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Influence of Organic Supplements on Shoot Multiplication Efficiency of Phaius tankervilleae var. alba

Authors: T. Punjansing, M. Nakkuntod, S. Homchan, P. Inthima, A. Kongbangkerd


The influence of organic supplements on growth and multiplication efficiency of Phaius tankervilleae var. alba seedlings was investigated. 12 week-old seedlings were cultured on half-strength semi-solid Murashige and Skoog (MS) medium supplemented with 30 g/L sucrose, 8 g/L agar and various concentrations of coconut water (0, 50, 100, 150 and 200 mL/L) combined with potato extract (0, 25 and 50 g/L) and the pH was adjusted to 5.8 prior to autoclaving. The cultures were then kept under constant photoperiod (16 h light: 8 h dark) at 25 ± 2 °C for 12 weeks. The highest number of shoots (3.0 shoots/explant) was obtained when cultured on the medium added with 50 ml/L coconut water and 50 g/L potato extract whereas the highest number of leaves (5.9 leaves/explant) and roots (6.1 roots/explant) could receive on the medium supplemented with 150 ml/L coconut water and 50 g/L potato extract. with 150 ml/L coconut water and 50 g/L potato extract. Additionally, plantlets of P. tankervilleae var. alba were transferred to grow into seven different substrates i.e. soil, sand, coconut husk chip, soil-sand mix (1: 1), soil-coconut husk chip mix (1: 1), sand-coconut husk chip mix (1: 1) and soil-sand-coconut husk chip mix (1: 1: 1) for four weeks. The results found that acclimatized plants showed 100% of survivals when sand, coconut husk chip and sand-coconut husk chip mix are used as substrates. The number of leaves induced by sand-coconut husk chip mix was significantly higher than that planted in other substrates (P > 0.05). Meanwhile, no significant difference in new shoot formation among these substrates was observed (P < 0.05). This precursory developing protocol was likely to be applied for more large scale of plant production as well as conservation of germplasm of this orchid species.

Keywords: Acclimatization, coconut water, orchid, Phaius tankervilleae var. alba., potato extract.

