Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 32731
Fluorometric Aptasensor: Evaluation of Stability and Comparison to Standard ELISA Assay

Authors: J. Carlos Kuri, Varun Vij, Raymond J. Turner, Orly Yadid-Pecht

Abstract:

Celiac disease (CD) is an immune system disorder that is related to eating gluten. As gluten-free (GF) diet has become a concern of many people for health reasons, a gold standard had to be nominated. Enzyme-linked immunosorbent assay (ELISA) has taken the seat of this role. However, multiple limitations were discovered, and with that, the desire for an alternative method now exists. Nucleic acid based aptamers have become of great interest due to their selectivity, specificity, simplicity, and rapid-testing advantages. However, fluorescence-based aptasensors have been tagged as unstable, but lifespan details are rarely stated. In this work, the lifespan stability of a fluorescence-based aptasensor is shown over a 8-week long study displaying the accuracy of the sensor and false negatives. This study follows 22 different samples, including GF and gluten-rich (GR) and soy sauce products, off-the-shelf products, and reference material from laboratories; giving a total of 836 tests. The analysis shows an accuracy of correctly classifying GF and GR products of 96.30% and 100%, respectively, when the protocol is augmented with molecular sieves. The overall accuracy remains around 94% within the first 4 weeks and then decays to 63%.

Keywords: Aptasensor, PEG, rGO, FAM, RM, ELISA.

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 309

References:


