Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 32451
A Simplified, Fabrication-Friendly Acoustophoretic Model for Size Sensitive Particle Sorting

Authors: V. Karamzadeh, J. Adhvaryu, A. Chandrasekaran, M. Packirisamy


In Bulk Acoustic Wave (BAW) microfluidics, the throughput of particle sorting is dependent on the complex interplay between the geometric configuration of the channel, the size of the particles, and the properties of the fluid medium, which therefore calls for a detailed modeling and understanding of the fluid-particle interaction dynamics under an acoustic field, prior to designing the system. In this work, we propose a simplified Bulk acoustophoretic system that can be used for size dependent particle sorting. A Finite Element Method (FEM) based analytical model has been developed to study the dependence of particle sizes on channel parameters, and the sorting efficiency in a given fluid medium. Based on the results, the microfluidic system has been designed to take into account all the variables involved with the underlying physics, and has been fabricated using an additive manufacturing technique employing a commercial 3D printer, to generate a simple, cost-effective system that can be used for size sensitive particle sorting.

Keywords: 3D printing, 3D microfluidic chip, acoustophoresis, cell separation, MEMS, microfluidics.

Digital Object Identifier (DOI):

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 762


[1] I. Leibacher, S. Schatzer, and J. Dual, “Impedance matched channel walls in acoustofluidic systems,” Lab Chip, vol. 14, no. 3, pp. 463–470, 2014.
[2] C. W. Shields IV, C. D. Reyes, and G. P. López, “Microfluidic cell sorting: a review of the advances in the separation of cells from debulking to rare cell isolation,” Lab Chip, vol. 15, no. 5, pp. 1230–1249, 2015.
[3] G. Comina, A. Suska, and D. Filippini, “PDMS lab-on-a-chip fabrication using 3D printed templates,” Lab Chip, vol. 14, no. 2, pp. 424–430, 2014.
[4] R. Amin, S. Knowlton, A. Hart, B. Yenilmez, and F. Ghaderinezhad, “3D-printed microfluidic devices,” Biofabrication, vol. 8, no. 2, pp. 1–16.
[5] A. Ahmadian, Y. Adam, P. William, and W. Tammy, “3D printing : an emerging tool for novel microfluidics and lab‑on‑a‑chip applications,” Microfluid. Nanofluidics, 2016.
[6] S. Waheed et al., “3D printed microfluidic devices: enablers and barriers,” Lab Chip, vol. 16, no. 11, pp. 1993–2013, 2016.
[7] I. Leibacher, P. Reichert, and J. Dual, “Microfluidic droplet handling by bulk acoustic wave (BAW) acoustophoresis,” Lab Chip, vol. 15, no. 13, pp. 2896–2905, 2015.
[8] P. B. Muller, R. Barnkob, M. J. H. Jensen, and H. Bruus, “A numerical study of microparticle acoustophoresis driven by acoustic radiation forces and streaming-induced drag forces,” Lab Chip, vol. 12, no. 22, p. 4617, 2012.
[9] M. Antfolk, P. B. Muller, P. Augustsson, H. Bruus, and T. Laurell, “Focusing of sub-micrometer particles and bacteria enabled by two-dimensional acoustophoresis,” Lab Chip, vol. 14, no. 15, pp. 2791–2799, 2014.
[10] P. Augustsson, J. T. Karlsen, and H. Bruus, “Acoustophoretic manipulation of sub-micron objects enabled by density gradients,” in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, 2016, pp. 158–159.
[11] P. Augustsson, J. T. Karlsen, H.-W. Su, H. Bruus, and J. Voldman, “Iso-acoustic focusing of cells for size-insensitive acousto-mechanical phenotyping.,” Nat. Commun., vol. 7, no. May, p. 11556, 2016.
[12] H. N. Chan, Y. Chen, Y. Shu, Y. Chen, Q. Tian, and H. Wu, “Direct, one-step molding of 3D-printed structures for convenient fabrication of truly 3D PDMS microfluidic chips,” Microfluid. Nanofluidics, vol. 19, no. 1, pp. 9–18, 2015.