere.,vol.58,pp. 551-557,2005.
[26] J.Lappalaineh ,R. Juvonen,J. Nurmi,M. Karp ,"Automated color correction method for Vibrio fischeri toxicity test Comparison of standard and kinetic assays," Chemosphere.,vol. 45,pp.635-641,2001.
[27] D.A.Skoog, F.J.Holler, T.A.Nieman ,"Principles of Instrumental Analysis," vol.5, Fort Worth : Saundres College Press, 1998.
[28] G.Chumanov, K.Sokolov, T.M.Cotton," Unusual extinction spectra of nanometer-sized silver particles arranged in two-dimensional arrays," J Phys. Chem. , vol.100,pp.5166-5168,1996.
[29] Katherine, B H, J.B.Allen,"Interaction of silver (I) ions with the respiratory chain of Escherichia coli: an electrochemical and scanning electrochemical microscopy study of the antimicrobial mechanism of micromolar Ag+," Biochemistry. ,vol.44pp. 13214 -13223,2005.
[30] Yoon, K Y, J.H.Byeon, J.H.Park, J.Hwang," Susceptibility constants of Escherichia coli and Bacillus subtilis to silver and copper nanoparticles," Sci.Total Environ.,vol. 373pp.572-575,2007.