Characteristics of the Storage Stability for Different Saccharomyces cerevisiae Strains
Storage stability is the important factor of baker's yeast quality. Effect of the storage period (fifteen days) on storage sugars and cell viability of baker's yeast, produced from three S. cerevisiae strains (FC-620, FH-620, and FAT-12) as comparison with baker's yeast produced by S. cerevisae F-707 (original strain of baker's yeast factory) were investigated. Studied trehalose and glycogen content ranged from 10.19 to 14.79 % and from 10.05 to 10.69 % (d.w.), respectively before storage. The trehalose and glycogen content of all strains was decreased by increasing the storage period with no significant differences between the reduction rates of trehalose. Meanwhile, reduction rates of glycogen had significant differences between different strains, where the FH-620 and FC-620 strains had lowest rates as 18.12 and 20.70 %, respectively. Also, total viable cells and gassing power of all strains were decreased by increasing the storage period. FH-620 and FC-620 strains had the lowest values of reduction rates as an indicator of storage resistant. Where the reduction rates in total viable cells of FH-620 and FC-620 strains were 22.05 and 24.70%, respectively, while the reduction rates of gassing power were 20.90 and 24.30%, in the same order. On other hand, FAT-12 strain was more sensitive to storage as compared to original strain, where the reduction rates were 35.60 and 35.75%, respectively for total viable cells and gassing power.
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 M. A. Eskarous. The Effect of Activators on the Production and Chemical Constitution of Yeast. PhD Thesis, Fac. Agric., Cairo Univ., Egypt, 1979, 176 p.
 W. Damtew. Studies on the development of baker’s yeast using cane molasses. M.Sc. Thesis, Fac. Technol., Addis Ababa Univ., Addis Ababa, 2008, 189 p.
 M. N. Rezaei, E. Dornez, P. Jacobs, A. Parsi, K. J. Verstrepen and C. M. Courtin. Harvesting yeast (Saccharomyces cerevisiae) at different physiological phases significantly affects its functionality in bread dough fermentation. Food Microbiology, 2014, vol. 39 pp. 108-115.
 H. Jorgensen, L. Olsson, B. Ronnow and E. A. Palmqvist. Fed-batch cultivation of baker’s yeast followed by nitrogen or carbon starvation: effects on fermentative capacity and content of trehalose and glycogen. Appl. Microbiol. Biotechnol., 2002, vol. 59 pp. 310-317.
 L. Plourde-Owobi, S. Durner, G. Goma and J. Francois. Trehalose reserve in Saccharomyces cerevisiae: phenomenon of transport, accumulation and role in cell viability. International Journal of Food Microbiology, 2000, vol. 55 pp. 33-40.
 S. A. Mahmud, T. Hirasawa and H. Shimizu. Differential importance of trehalose accumulation in Saccharomyces cerevisiae in response to various environmental stresses. Journal of Bioscience and Bioengineering, 2010, vol. 109 pp. 262-266.
 G. Cahill, P. K. Walsh and D. Donnelly. Determination of yeast glycogen content by individual cell spectroscopy using image analysis. Biotechnology and Bioengineering, 2000, vol. 69 pp. 312-322.
 W. A. Wilson, W. E. Hughes, W. Tomamichel and P. J. Roach. Increased glycogen storage in yeast results in less branched glycogen. Biochemical and Biophysical Research Communications, 2004, vol. 320 pp.416-423.
 M. Torija, M. Novo, A. Lemassu, W. Wilson, P. J. Roach, J. Francois and J. Parrou. Glycogen synthesis in the absence of glycogenin in the yeast Saccharomyces cerevisiae. FEBS Letters, 2005, vol. 579 pp. 3999-4004.
 A. B. Lanham, A. R. Ricardo, M. Coma, J. Fradinho, M. Carvalheira, A. Oehmen, G. Carvalho and M. A. Reis. Optimization of glycogen quantification in mixed microbial cultures. Bioresource Technology, 2012, vol. 118 pp. 518-525.
 A. M. Ismail. Chemical and Technological Studies on Baker's Yeast. MS Thesis, Fac. Agric., Al-Azhar Univ., Egypt, 2003.
 S. A. Ahmed. Microbiological and Chemical Studies on S. cerevisiae Resistant to High Concentrations of Sugar and Alcohol. PhD Thesis, Fac. Agric., Al-Azhar Univ., Egypt, 2003.
 J. S. Aranda, E. Salgado and P. Taillandier. Trehalose accumulation in Saccharomyces cerevisiae cells: experimental data and structured modeling. Biochemical Engineering Journal, 2004, vol. 17 pp. 129-140.
 A. M. Ismail. Comparison Study on Some Baker's Yeast Strains. PhD Thesis, Fac. Agric., Al-Azhar Univ., Egypt, 2006.
 M. Kus-Liskiewicz, A. Gorka and M. Gonchar. Simple assay of trehalose in industrial yeast. Food Chemistry, 2014, vol. 158 pp. 335-339.
 Y. Yoshiyama, K. Tanaka, K. Yoshiyama, M. Hibi, J. Ogawa and J. Shima. Trehalose accumulation enhances tolerance of Saccharomyces cerevisiae to acetic acid. Journal of Bioscience and Bioengineering, 2015, vol. 119 pp. 172-175.
 E. V. Soares, K. Hebbelinck and H. M. V. M. Soares. Toxic effects caused by heavy metals in the yeast Saccharomyces cerevisiae: a comparative study. Can J. Microbiol., 2003, vol. 49 pp. 336-343.
 M. Suihko and V. Mfikinen. Candida krusei in baker's yeast production. European J. Appl. Microbiol. Biotechnol., 1981, vol. 13 pp. 113-116.
 Egyptian Standard. Egyptian Standard of yeast Part 2: methods of analysis and testing for yeast. Egyptian Organization for Standardization and Quality Control, E.S. 191/2000.
 J. L. Parrou and J. Francois. A simplified procedure for a rapid and reliable assay of both glycogen and trehalose in whole yeast cells. Analytical Biochemistry, 1997, vol. 248 pp. 186-188.
 A. V. Gusakov, E. G. Kondratyeva and A. P. Sinitsyn. Comparison of two methods for assaying reducing sugars in the determination of carbohydrase activities. International Journal of Analytical Chemistry, 2011, vol. 2011 pp. 1-4.
 SAS: Statistical Analysis System, SAS / STAT User's Guide. Release 6.03 Ed. SAS Institute, Cary, NC, 1028 PP., 1999.
 J. F. Mesquita, V. M. F. Paschoalin and A. D. Panek. Modulation of trehalase activity in Saccharomyces cerevisiae by an intrinsic protein. Biochimica et Biophysica Acta, 1997, vol. 1334 pp. 233-239.
 S. H. Salem. Applying HACCP system on dried yeast production line. MS Thesis, Botany and Microbiology Dept., Fac. of Science., Al-Azhar Univ., Egypt, 2010.