J. Carlos Kuri and Varun Vij and Raymond J. Turner and Orly Yadid-Pecht
Fluorometric Aptasensor Evaluation of Stability and Comparison to Standard ELISA Assay
113 - 117
2022
16
11
International Journal of Nutrition and Food Engineering
https://publications.waset.org/pdf/10012811
https://publications.waset.org/vol/191
World Academy of Science, Engineering and Technology
Celiac disease (CD) is an immune system disorder that is related to eating gluten. As glutenfree (GF) diet has become a concern of many people for health reasons, a gold standard had to be nominated. Enzymelinked immunosorbent assay (ELISA) has taken the seat of this role. However, multiple limitations were discovered, and with that, the desire for an alternative method now exists. Nucleic acid based aptamers have become of great interest due to their selectivity, specificity, simplicity, and rapidtesting advantages. However, fluorescencebased aptasensors have been tagged as unstable, but lifespan details are rarely stated. In this work, the lifespan stability of a fluorescencebased aptasensor is shown over a 8week long study displaying the accuracy of the sensor and false negatives. This study follows 22 different samples, including GF and glutenrich (GR) and soy sauce products, offtheshelf products, and reference material from laboratories; giving a total of 836 tests. The analysis shows an accuracy of correctly classifying GF and GR products of 96.30 and 100, respectively, when the protocol is augmented with molecular sieves. The overall accuracy remains around 94 within the first 4 weeks and then decays to 63.
Open Science Index 191, 2022