Growth Performance and Yield of the Edible White Rot Fungus (Pleurotus ostreatus) on Different Agro Waste Materials
A study was carried out to evaluate the growth and yield performance of Pleurotus ostreatus spawn on different organic substrates in Lafia, Nasarawa State, Nigeria. 50 g each of four different substrates namely; corncobs, rice straw, sugarcane bagasse and sawdust sourced locally from farmlands and processing sites, were amended with 2% calcium carbonate and calcium sulphide and sterilized using three sterilization methods namely; hot water, steam, and lime. Five grams of P. ostreatus spawn were inoculated unto treated substrates, incubated in the dark for 16 days and in light for 19 days at 25 0C for the commencement of pinhead and fruit body formations respectively. Growth and yield parameters such as days to full colonization, days to pinhead formation and days to fruit body formation were recorded. Cap diameter and fresh weight of mature mushrooms were also measured for a total count of four flushes. P. ostreatus spawn grown on sugarcane bagasse recorded the highest mean cap diameter (4.69 cm), highest mean fresh weight (34.68 g), highest biological efficiency (69.37%) and highest production rate (2.83 g per day). Spawn grown on rice straw recorded the least number of days to full substrate colonization (11.00). Spawn grown on corn cobs recorded the least mean number of days to pin head (18.75) and fruiting body formations (20.25). There were no significant differences (P ≤ 0.05) among the evaluated substrates with respect to growth and yield performance of P. ostreatus. Substrates sterilized with hot water supported the highest mean cap diameter (5.64 cm), highest biological efficiency (87.04%) and highest production rate (3.43 g per day) of P. ostreatus. Significant differences (P ≤ 0.05) were observed in cap diameter, fresh weight, biological efficiency and production rates among the evaluated sterilization methods. Hot water sterilization of sugarcane bagasse could be adopted for enhanced yield of oyster mushrooms, especially among indigent farming communities in Nigeria and beyond.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1314899Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 688
 S.T. Chang, and J.A. Buswell, “Ganoderma lucidum (Curt. Fr.) P. Karst. (Aphyllophoro mycetidease): A mushrooming medicinal mushroom”, International Journal of Medicinal Mushrooms, vol. 1, 1999, pp. 139–146.
 S.K. Ogunlana, “Studies on the cultivation of the edible mushroom Plerotus tuber regium (Fr) singer”, Tropical Journal of Applied Sciences, vol. 2, 1992, pp. 56-60.
 P.A. Stamets, “Novel approach to farm waste management”, Mushroom Journal Winter, vol. 30, 2001, pp. 28-32.
 S.S. Patil, R.M. Kadam, S.L. Shinde, and S.A. Deshmukh, “Effect of different substrates on productivity and proximate composition of Pleurotus florida”, International Journal of Plant Sciences, vol. 3, no. 1, 2008, pp. 151-153.
 O. Peter, Nutritional aspects and medicinal use Manual on Mushroom Cultivation, Amsterdam: Tool Foundation, 1991, pp. 23-24.
 E.N. Ekpo, and A.P. Aluko, Cultivation strategies and nutritive values of edible Mushrooms (Lentinus tuber-regium) as a component of sustainable livelihood, In Proceedings of 28th Annual conference of Forestry Association of Nigeria, Akure Ondo State, J.E. Abu, and L. Popoola, Eds. 2002, pp. 114-118.
 A. Rehana, T. Muhammad, and R. Tahir, “Propagation of Pleurotus sajor-caju (Oyster mushroom) through tissue”, Pakistan Journal of Botany, vol. 39, no. 4, 2007, pp. 1383-1386.
 B.A. Oso, “Pleurotus tuber- regium from Nigeria”, Mycologia, vol. 69, 1977, pp. 271-279.
 C.I. Onuoha, “Cultivation of mushroom (Pleurotus tuber regium) using some local substrates”, Life Science Journal, vol. 4, no. 4, 2007, pp. 58-61.
 I.O. Fasidi, and V. Ekuere, “Studies on Pleurotus tuberregium (Fr) Sing; cultivation, proximate composition of stored mushrooms”, Food Chem., vol. 55, 1993, pp. 165-168.
