Comparison of Process Slaughtered on Beef Cattle Based on Level of Cortisol and Fourier Transform Infrared Spectroscopy (FTIR)

Authors: Pudji Astuti, C. P. C. Putro, C. M. Airin, L. Sjahfirdi, S. Widiyanto, H. Maheshwari

Abstract:

Stress of slaughter animals starting long before until at the time of process of slaughtering which cause misery and decrease of meat quality. Meanwhile, determination of animal stress using hormonal such as cortisol is expensive and less practical so that portable stress indicator for cows based on Fourier Transform Infrared Spectroscopy (FTIR) must be provided. The aims of this research are to find out the comparison process of slaughter between Rope Casting Local (RCL) and Restraining Box Method (RBM) by measuring of cortisol and wavelength in FTIR methods. Thirty two of male Ongole crossbred cattle were used in this experiment. Blood sampling was taken from jugular vein when they were rested and repeated when slaughtered. All of blood samples were centrifuged at 3000 rpm for 20 minutes to get serum, and then divided into two parts for cortisol assayed using ELISA and for measuring the wavelength using FTIR. The serum then measured at the wavelength between 4000-400 cm-1 using MB3000 FTIR. Band data absorption in wavelength of FTIR is analyzed descriptively by using FTIR Horizon MBTM. For RCL, average of serum cortisol when the animals rested were 11.47 ± 4.88 ng/mL, when the time of slaughter were 23.27 ± 7.84 ng/mL. For RBM, level of cortisol when rested animals were 13.67 ± 3.41 ng/mL and 53.47 ± 20.25 ng/mL during the slaughter. Based on student t-Test, there were significantly different between RBM and RCL methods when beef cattle were slaughtered (P<0.05), but no significantly different when animals were rested (P>0.05). Result of FTIR with the various of wavelength such as methyl group (=CH3 ) 2986cm-1, methylene (=CH2 ) 2827 cm-1, hydroxyl (- OH) 3371 cm-1, carbonyl (ketones) (C=O) 1636 cm-1, carboxyl (COO-1) 1408 cm-1, glucosa 1057 cm-1, urea 1011 cm-1have been obtained. It can be concluded that the RCL slaughtered method is better than the RBM method based on the increase of cortisol as an indicator of stress in beef cattle (P<0.05). FTIR is really possible to be used as stub of stress tool due to differentiate of resting and slaughter condition by recognizing the increase of absorption and the separation of component group at the wavelength.  

Keywords: Cows, cortisol, FTIR, RBM, RCL, stress indicator.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1107257

References:

