Phytochemical Analysis and Antioxidant Activity of Colocasia esculenta (L.) Leaves
Colocasia esculenta leaves and roots are widely used in Asian countries, such as, India, Srilanka and Pakistan, as food and feed material. The root is high in carbohydrates and rich in zinc. The leaves and stalks are often traditionally preserved to be eaten in dry season. Leaf juice is stimulant, expectorant, astringent, appetizer, and otalgia. Looking at the medicinal uses of the plant leaves; phytochemicals were extracted from the plant leaves and were characterized using Fourier-transform infrared spectroscopy (FTIR) to find the functional groups. Phytochemical analysis of Colocasia esculenta (L.) leaf was studied using three solvents (methanol, chloroform, and ethanol) with soxhlet apparatus. Powder of the leaves was employed to obtain the extracts, which was qualitatively and quantitatively analyzed for phytochemical content using standard methods. Phytochemical constituents were abundant in the leave extract. Leaf was found to have various phytochemicals such as alkaloids, glycosides, flavonoids, terpenoids, saponins, oxalates and phenols etc., which could have lot of medicinal benefits such as reducing headache, treatment of congestive heart failure, prevent oxidative cell damage etc. These phytochemicals were identified using UV spectrophotometer and results were presented. In order to find the antioxidant activity of the extract, DPPH (2,2-diphenyl-1-picrylhydrazyl) method was employed using ascorbic acid as standard. DPPH scavenging activity of ascorbic acid was found to be 84%, whereas for ethanol it was observed to be 78.92%, for methanol: 76.46% and for chloroform: 72.46%. Looking at the high antioxidant activity, Colocasia esculenta may be recommended for medicinal applications. The characterizations of functional groups were analyzed using FTIR spectroscopy.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.3461842Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 667
 S. K. Mengane, “Antifungal activity of the crude extracts of Colocasia esculenta leaves in vitro on plant pathogenic fungi,” International Research Journal of Pharmacy, 2015, Vol. 6, Issue 10, pp. 713-714.
 Nakade Dhanraj B, Mahesh S. Kadam, Kiran N. Patil and Vinayak S. Mane, “Phytochemical screening and Antibacterial Activity of Western Region wild leaf Colocasia esculenta,” International Research Journal of Biological Sciences, 2013, Vol. 2(10), pp. 18-21.
 Odedeji J. O., Oyeleke G. O., Ayinde L. A. and Azeez L. A., “Nutritional, Antinutritional Compositions and Organoleptic Analyses of Raw and Blanched Cocoyam (Colocasia esculenta) Leaves,” IOSR Journal of Environmental Science, Toxicology and Food Technology, 2014, Vol. 8, Issue 2, pp 45-48.
 Azubike Nkiruka Chinonyelum, Okwuosa Chukwugozie Nwachukwu, Nwachukwu Daniel Chukwu, Onyemelukwe Anulika Obianuju, Onwukwe Okechukwu Steven, Chukwu Ikechukwu John Paul, Orji Oliver, Ojiakor Nkiru Peace and Achukwu Peter Uwadiegwu, “in vivo Hepatoprotective studies on saponin and alkaloid rich fractions isolated from Colocasia esculenta (L. schott) leaves,” Pharmacology Online, 2017, Vol. 2, pp 66-74.
 Pritha Chakraborty, Papiya Deb, Sudeshna Chakraborty, Bohnisikha Chatterjee and Jayanthi Abraham, “Cytotoxicity and antimicrobial activity of Colocasia esculenta,” Journal of Chemical and Pharmaceutical Research, 2017, Vol. 7(12), pp. 627-635.
 O. Victor Njoku and Chidi Obi, “Phytochemical constituents of some selected medicinal plants,” African Journal of Pure & Applied Chemistry, 2009, Vol. 3(11) pp. 228-233.
 Shoib A. Baba & Shahid A. Malik, “Determination of phenolic and flavonoid content, antimicrobial and antioxidant activity of a root extract of Arisaema jacquemontii,” 2015, Journal of Taibah University of Science, vol. 9, pp. 449-454.
 Saritha Surapaneni and Prakash T, “Comprehensive Assignments of Extraction, Isolation and Characterization of Taraxerol from Bark Annona reticulata L. and Chemopreventive Effect on Human Prostate Cancer Cell Lines,” Carcinogenesis and Mutagenesis, 2018, Vol. 9, Issue 1, pp. 1-8.
 Padmasri Govindarajan and Sarada D.V.L, “Isolation and characterization of stigmasterol and b-sitosterol from Acacia nilotica (l.) Delile ssp Indica (benth.) Brenan,” Journal of Pharmacy Research, 2011, Vol. 4, Issue 10, pp. 3601-3602.
 Bhagyashree R Patil and Hussein M Ageely, “Antihepatotoxic Activity of Colocasia Esculenta Leaf Juice,” International Journal of Advanced Biotechnology and Research, 2011, Vol. 2, Issue 2, pp. 296-304.