Physical Properties and Resistant Starch Content of Rice Flour Residues Hydrolyzed by α-Amylase
Enzymatic modification of rice flour can produce highly functional derivatives use in food industries. This study aimed to evaluate the physical properties and resistant starch content of rice flour residues hydrolyzed by α-amylase. Rice flour hydrolyzed by α-amylase (60 and 300 u/g) for 1, 24 and 48 hours were investigated. Increasing enzyme concentration and hydrolysis time resulted in decreased rice flour residue’s lightness (L*) but increased redness (a*) and yellowness (b*) of rice flour residues. The resistant starch content and peak viscosity increased when hydrolysis time increased. Pasting temperature, trough viscosity, breakdown, final viscosity, setback and peak time of the hydrolyzed flours were not significantly different (p>0.05). The morphology of native flour was smooth without observable pores and polygonal with sharp angles and edges. However, after hydrolysis, granules with a slightly rough and porous surface were observed and a rough and porous surface was increased with increasing hydrolyzed time. The X-ray diffraction patterns of native flour showed A-type configuration, which hydrolyzed flour showed almost 0% crystallinity indicated that both amorphous and crystalline structures of starch were simultaneously hydrolyzed by α-amylase.
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 N. Kavlani, V. Sharma, and L. Singh, “Various Techiques for the modification of starch and the applications of its derivatives,” Int. Res. J. Pharm., vol.3, no.5, pp. 25-31, May 2012.
 V. Olivia, E. Noemi, and A. Maria, “Physicochemical characterization of chemically modified corn starches related to rheological behavior, retrogradation and film forming capacity,”J. Food Eng., vol. 100, pp. 160-168, Sep. 2010.
 C. Rosell, and C. Collar, “Effect of various enzymes on dough rheology and bread quality,” in Recent research developments in food biotechnology. Enzymes as additives or processing aids, R. Porta, P. Di Pierro, and L. Mariniello, Eds. Kerala, India: Research Signpost, 2008, pp. 165–183.
 A. Pandey, P. Nigam, V. Soccol, D. Singh, and R. Mohan, “Advances in microbial amylases,” Biotechnol. Appl. Biochem., vol. 31, pp. 135-152, Apr.2000.
 D. Gallant, B. Bouchet, A. Buleon, and S. Perez, “Physical characteristics of starch granules and susceptibility to enzymatic degradation,”Eur. J. Clin. Nutr., vol.46, pp. 3-16, 1992.
 A. Kimura, and J.F. Robyt, “Reaction of enzymes with starch granules: enhanced reaction of glucoamylase with gelatinized starch granules,” Carbohydr. Res.,Vol. 287, no. 2, pp. 255-261, June 1996.
 M. van der Maarel, B. van der Veen, J. Uitdehaag, H. Leemhuis, and L. Dijkhuizen, “Properties and applications of starch-converting enzymes of the α-amylase family,” Biotechnology, Vol. 94, no. 2, pp. 137-155, Mar. 2002.
 J.L. Shi, J.N. Li, Q.G. Chen, L. Li, and X.Z. Hu, “Effect of amylolytic degree on characteristics of spray dried oat powder,” Trans. Chin. Soc. Agric. Mach., Aug. 2012.
 H. Englyst, S. Kingman, and J. Cummings, “Classification and measurement of nutritionally important starch fractions,” Eur. J. Clin. Nutr., Vol. 46, pp. 33-50, Oct. 1992.
 H. Zhanga, and Z. Jin, “Preparation of products rich in resistant starch from maize starch by an enzymatic method,”Carbohyd. Polym.,vol.86, no.4, pp. 1610-1614, Oct. 2011.
 P. Colonna, A. Buleon, and F. Lemarie, “Action of Bacillus subtilis -amylase on native wheat starch,” Biotechnol. Bioeng., vol. 31, pp.895-904, 1988.
 V. Komolprasert, and R. Ofoli, “Starch hydrolysis kinetics of Bacillus licheniformis α-amylase”J. Chem. Tech. Biotechnol., vol. 51, pp. 209-223, 1991.
 P. Bajpai, K. Gera, and K. Bajpai, “Optimization studies for the production of α-amylase using cheese whey medium,” Enzym. Microb. Tech., vol. 14, no. 8, pp. 679-683, Aug. 1992.
 G. R. Agarwal, K. Agarwal, and O.P. Agarwal, “Enzyme” in Text book of biochemistry, 14th ed., Meerut, India: Krishna prakashan Media, 2007, pp. 24-52.
 R. Mukerjea, G. Slocum, R. Mukerjea, and J. Robyt,“Significant differences in the activities of α-amylases in the absence and presence of polyethylene glycol assayed on eight starches solubilized by two methods,”Carbohydr. Res., vol. 341, no. 12, pp. 2049-2054, Sep. 2006.
 D. Manners, “The enzymatic degradation of starches”, J. M. V. Blanshard, and J. R. Mitchell, Eds. London: Butterworth, 1979, pp. 5-91.
 J. Jane, Y. Chen, L. Lee, A. McPherson, K. Wong, M. Radosavljevic, and T. Kasemsuwan, “Effects of amylopectin branch chain length and amylose content on the gelatinization and pasting properties of starch,” Cereal Chem., vol. 76, no. 5, pp. 629-637, Sep. 1999.
 Y. Song, and J. Jane, “Characterization of barley starches of waxy, normal and high amylose varieties,” Carbohyd. Polym., vol. 41, no. 4, pp. 365-377,Apr. 2000.
