Commenced in January 2007
Paper Count: 31106
Synchrony between Genetic Repressilators in Sister Cells in Different Temperatures
Abstract:We used live E. coli containing synthetic genetic oscillators to study how the degree of synchrony between the genetic circuits of sister cells changes with temperature. We found that both the mean and the variability of the degree of synchrony between the fluorescence signals from sister cells are affected by temperature. Also, while most pairs of sister cells were found to be highly synchronous in each condition, the number of asynchronous pairs increased with increasing temperature, which was found to be due to disruptions in the oscillations. Finally we provide evidence that these disruptions tend to affect multiple generations as opposed to individual cells. These findings provide insight in how to design more robust synthetic circuits and in how cell division can affect their dynamics.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1107607Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1428
 J. C. Dunlap, “Molecular bases for circadian clocks,” in Cell, vol. 96, pp. 271-290, 1999.
 Z. Neubauer, and E. Calef, “Immunity phase-shift in defective lysogens: Non-mutational hereditary change of early regulation of λ prophage,” in J. Mol. Biol., vol. 51, pp. 1-13, 1970.
 R. E. Dolmetsch, K. Xu, R. S. Lewis, “Calcium oscillations increase the efficiency and specificity of gene expression,” in Nature, vol. 392, pp. 933-936, 1998.
 I. Mihalcescu, W. Hsing, and S. Leibler, “Resilient circadian oscillator revealed in individual cyanobacteria,” in Nature, vol. 430, pp. 81-85, 2004.
 D. M. Virshup, and D. B. Forger, “Keeping the beat in the rising heat,” in Cell, vol. 137, pp. 602-604, 2009.
 A. Ay, S. Knierer, A. Sperlea, J. Holland, and E. M. Ozbudak, “Short- lived Her proteins drive robust synchronized oscillations in the zebrafish segmentation clock,” in Development, vol. 140, pp. 3244-3253, 2013.
 M. B. Elowitz, and S. Leibler, “A synthetic oscillatory network of transcriptional regulators,” in Nature, vol. 403, pp. 335-338, 2000.
 T. S. Gardner, C. R. Cantor, J. J. Collins, “Construction of a genetic toggle switch in Escherichia coli,” in Nature, vol. 403, pp. 339-342, 2000.
 A. Becskei, and L. Serrano, “Engineering stability in gene networks by autoregulation,” in Nature, vol. 405, pp. 590-593, 2000.
 N. Nandagopal, and M. B. Elowitz, “Synthetic biology: Integrated gene circuits,” in Science, vol. 333, pp. 1244-1248, 2011.
 D. M. Wolf, and A. P.Arkin, "Motifs, modules and games in bacteria," in Curr. Opin. Microbiol., vol. 6, pp. 125-134, 2003.
 K. Kruse, and J. Julicher, ”Oscillations in cell biology,” in Curr. Opin. Cell Biol., vol. 17, pp. 20-26, 2005.
 J. Stricker, S. Cookson, M. R. Bennett, W. H. Mather, et al., “A fast, robust and tunable synthetic gene oscillator,” in Nature, vol. 456, pp. 516-519, 2008.
 J. G. Chandraseelan, S. M. D. Oliveira, A. Hakkinen, H. Tran, et al., “Effects of temperature on the dynamics of the LacI-TetR-CI repressilator,” in Mol. Biosyst., vol. 9, pp. 3117-3123, 2013.
 A. P. Dempster, N. M. Laird, and D. B. Rubin, “Maximum likelihood from incomplete data via the EM algorithm,” in J. Royal Stat. Soc. Ser. B (Methodological), vol. 39, pp. 1-38, 1977.