[1] Coldizt, I.G., Watson, D.L., Kilgour, R., Ferguson, D.M., Prideaux, C., Ruby, J., Kirkland, P.D. dan Sullivan, K., 2006. Impact of animal health and welfare reserach within the CRC for Cattle and Beef Quality on Australian beef production. Australian J. ExpAgri 46, 233-244.
[2] Mounir, L., Dubroeucq, H., Andason, S. danVeissier, I., 2006. Variations in meat pH of beef bulls in relation to conditions of transfer slaugter and previous history of the animals, J. Anim Sci. 84, 1567-1576.
[3] Squires E.J., 2003. Applied Animal Endocrinology. CAB International Publishing Wallingford, Oxon 8 DE, UK.
[4] Kunitsy n V. GdanPanin L., 2013. Mechanism of Erythrocyte Deformation under the Action of Stress Hormones. Intertl. J of Biophysics 2013, 3(1): 14.
[5] Dunn, C. S., 1990. Stress reactions of cattle undergoing ritual slaughter using two methods of restraint. Vet.Rec.126:522.
[6] Minaeva VA, Minaev BF and Hovorum DM. 2008. Vibrational spectra of the Steroid Hormones, Estradiol and Estriol, Calculated by Density Functional Theory. The Role of Low-frequency Vibration, ISSN 0201- 8470.
[7] Sjahfirdi L, Aziz SN, Maheshwari H, Astuti P, Suyatna FD, Nasikin M. 2011, Estrus Period Determination of Female Rats (Rattusnorvegicus) by Fourier Transform Infrared (FTIR) Trough Identification of Reproductive Hormone Metabolites in Urine. Interl. J of Basic and Applied Sciences IJBAS-IJENS (11)(03): 158-163
[8] Smith AL.1979.Applied Infrared Spectroscopy: fundamental, technique and analytical problem-solving, A Wiley-Interscience Publication, John Wiley and Sons, New York, Brisbane and Toronto.\
[9] Suseno and Firdausi, 2008. In Sjahfirdi, L. 2011. Development of Blood Reproductive Hormone Test Using FTIR to Monitor Phases in Mammals Reproductive cycle: A preliminary study. Proc. of International Seminar of Fundamental and Application Chemical Engineering (in Press).
[10] Most E and R Palme. Hormones as Indicators of Stress. Domestic Animal Endocrinology 23: (2002) 67–74
[11] Strachan MWJ, Rebecca M. Reynolds, Riccardo E. Marioni& Jacqueline F. Price2011. Cognitive function, dementia and type 2 diabetes mellitus in the elderly. Nature Reviews Endocrinology 7, 108-114
[12] Palme R. 2012. Monitoring stress hormone metabolites as a useful, noninvasive tool for welfare assessment in farm animals. Animal Welfare 21: 331-337
[13] Ewbank, R., M.J. Parker, and C.W.Mason., 1992.Reactions of cattle to head restraint at stunning: A practical dilemma.Anim. Welfare1:55.
[14] Firgorita I., 2013. Restrain dan Penyembeli hantanpa Pemingsanan. Veterinary Educational Establishments Workshop of the OIE Improved Animal Welfare Programme (IAWP) Institut Pertanian Bogor.
[15] Whittington, P and Hewitt, L., 2009. Review of the Mark I, II and III cattle restraining boxes, project code: W.LIV.0371, Meat & Livestock Australia Ltd, North Sydney.
[16] Shaw, R. A., and Mantsch, H. H., 1997. Infrared Spectroscopy in Clinical and Diagnostic Analysis. Institute for Biodiagnostics, National Research Council of Canada, winnipeg, Canada.
[17] Kleiner,J. Ramesh, M. Huleihel, B. Cohen, K. Kantarovich, C. Levi, B. Polyak, R.S. Marks, J. Mordehai, Z. Cohen, dan S. Mordechai, 2002. A comparative study of gallstones from children andadults using FTIR spectroscopy and fluorescence microscopy, BMC Gastroenterology, 2(3), 1-14.
[18] Khaustova S, Shkurnikov M,Tonevitsky E, Artyushenkoa V and Tonevitsky A., 2010.Noninvasive biochemical monitoring of physiological stress by Fourier transform infrared saliva spectroscopy. Vol. 135, pp 3183-3192.
[19] Widiyanto S, Widiyono I, Putro PP, Astuti, P. 2014. Stress Estimation of Preslaughtered and Slaughtered by mean of Fourier Transform Infrared (FTIR) Spectroscopy Analysis Trough Measurement Cortisol and Catecholamine Level in Female Cattle Urine. Journal of The Indonesian Tropical and Agriculture (in press)
[20] SjahfirdiL, Septian A, Maheshwari H, Astuti P, Frans D, Suyatna, and Nasikin M. 2011. Determination of Estrus Period in Female Rats (Rattusnorvegicus) by Fourier Transform Infrared (FTIR) Spectroscopy Through Identification of Reproductive Homrone in Blood Samples. World Applied Sciences Journal 14(4): 539-545
[21] Guyton. 2009. Fisiologi Manusiadan Mekanisme Penyakit. Penerbit Buku Kedokteran EGC:Jakarta.
[22] Tume, R. K., dan F. D. Shaw. 1992. Beta-endorphin and cortisol concentrations in plasma of blood samples collected during exsanguination of cattle. Meat Sci.31:211.
[23] Sjahfirdi L, Mayangsari and Nasikin M. 2012. Protein Identification using Fourier Transform Infrared (FTIR)IJRRAS (10) (3) : 418-421
[24] Tabatabaei A S. 2013. Evaluate Aging Effect of SBS Modified Bitumen, World Academy of Science, Engineering and Technology (7)(2): 205- 208.