[1] A. Fasano and C. Catassi, “Celiac disease,” New England Journal of Medicine, vol. 367, no. 25, pp. 2419–2426, 2012.
[2] J. A. King, J. Jeong, F. E. Underwood, J. Quan, N. Panaccione, J. W. Windsor, S. Coward, J. deBruyn, P. E. Ronksley, A. A. Shaheen, H. Quan, J. Godley, S. Veldhuyzen van Zanten, B. Lebwohl, S. C. Ng, J. F. Ludvigsson, and G. G. Kaplan, “Incidence of Celiac Disease Is Increasing Over Time: A Systematic Review and Meta-analysis,” The American journal of gastroenterology, vol. 115, no. 4, pp. 507–525, 4 2020.
[3] B. Niland and B. D. Cash, “Health benefits and adverse effects of a gluten-free diet in non-celiac disease patients,” Gastroenterology and Hepatology, vol. 14, no. 2, pp. 82–91, 2018.
[4] Food and Agriculture Organization of the United Nations and World Health Organization, “Standard for Foods for Special Dietary Use for Persons Intolerant to Gluten,” pp. 1–3, 2008. (Online). Available: http://www.fao.org/fao-who-codexalimentarius/codex-texts/list-standards/ en/
[5] G. Ronald, M. Crystal, Y. Herbert, Y. Horber, M. Reynolds, R. Anthony, and Kalica, “Bronchoalveolar Lavage The Report of an International Conference,” Special report, vol. 90, no. 1, pp. 122–131, 1983.
[Online]. Available: http://dx.doi.org/10.1378/chest.90.1.122
[6] G. E. Rodriguez and M. C. Dyson, “Diagnosis of Allergic Disease,” Primary Care: Clinics in Office Practice, vol. 14, no. 3, pp. 447–455, 9 1987. (Online). Available: https://linkinghub.elsevier.com/retrieve/pii/S0095454321010162
[7] D. Barisani, Y. Junker, S. Vanessi, R. Meneveri, M. T. Bardella, D. Schuppan, R. Plaza-Silva, M. Laura, L. Lordello, I. Nishitokukado, C. Lucia Ortiz-Agostinho, F. M. Santos, A. Z. Leite, and A. M. Sipahi, “S2035 Detection and Quantification of Gluten in Processed Food by ELISA in Brazil,” Aga Abstracts, 2010.
[8] L. A´ lvaro Macedo, “Ana´lise da Presenc¸a de Glu´ten em Alimentos Rotulados como Livres de Gl´uten Atrav´es de Ensaio Imunoenzim´atico e de Fitas Imunocromatogr´aficas,” Ph.D. dissertation, Universidade Federal do Rio Grande do Sul, 2010.
[9] T. Thompson and S. Simpson, “A comparison of gluten levels in labeled gluten-free and certified gluten-free foods sold in the United States,” pp. 143–146, 2015.
[10] L. Stevens and M. Rashid, “Gluten-free and regular foods: A cost comparison,” Canadian Journal of Dietetic Practice and Research, vol. 69, no. 3, pp. 147–150, 2008.
[11] S. N. Diaz, V. Vij, R. Turner, and O. Yadid-Pecht, “Simplified Gluten Detection Approach in the Presence of a Black Hole Quencher (BHQ),” SPIE, vol. 11900, no. 119003E, 2021.
[12] K. A. Scherf and R. E. Poms, “Recent developments in analytical methods for tracing gluten,” Journal of Cereal Science, vol. 67, pp. 112–122, 2016.
[13] S. M. Shaban and D. H. Kim, “Recent advances in aptamer sensors,” Sensors (Switzerland), vol. 21, no. 3, pp. 1–31, 2021.
[14] P. R¨othlisberger and M. Hollenstein, “Aptamer chemistry,” Advanced Drug Delivery Reviews, vol. 134, pp. 3–21, 2018. (Online). Available: https://doi.org/10.1016/j.addr.2018.04.007
[15] K. Y. Goud, K. K. Reddy, M. Satyanarayana, S. Kummari, and K. V. Gobi, “A review on recent developments in optical and electrochemical aptamer-based assays for mycotoxins using advanced nanomaterials,” Microchimica Acta, vol. 187, no. 1, 2020.
[16] P. Zuo, X. Li, D. C. Dominguez, and B. C. Ye, “A PDMS/paper/glass hybrid microfluidic biochip integrated with aptamer-functionalized graphene oxide nano-biosensors for one-step multiplexed pathogen detection,” Lab on a Chip, vol. 13, no. 19, pp. 3921–3928, 10 2013.
[17] M. Xhaferaj, T. O. Alves, M. S. L. Ferreira, K. A. Scherf, O. Thais, Alves, M. S. L. Ferreira, and K. A. Scherf, “Recent progress in analytical method development to ensure the safety of gluten-free foods for celiac disease patients,” Journal of Cereal Science, vol. 96, p. 103114, 2020. (Online). Available: https://doi.org/10.1016/j.jcbs.2020.08.008 https://doi.org/10.1016/j.jcs.2020.103114
[18] “Integrated DNA Technologies.” (Online). Available: https://www.idtdna.com
[19] “Food & Feed Analysis - a division of R-Biopharm AG.” (Online). Available: https://food.r-biopharm.com/
[20] “Trilogy Analytical Laboratory.” (Online). Available: https://trilogylab.com/
[21] “MilliporeSigma.” (Online). Available: https://www.sigmaaldrich.com
[22] Y. Seok Kim, N. H. Ahmad Raston, and M. Bock Gu, “Aptamer-based nanobiosensors,” Biosensors and Bioelectronics, vol. 76, pp. 2–19, 2016. (Online). Available: http://dx.doi.org/10.1016/j.bios.2015.06.040
[23] S. Amaya-Gonza´lez, N. De-Los-Santos-A´ lvarez, A. J. Miranda-Ordieres, and M. J. Lobo-Casta˜n´on, “Aptamer binding to celiac disease-triggering hydrophobic proteins: A sensitive gluten detection approach,” Analytical Chemistry, vol. 86, no. 5, pp. 2733–2739, 2014.
[24] N. Suresh, “Fluorescence-based gluten detection using aptamer hybrid,” Ph.D. dissertation, University of Calgary, 2019. (Online). Available: http://hdl.handle.net/1880/110145
[25] Z. S. Pehlivan, M. Torabfam, H. Kurt, C. Ow-Yang, N. Hildebrandt, and M. Y¨uce, “Aptamer and nanomaterial based FRET biosensors: a review on recent advances (2014–2019),” Microchimica Acta, vol. 186, no. 8, 2019.
[26] L. Cui, J. Wu, and H. Ju, “Label-free signal-on aptasensor for sensitive electrochemical detection of arsenite,” Biosensors and Bioelectronics, vol. 79, pp. 861–865, 2016. (Online). Available: http://dx.doi.org/10.1016/j.bios.2016.01.010
[27] S. Amaya-Gonza´lez, N. de-los Santos-A´ lvarez, A. J. Miranda-Ordieres, and M. J. Lobo-Casta˜n´on, “Sensitive gluten determination in gluten-free foods by an electrochemical Aptamer-based assay,” Analytical and Bioanalytical Chemistry, vol. 407, no. 20, pp. 6021–6029, 2015.
[28] R-Biopharm AG, “Enzyme immunoassay for the quantitative analysis of gliadins and corresponding prolamines,” AOAC Research Institute, Darmstadt, Germany, Tech. Rep., 2016.
[29] Federal Register, “Rules and Regulations: 49240,” The State University of New Jersey, New Jersey, Tech. Rep. 157, 2020.