Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 32759
Microfluidic Paper-Based Electrochemical Biosensor

Authors: Ahmad Manbohi, Seyyed Hamid Ahmadi

Abstract:

A low-cost paper-based microfluidic device (PAD) for the multiplex electrochemical determination of glucose, uric acid, and dopamine in biological fluids was developed. Using wax printing, PAD containing a central zone, six channels, and six detection zones was fabricated, and the electrodes were printed on detection zones using pre-made electrodes template. For each analyte, two detection zones were used. The carbon working electrode was coated with chitosan-BSA (and enzymes for glucose and uric acid). To detect glucose and uric acid, enzymatic reactions were employed. These reactions involve enzyme-catalyzed redox reactions of the analytes and produce free electrons for electrochemical measurement. Calibration curves were linear (R² > 0.980) in the range of 0-80 mM for glucose, 0.09–0.9 mM for dopamine, and 0–50 mM for uric acid, respectively. Blood samples were successfully analyzed by the proposed method.

Keywords: Multiplex, microfluidic paper-based electrochemical biosensors, biomarkers, biological fluids.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1132645

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1516

References:


[1] S. K. Sia, L. J. Kricka, Microfluidics and point-of-care testing, Lab Chip, vol. 8, pp. 1982–1983, 2008.
[2] C. D. Chin, V. Linder, S. K. Sia, Lab-on-a-chip devices for global health: Past studies and future opportunities, Lab Chip, vol. 7, pp. 41–57, 2007.
[3] P. Yager, T. Edwards, E. Fu, K. Helton, K. Nelson, M. R. Tam, B. H. Weigl, Microfluidic diagnostic technologies for global public health, Nature, vol. 442, pp. 412–418, 2006.
[4] W. Dungchaia, O. Chailapakul, C. S. Henry, Use of multiple colorimetric indicators for paper-based microfluidic devices, Anal. Chim. Acta, vol. 674 pp. 227–233, 2010.
[5] X. Mao, M. Baloda, A. S. Gurung, Y. Lin, G. Liu, Multiplex electrochemical immunoassay using gold nanoparticle probes and immunochromatographic strips, Electrochem. Commun., vol. 10, pp. 1636–640, 2008.
[6] A. W. Martinez, S. T. Phillips, E. Carrilho, S. W. Thomas, H. Sindi, G. M. Whitesides, Simple Telemedicine for Developing Regions: Camera Phones and Paper-Based Microfluidic Devices for Real-Time, Off-Site Diagnosis, Anal. Chem., vol. 80, pp. 3699–3707, 2008.
[7] A. W. Martinez, S. T. Phillips, M. J. Butte, G. M. Whitesides, Patterned Paper as a Platform for Inexpensive, Low-Volume, Portable Bioassays, Angew. Chem. Int. Ed., vol. 46, pp. 1318–1320, 2007.
[8] W. Dungchai, O. Chailapakul, C. S. Henry, Electrochemical detection for paper-based microfluidics, Anal Chem., vol. 81, pp. 5821–5826, 2009.
[9] X. Li, J. Tian, T. Nguyen, W. Shen, Paper-Based Microfluidic Devices by Plasma Treatment, Anal. Chem., vol. 80, pp. 9131–9134, 2008.
[10] One Step Drugs of Abuse Test, Core Technology Co., Ltd., Beijing, China, 2009.
[11] P. Fossati, L. Prencipe, G. Berti, Use of 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine, Clin. Chem., vol. 26, pp. 227–231, 1980.
[12] P. R. Santagapita, M. P. Buera, Solid Lipid Nanoparticles as Delivery Systems for Bioactive Food Components, Food Biophys. vol. 3, pp. 87–93, 2008.
[13] L. Kreilgaard, S. Frokjaer, J. M. Flink, T. W. Randolph, J. F. Carpenter, Effects of additives on the stability of Humicola lanuginosa lipase during freeze-drying and storage in the dried solid, J. Pharm. Sci., vol. 88, pp. 281–290, 1999.