Identification of Phenolic Contents in Malaysian Variety of Pummelo (Citrus Grandis L. Osbeck) Fruit Juice Using HPLC-DAD

Authors: N. N. A. K. Shah, R. A. Rahman, R. Shamsuddin, N. M. Adzahan

Abstract:

Pummelo is known to be the largest of all citrus fruits, with expected ratio of 2:1 (w/v) of producing juice, is an attractive opportunity for Malaysia to expand pummelo-s influence and marketability over the international market of juices. The purpose of this study is to identify and quantify the phenolic compounds in two Malaysian varieties of pummelo fruit juice: Ledang (PO55) and Tambun (PO52). Identifications of polyphenols composition were done using High Performance Liquid Chromatography Diode Array Detection (HPLC-DAD). The phenolic compounds that were found in both varieties were hydroxycinnamic acids and flavonones. This study proved that Tambun variety has the highest antioxidant and phenolic compounds in comparison to Ledang variety. However, considerations have to be made to suit consumer-s taste bud and the amount of enzyme needed to clarify the juice for its marketability.

Keywords: Antioxidant, HPLC, phenolic contents and pummelo fruit juice

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1074745

References:

[1] Amin, I., Norazaidah, Y., Haninida, K.I.E., "Antioxidant activity and phenolic content of raw and blanched Amaranthus species" Food Chem vol 94, pp. 47-52, 2006.
[2] Garg, A., Garg, S., Zeneveld, L.J. & Singla, AK., "Chemistry and pharmacology of the citrus bioflavonoid hesperidin" Photother Res vol 15, pp. 655-669, 2001.
[3] Jayaprakasha, G.K. & Bhimanagouda, S.P., "In vitro evaluation of the antioxidant activities in fruit extracts from citron and blood orange" Food Chem vol 101, pp. 410-418, 2007.
[4] Morton, J.F., "Pummelo" in Fruits of Warm Climates. Miami: Florida Flair Books, 1987, pp. 147-151.
[5] Department of Agriculture Malaysia, "Pomelo: Varieties/clones" unpublished.
[6] Kawaii, S., Tomono, Y., Katase, E., Ogawa, K. & Yano, M., "Quantification of flavonoid constituents in citrus fruits" J Agric. Food vol 47, pp. 3565-3571, 1999.
[7] Bocco, A., Cuvelier, M.E., Richard, H. & Berset, C., "Antioxidant activity and phenolic composition of peel and seed extracts" J Agric. Food vol 41, pp. 2123-2129, 1998.
[8] Kanes, K., Tisserat, B., Berhow, M. & Vandercook, C., "Phenolic composition of various tissues of Rutaceae species" Phytochem vol 32, pp. 961-974, 1993.
[9] Shui, G. & Leong, L.P., " Analysis of polyphenolic ants in star fruit using liquid chromatography and mass spectrometry J Chromato A vol 1022, pp. 67 — 75, 2004.
[10] Strlic, M., Radovic, T., Kolar, J. & Pihlar, B., "Anti and pro-oxidative properties of gallic acid in Fenton-like systems" J Agric. Food vol 50, pp. 6313-6317, 2004.
[11] Nicoli, M.C., Anese, M., Parpinel, M.T., Franceschi, S. & Lerici, C.R., "Study on loss and/or formation of antioxidants during food processing and storage" Cancer Lett. vol 114, pp. 71-74, 1997.