 T. Hussain, “Growing mushroom a new horizon in agriculture”, Mushroom Journal, vol. 21, 2001, pp. 23-6.
 S.T. Chang, O.W. Lau, and K.Y. Cho, “The cultivation and nutritional value of Pleurotus sajor-caju”, European Journal of Applied Microbiology and Biotechnology, vol. 12, 1981, pp. 58–62.
 J.W. Kimenju, G.O.M. Odero, E.W. Mutitu, P.M. Wachira, R.D. Narla, and W.M. Muiru, “Suitability of locally available substrates for oyster mushroom (Pleurotus ostreatus) cultivation in Kenya”, Asian Journal of Plant Sciences, vol. 8, no. 7, 2009, pp. 510-514.
 I.O. Fasidi, and K.S. Olorunmaje, “Studies on the requirements for vegetative growth of pleurotus tuber regium (Fr) singer”, Nigerian Mushroom food chemistry, vol. 50, 1994, pp. 397-401.
 S.M. Ayodele, E.O. Akpaja, and F. Anyiador, “Evaluation of the yield of Lentinus squarrosulus (Mont) Singer on selected economic tree species”, Pakistan Journal of Biological Sciences, vol. 10, no. 23, 2007, pp. 4283-4286.
 A.N. Philippoussis, P.A. Diamantopoulou, and G.I. Zervakis, “Correlation of the properties of several lignocellulosic substrates to the crop performance of the shiitake mushroom Lentinula edodes”, World Journal of Microbiology and Biotechnology, vol. 19, no. 6, 2003, pp. 551-557.
 A. Tripathy, T.K. Sahoo, and S.R. Behera, “Yield evaluation of paddy straw mushrooms (Volvariella spp.) on various lignocellulosic wastes”, Botany Research International, vol. 4, no. 2, 2011, pp. 19-24.
 R. Tinoco, M.A. Pickard, and R. Vazquez-Duhalt, “Kinetic differences of purified laccases from six Pleurotus ostreatus strains”, Letters in Applied Microbiology, vol. 32, no. 5, 2001, pp. 331-335.
 B.O. Onyango, V.A. Palapala, P.F. Arama, S.O. Wagai, and B.M. Gichimu, “Suitability of selected supplemented substrates for cultivation of Kenyan native wood ear mushrooms (Auricularia auricula)”, American Journal of Food Technology, vol. 6, no. 5, 2011, pp. 395-403.
 G. Zervakis, A. Philippoussis, S. Ioannidou, and P. Diamantopoulou, “Mycelium growth kinetics and optimal temperature conditions for the cultivation of edible mushroom species on lignocellulosic substrates”, Folia microbiological, vol. 46, no. 3, 2001, pp. 231-234.
 D. Taurachand, Oyster mushroom cultivation, Republic of Korea: Mush World, 2004, pp. 118-121.
 G.V. Thomas, S.R. Prabhu, M.Z. Reeny, and B.M. Bopaiah, “Evaluation of lignocellulosic biomass from coconut palm as substrate for cultivation of Pleurotus sajor-caju (Fr.) Singer”, World Journal of Microbiology and Biotechnology, vol. 14, no. 6, 1998, pp. 879-882.
 B.K. Pani, and S.R. Das, “Effect of pre-treatment of substrate on the yield of oyster mushroom Pleurotus sajor-caju (Fr. Singer)”, Journal of Mycopathological Research, vol. 36, no. 2, 1998, pp. 113-114.
 D. Ejigu, and T. Kebede, “Effect of Different Organic Substrates and their Pasteurization Methods on Growth Performance, Yield and Nutritional values of Oyster Mushroom (Pleurotus ostreatus)”, Global Journal of Science Frontier Research, vol. 15, no. 6, 2015, pp. 27-29.
 J.S. Mejia, and E. Alberto, “Heat treatment of wheat straw by immersion in hot water decreases mushroom yield in Pleurotus ostreatus”, Revista Iberoamericana de Micología, vol. 30, no. 2, 2013, pp. 125-129.