 R. R. Watson, V. Preedy, and S, Zibadi, Wheat and Rice in Disease Prevention and Health:Wheat and rice in disease prevention and health, 2014, pp. 358-369
 J. Man, Y. Yang, C. Zhang, F. Zhang, Y. Wang, M. Gu, Q. Liu, and C. Wei, “Morphology and structural characterization of high-amylose rice starch residues hydrolyzed by porcine pancreatic α-amylase,” Food hydrocolloids, vol. 31, pp. 195-203, 2013.
 American Association of Cereal Chemists. “Methods 26-50, 44-15A Approved methods of the AACC,” 10th Ed., St. Paul, MN: The Association, 2000, pp.76–21.
 O. Lopez, S. Vina, N. Pachas, M. Sisterna, P. Rohastsch, and A. Mugride, “Composition and food properties of pachyrhizus ahipa roots and starch,” Int. J. Food Sci. Tech., vol. 45, no. 2, pp. 223-233, Feb. 2010.
 N. Sodhi, and N. Singh, “Morphological, thermal and rheological properties of starches separated from rice cultivars grown in India,” Food chem., vol. 80, pp. 99-108, Jan. 2003.
 I. Goñi, L. Garcia-Diz, E. Mañas, and F. Saura-Calixto, “Analysis of resistant starch: a method for foods and food products,” Food chem., vol. 56, no. 4, pp. 445-449, Aug. 1996.
 O. Malomo, O. Ogunmoyela, S. Oluwajoba, and O. Adekoyeni, “Effect of enzymes on the quality of beer/wort developed from proportions of sorghum adjuncts,” Adv. Microbiol., vol.2, no. 4, pp. 447-451, Dec. 2012.
 C. Bamforth, “Current perspectives on the role of enzymes in brewing,” J. Cereal Sci., vol.50, no. 3, pp. 353-357, Nov. 2009.
 A. Dura, W. Błaszczak, and C. Rosell, “Functionality of porous starch obtained by amylase or amyloglucosidase treatments,” Carbohyd. Polym., vol. 101, pp. 837-845, Jan. 2014.
 C. Yook, and J. F. Robyt, “Reactions of alpha amylases with starch granules in aqueous suspension giving products in solution and in a minimum amount of water giving products inside the granule,” Carbohydr. Res., vol. 337, no. 12, pp. 1113-1117, Jun. 2002.
 Y. D. Setyawati, S. F. Ahsan, L. K. Ong, F. E. Soetaredjo, S. Ismadji, and Y. H. Ju, “Production of glutinous rice flour from broken rice via ultrasonic assisted extraction of amylose,” Food chem., vol. 203, pp. 158-164, July 2016.
 S. Hizukuri, T. Kaneko, and Y. Takeda, “Measurement of the chain legth of amylopectin and its relevance to the origin of crystalline polymorphism of starch granules,”Biochim. Biophys. Acta. Gen. Subj., vol. 760, pp. 188-191, Oct. 1983.
 J. Jane, K. Wong, and A. McPherson, “Branch-structure difference in starches of A- and B-type X-ray patterns revealed by their Naegeli dextrins,” Carbohydr. Res., vol. 300, pp. 219-227, Feb. 1997.
 M. Lauro, P. Forssell, M. Suortti, S. Hulleman, and K. Poutanen, “α-Amylolysis of large barley starch granules,” Cereal Chem., vol. 76, pp. 925-930, 1999.
 H. Leach, and T, Schoch, “Structure of the starch granule II. Action of various amylases on granular starches,” Cereal Chem., vol. 38, pp. 34-46, Jan. 1961.
 J. Li, T. Vasanthan, R. Hoover, and B. Rossnagel,“Starch from hull-less barley: V. in-vitro susceptibility of waxy, normal, and high-amylose starches towards hydrolysis by alpha-amylases and amyloglucosidase,” Food Chem., vol. 84, no. 4, pp. 621-632, Mar. 2004.
 R. Tester, J. Karkalas, and X. Qi, “Starch structure and digestibility enzymesubstrate relationship,” World’s Poultry Science Journal. 2004;60:186-95. World’s Poult. Sci. J., vol. 60, no. 2, pp. 186-195, June 2004.
 M. M. Martínez, J. Pico, and M. Gómez, “Physicochemical modification of native and extruded wheat flours by enzymatic amylolysis,” Food Chem., vol. 167, pp. 447-453, Jan. 2015.
 R. González-Soto, E. Agama-Acevedo, J. Solorza-Feria, R. Rendón-Villalobos, and L. Bello-Pérez, “Resistant starch made from banana starch by autocla-ving and debranching,” Starch/Staerke, vol. 56, no. 10, pp. 495-499, Oct. 2004.
 U. Lehmann, C. Rössler, D. Schmiedl, and G. Jacobasch, “Production and physi-cochemical characterization of resistant starch type III derived from pea starch,” Nahrung-Food, vol. 47, no. 1, pp. 60-63, Jan. 2003.
 M. Miles, V. Morris, and S. Ring, “Gelation of amylose,” Carbohydr. Res., vol. 135, no. 2, pp. 257-269, Jan. 1985.
 V. Morris, “Starch gelation and retrogradation,” Trends Food Sci. Technol., vol. 1, pp. 2-6, July 1990.
 C. Mutungi, F. Rost, C. Onyango, D. Jaros, and H. Rohm, “Crystallinity, thermal and morphological characteristics of resistant starch type III produced byhydrothermal treatment of debranched Cassava starch,” Starch/Staerke, vol. 61, no. 11, pp. 634-645, Nov. 2009.
 F. Villas-Boas, and C. Franco, “Effect of bacterial β-amylase and fungal α-amylase on the digestibility and structural characteristics of potato and arrowroot starches,” Food Hydrocolloids, vol. 52, pp. 795-803, Jan. 2016.