Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 32009
Biosynthesis of Silver-Phosphate Nanoparticles Using the Extracellular Polymeric Substance of Sporosarcina pasteurii

Authors: Mohammadhosein Rahimi, Mohammad Raouf Hosseini, Mehran Bakhshi, Alireza Baghbanan


Silver ions (Ag+) and their compounds are consequentially toxic to microorganisms, showing biocidal effects on many species of bacteria. Silver-phosphate (or silver orthophosphate) is one of these compounds, which is famous for its antimicrobial effect and catalysis application. In the present study, a green method was presented to synthesis silver-phosphate nanoparticles using Sporosarcina pasteurii. The composition of the biosynthesized nanoparticles was identified as Ag3PO4 using X-ray Diffraction (XRD) and Energy Dispersive Spectroscopy (EDS). Also, Fourier Transform Infrared (FTIR) spectroscopy showed that Ag3PO4 nanoparticles was synthesized in the presence of biosurfactants, enzymes, and proteins. In addition, UV-Vis adsorption of the produced colloidal suspension approved the results of XRD and FTIR analyses. Finally, Transmission Electron Microscope (TEM) images indicated that the size of the nanoparticles was about 20 nm.

Keywords: Bacteria, biosynthesis, silver-phosphate, Sporosarcina pasteurii, nanoparticle.

Digital Object Identifier (DOI):

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1111


[1] Kumar, M.A., Fabrication and extraction of silver nanoparticle using bacillus thuringiensis. 2014, National Institute of Technology Rourkela.
[2] Gholami-Shabani, M., et al., Antimicrobial Activity and Physical Characterization of Silver Nanoparticles Green Synthesized Using Nitrate Reductase from Fusarium oxysporum. Applied biochemistry and biotechnology, 2014. 172(8): p. 4084-4098.
[3] Zaki, S., et al., The use of bioflocculant and bioflocculant-producing Bacillus mojavensis strain 32A to synthesize silver nanoparticles. Journal of Nanomaterials, 2014. 2014: p. 8.
[4] Sriram, M.I., K. Kalishwaralal, and S. Gurunathan, Biosynthesis of silver and gold nanoparticles using Bacillus licheniformis, in Nanoparticles in Biology and Medicine. 2012, Springer. p. 33-43.
[5] Kalishwaralal, K., et al., Biosynthesis of silver and gold nanoparticles using Brevibacterium casei. Colloids and Surfaces B: Biointerfaces, 2010. 77(2): p. 257-262.
[6] Sowani, H., et al., Green synthesis of gold and silver nanoparticles by an actinomycete Gordonia amicalis HS-11: Mechanistic aspects and biological application. Process Biochemistry, 2016. 51(3): p. 374-383.
[7] Hosseini-Abari, A., G. Emtiazi, and S.M. Ghasemi, Development of an eco-friendly approach for biogenesis of silver nanoparticles using spores of Bacillus athrophaeus. World Journal of Microbiology and Biotechnology, 2013. 29(12): p. 2359-2364.
[8] Velmurugan, P., et al., Biosynthesis of silver nanoparticles using Bacillus subtilis EWP-46 cell-free extract and evaluation of its antibacterial activity. Bioprocess and biosystems engineering, 2014. 37(8): p. 1527-1534.
[9] El-Batal, A., et al., Synthesis of silver nanoparticles by Bacillus stearothermophilus using gamma radiation and their antimicrobial activity. World Applied sciences Journal, 2013. 22(1): p. 01-16.
[10] Dhand, V., et al., Green synthesis of silver nanoparticles using Coffea arabica seed extract and its antibacterial activity. Materials Science and Engineering: C, 2016. 58: p. 36-43.
[11] Wei, X., et al., Synthesis of silver nanoparticles by solar irradiation of cell-free Bacillus amyloliquefaciens extracts and AgNO 3. Bioresource technology, 2012. 103(1): p. 273-278.
[12] Dar, M.A., A. Ingle, and M. Rai, Enhanced antimicrobial activity of silver nanoparticles synthesized by Cryphonectria sp. evaluated singly and in combination with antibiotics. Nanomedicine: Nanotechnology, Biology and Medicine, 2013. 9(1): p. 105-110.
[13] Jo, J.H., et al., Pseudomonas deceptionensis DC5-mediated synthesis of extracellular silver nanoparticles. Artificial cells, nanomedicine, and biotechnology, 2015: p. 1-6.
[14] Singh, P., et al., Weissella oryzae DC6-facilitated green synthesis of silver nanoparticles and their antimicrobial potential. Artificial cells, nanomedicine, and biotechnology, 2015: p. 1-7.
[15] Wu, A., et al., Morphology-controlled synthesis of Ag 3 PO 4 nano/microcrystals and their antibacterial properties. Materials Research Bulletin, 2013. 48(9): p. 3043-3048.
[16] Chudobova, D., et al., Comparison of the effects of silver phosphate and selenium nanoparticles on Staphylococcus aureus growth reveals potential for selenium particles to prevent infection. FEMS microbiology letters, 2014. 351(2): p. 195-201.
[17] Chen, X.-j., et al., Synthesis and characterization of Ag 3 PO 4 immobilized with graphene oxide (GO) for enhanced photocatalytic activity and stability over 2, 4-dichlorophenol under visible light irradiation. Journal of hazardous materials, 2015. 292: p. 9-18.
[18] Arul Jothi Nagarajan, S.I., Gautami Amarnath, Swathine and S.A.B.K. Chandrasekaran, Janitri V Babu, Harishankar M K, Dr. Devi A, Expeditious Synthesis of Silver Nanoparticles By A Novel Strain Sporosarcina pasteurii SRMNP1 and Patrocladogram Analysis For Exploration of its Closely Related Species. International Jounal of Scientific Research, 2014. 3(2).
[19] Williamson, G. and W. Hall, X-ray line broadening from filed aluminium and wolfram. Acta metallurgica, 1953. 1(1): p. 22-31.
[20] Uvdal, K. and T. Vikinge, Chemisorption of the dipeptide Arg-Cys on a gold surface and the selectivity of G-protein adsorption. Langmuir, 2001. 17(6): p. 2008-2012.
[21] Moreau, J.W., et al., Extracellular proteins limit the dispersal of biogenic nanoparticles. Science, 2007. 316(5831): p. 1600-1603.
[22] Banu, A., V. Rathod, and E. Ranganath, Silver nanoparticle production by Rhizopus stolonifer and its antibacterial activity against extended spectrum β-lactamase producing (ESBL) strains of Enterobacteriaceae. Materials research bulletin, 2011. 46(9): p. 1417-1423.
[23] Chen, G., et al., Facile green extracellular biosynthesis of CdS quantum dots by white rot fungus Phanerochaete chrysosporium. Colloids and Surfaces B: Biointerfaces, 2014. 117: p. 199-205.
[24] Nguyen, T.-D., C.-T. Dinh, and T.-O. Do, Monodisperse samarium and cerium orthovanadate nanocrystals and metal oxidation states on the nanocrystal surface. Langmuir, 2009. 25(18): p. 11142-11148.
[25] Hosseini, M.R. and M.N. Sarvi, Recent achievements in the microbial synthesis of semiconductor metal sulfide nanoparticles. Materials Science in Semiconductor Processing, 2015. 40: p. 293-301.
[26] Dong, C., et al., Synthesis of Ag 3 PO 4–ZnO nanorod composites with high visible-light photocatalytic activity. Catalysis Communications, 2014. 46: p. 32-35.