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[1] R. L. Dressler, Phylogeny and classification of orchid family. Portland, OR: Dioscorides, 1993, ch1.
[2] H. Kurzweil, “A precursory study of the Calanthe group Orchidaceae in Thailand,” Adasonia ser, vol. 3, no. 1, pp. 92, 2010.
[3] L.V. Averyanov and E.A. Christenson, “Orchids of Vietnam. Phaius tankervilleae,” Orchids. Mag. Amer. Orch. Soc., vol. 67, no 4, pp. 368-370, 1998.
[4] R Ngapui, P. Gogoi, K. Chowlu, and S. P. Vij, “Effects of NPK and 6-Benzylaminopurine on Growth and Flowering of Two Orchid Genera,” Pl sci Res, vol. 3, no 1, pp. 93-99, 2018.
[5] M. M. Hossain, “Traditional therapeutic uses of some orchids of Bangladesh,” Medicinal Aromatic Plant Sci. Biotech., vol.3, no1, pp.100-106, 2009.
[6] T. Hirano, T. Godo, K. Miyoshi, K. Ishikawa M. Ishikawa and M. Mii, “Cryopreservation and low temperature storage of seeds of Phaius tankervilleae,” Plant Biotechnol Rep., vol.3, pp. 103-109, 2009.
[7] R. Thokchom, S. Maitra and S. Sharma, “In vitro Mass Propagation of Endangered Terrestrial Orchid - Phaius tankervilleae (L’Her.) Blume through Green Seed Pod Culture,” Microbiol. App. Sci., vol. 6, no.5, pp.722-728, 2017.
[8] D. J. Metcalfe and T.J. Lawson, “An International Union for Conservation of Nature risk assessment of coastal lowland rainforests of the Wet Tropics Bioregion, Queensland,” Austral Ecology., vol. 40, no. 4, pp.373-385, 2015.
[9] UNEP-WCMC (Comps.), Checklist of CITES species. CITES Secretariat, Geneva, Switzerland, and UNEP-WCMC, Cambridge, United Kingdom. Accessed on September 15, 2018. 2014.
[10] S. Chugh, S. Guha, and I. U. Rao, “Micropropagation of orchids: A review on the potential of different explants,” Sci. Hortic., vol. 122, no. 4, pp. 507-520, 2009.
[11] W. Pakum, S. Watthana, K. Srimuang, and A. Kongbangkerd, “Influence of Medium Component on İn vitro Propagation of Thais Endangered Orchid: Bulbophyllum nipondhii Seidenf,” Plant Tissue Cult. & Biotech., vol. 26, no.1, pp.37- 46, 2016.
[12] B. Chen, S. J. Trueman, L. Jianmin, L. Qianzhen, H. Fan and J. Zhang, “Micropropagation of the endangered medicinal orchid, Dendrobium officinale,” Life Sci., vol.11, no.9, pp.526-530, 2014.
[13] Z. Molnár, E. Virág and V. Ördög, “Natural substances in tissue culture media of higher plants,”. Acta Biol Szeged., vol.55, no.1, pp. 123-127, 2011.
[14] B. Pant, S. Shrestha and S. W. Pradhan, “In Vitro seed Germination and Seeding Development of Phaius tancarvillere (L’ Her.) Blume. Scientific World, vol. 9, pp. 50 – 52, 2011.
[15] B. Pant and S. Shrestha “In Vitro Mass Propagation of a Ground Orchid Phaius tancarvillere (L’ Her.) Blume through Shoot Tip Culture. Plant Tissue Cult. & Biotech, vol. 21, pp. 181- 188, 2011.
[16] F. Vacin, and F. W. Went, “some pH changes in nutrient solution.” Botanic Gardens Conservation News., vol. 110, pp. 605-613, 1949.
[17] T. Murashige and F. Skoog, “A revised medium for rapid growth and bioassay with tobacco tissue cultures,” Physiol Plant, vol.15, no. 3, pp. 473 - 497, 1962.
[18] Z. Molnár, E Virág, and V Ördög “Natural substances in tissue culture media of higher plants”. Acta Biol Szeged, vol.55, no1, pp.123-127, 2011.
[19] T.A. Thorpe, C. Stasolla, E.C. Yeung, G.J. de Klerk, A. Roberts and E.F. George, “The components of plant tissue culture media II: Organic addition, osmotic and pH effects, and support systems, Springer-Verlag, 3rd ed, vol.1, pp. 115-173, 2008.
[20] M. A. Baque, Y.K. Shin, T. Elshmari, E.J. Lee and K.Y. Paek, “Effect of light quality, sucrose and coconut water concentration on the microporpagation of Calanthe hybrids ‘Bukduseong’ × ‘Hyesung’ and ‘Chunkwang’ × ‘Hyesung’)”, Aust J Crop Sci vol. 5, no10, pp.1247-1254, 2011.
[21] D. Puchooa, “Comparison of different culture media for the in vitro culture of Dendrobium (orchidaceae)”. Int J Agri Biol, vol. 6, no.5, pp.884-888, 2004.
[22] S. Dohling, S. Kumaria and P. Tandon, “Multiple shoot induction from axillary bud culture of the medicinal orchid, Dendrobium longicornu”., AoB Plants, pp.1-7, 2012.
[23] JWH Yong, L. Ge, Y. F. Ng and S. N. Tan, “The chemical composition and biological properties of coconut (Cocos nucifera L.) Water”. Molecules vol.14, no.5, pp.144-5164, 2009
[24] V. Bartova and J. Barta, “Chemical composition and nutritional value of protein concentrates isolated from potato (Solanum tuberosum L.) fruit juice by precipitation with ethanol or ferric chloride”. J Agric Food Chem, vol.57, pp.9028-9034, 2009.
[25] S. Malmgren, “Large-scale asymbiotic propagation of Cypripedium calceolus plant physiology from a surgeons point of view”. Botanical Garden Micropropagation News, vol.1, pp.59-63, 1992.
[26] B. N. Hazarika, “Acclimatization of tissue-cultured plants,”. Curr Sci vol, 85, no.12, pp.1704-1712, 2003.
[27] L. M. Moraes, R. T. Faria and F. L Cuquel, “Activated charcoal for in vitro propagation of Brazilian orchids,” Acta Hortic, vol. 683, pp. 383-390, 2005.
[28] S Dohling, S Kumaria and P Tandon, “Multiple shoot induction from axillary bud culture of the medicinal orchid, Dendrobium longicornu,”. AoB Plants ,vol. 17, 2012.