Ultrasensitive Hepatitis B Virus Detection in Blood Using Nano-Porous Silicon Oxide: Towards POC Diagnostics
Early diagnosis of infection like Hep-B virus in blood is important for low cost medical treatment. For this purpose, it is desirable to develop a point of care device which should be able to detect trace quantities of the target molecule in blood. In this paper, we report a nanoporous silicon oxide sensor which is capable of detecting down to 1fM concentration of Hep-B surface antigen in blood without the requirement of any centrifuge or pre-concentration. This has been made possible by the presence of resonant peak in the sensitivity characteristics. This peak is observed to be dependent only on the concentration of the specific antigen and not on the interfering species in blood serum. The occurrence of opposite impedance change within the pores and at the bottom of the pore is responsible for this effect. An electronic interface has also been designed to provide a display of the virus concentration.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1100737Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2238
 Li Mei, Peng Wu, Jing Ye, Guangping Gao, Lin Shao, Shaomei Huang, Yaoming Li, Xiaohong Yang, Huanchun Chen, and Shengbo Cao, “Development and application of an antigen capture ELISA assay for diagnosis of Japanese encephalitis virus in swine, human and mosquito,” Virology Journal , vol. 9: 4, 2012.
 Xue-Yong Huang, Xiao-Ning Hu, Hong Ma, Yan-Hua Du, Hong-Xia Ma, Kai Kang, Ai-Guo You, Hai-Feng Wang, Li Zhang, Hao-Min Chen, J. Stephen Dumler, and Bian-Li Xu, “Detection of New Bunyavirus RNA by Reverse Transcription–Loop-Mediated Isothermal Amplification,” J. clinical microbiology, vol. 52, 2014, pp. 531-535.
 Yong-Hwan Choi, