Commenced in January 2007
Paper Count: 30663
Chemical Characterization and Prebiotic Effect of Water-Soluble Polysaccharides from Zizyphus lotus Leaves
Abstract:In order to investigate the prebiotic potential of oligosaccharides prepared by chemical hydrolysis of water-soluble polysaccharides (WSP) from Zizyphus lotus leaves, the effect of oligosaccharides on bacterial growth was studied. The chemical composition of WSP was evaluated by colorimetric assays revealed the average values: 7.05±0.73% proteins and 86.21±0.74% carbohydrates, among them 64.81±0.42% is neutral sugar and the rest 16.25±1.62% is uronic acids. The characterization of monosaccharides was determined by high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) was found to be composed of galactose (23.95%), glucose (21.30%), rhamnose (20.28%), arabinose (9.55%), and glucuronic acid (22.95%). The effects of oligosaccharides on the growth of lactic acid bacteria were compared with those of fructooligosaccharide (RP95). The oligosaccharides concentration was 1g/L of Man, Rogosa, Sharpe broth. Bacterial growth was assessed during 2, 4.5, 6.5, 9, 12, 16 and 24 h by measuring the optical density of the cultures at 600 nm (OD600) and pH values. During fermentation, pH in broth cultures decreased from 6.7 to 5.87±0.15. The enumeration of lactic acid bacteria indicated that oligosaccharides led to a significant increase in bacteria (P≤0.05) compared to the control. The fermentative metabolism appeared to be faster on RP95 than on oligosaccharides from Zizyphus lotus leaves. Both RP95 and oligosaccharides showed clear prebiotic effects, but had differences in fermentation kinetics because of to the different degree of polymerization. This study shows the prebiotic effectiveness of oligosaccharides, and provides proof for the selection of leaves of Zizyphus lotus for use as functional food ingredients.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1110197Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2041
 H. Hammiche and K. Maiza, Traditional medicine in central sahara: pharmacopoeia of tassili n'ajjer. Journal of ethnopharmacology, vol. 105, 2006, 358-367.
 P. Ozenda, 1983- Flore du sahara. Ed. Centre national de la recherche scientifique, 1983, Paris, 617 p.
 A. Voisin. Utilisation des plantes médicinales dans le souf au 19 ème siècle, 1987, Le sahara, 1er trimestre, 100:25-28.
 D. Diallo, B. Smestad Paulsen, T. H.A Liljebäck, T. E Michaelsen. The malian medicinal plant Trichilia emetica; studies on polysaccharides with complement fixing ability. Journal of Ethnopharmacology, 2003, Vol. 84: 279-287.
 N. Saad, C. Delattre, M. Urdaci, J.M. Schmitter, P. Bressollier. An overview of the last advances in probiotic and prebiotic field. 2013, LWT - Food Science and Technology, vol. 50, pp. 1-16.
 V. Rousseau, J.P. Lepargneur, C. Roques, M. Remaud-Simeon, F. Paul. Prebiotic effects of oligosaccharides on selected vaginal lactobacilli and pathogenic microorganisms. 2005, Anaerobe, vol. 11, pp. 145–153.
 E. Biedrzycka and M. Bielecka. Prebiotic effectiveness of fructans of different degrees of polymerization. Trends in Food Science & Technology. 2004, vol. 15, pp. 170–175.
 T. S. Manning. Prebiotics. Best Practice & Research Clinical Gastroenterology. 2004, Vol. 18, No. 2, pp. 287–298.
 M.S. Madhukumar and G. Muralikrishna. Structural characterisation and determination of prebiotic activity of purified xylo-oligosaccharides obtained from Bengal gram husk (Cicer arietinum L.) and wheat bran (Triticum aestivum). 2010, Food Chemistry, vol. 118, pp. 215–223.
 A. Pompei, L. Cordisco, S. Raimondi, A. Amaretti, U. M. Pagnoni, D. Matteuzzi, M. Rossi. In vitro comparison of the prebiotic effects of two inulin-type fructans. 2008, Anaerobe, vol. 14, pp. 280–286.
 Y. Wu, S. W. Cui, J. Tang, Q. Wang, and X. Gu. Preparation, partial characterization, and bioactivity of water-soluble polysaccharides from boat-fruited sterculia seeds. Carbohydrate polymers. 2007, vol.70, pp. 437–443.
 T. R. Cipriani, C. G. Mellinger, L. M. De Souza, C. H. Baggio, C.S. Freitas, M. A. Marques, P. J. Gorin, G. L. Sassaki and M. Iacomini. Polygalacturonic acid: Another anti-ulcer polysaccharide from the medicinal plant Maytenus ilicifolia. Carbohydrate Polymers, 2009, vol. 78, pp. 361–363.
 C. S. Nergard, D. Diallo, T. E. Michaelsen, K. E. Malterud, H. Kiyohara, T. Matsumoto, H. Yamada and B. S. Paulsen. Isolation, partial characterisation and immunomodulating activities of polysaccharides from Vernoniakotschyana Sch. Bip. exWalp. Journal of Ethnopharmacology, 2004, vol. 91, pp.141–152.
 M. Dubois, K. A. Gilles, J. K.Hamilton, P. A. Rebers and F. Smith. Colorimetric method for determination of sugars and related substances. Ana. Chemistry. 1956, vol. 28, pp. 350–356.
 N. Blumenkrantz and G. Asboe-Hansen. New method for quantitative determination of uronic acids. Analytical biochemistry. 1973, vol. 54, pp 484-489.
 M. M. Bradford. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry. 1976, vol. 72, pp. 248–252.
 Zakaria Boual, Guillaume Pierre, Cedric Delattre, Fatima Benaoun, Emmanuel Petit, Christine Gardarin, Philippe Michaud, Mohamed Didi Ould El Hadj, 2015.- Mediterranean semi-arid plant Astragalus armatus as a source of bioactive galactomannan. Bioactive Carbohydrates and Dietary Fibre, 2015, vol. 5, pp. 10–18.
 J. W. Li, S. D. Ding, X. L. Ding, Optimization of the ultrasonically assisted extraction of polysaccharides from Zizyphus jujuba cv Jinsixiaozao. Journal of food engineering, 2007. vol. 80, pp. 176–183.
 D. Diallo, R. Sanogo, H. Yasambou, A. Traore, K. Coulibaly and A. Maiga, Étude des constituants des feuilles de Ziziphus mauritiana Lam. (Rhamnaceae), utilisées traditionnellement dans le traitement du diabète au Mali. Chimie, 2004, vol. 7: 1073–1080.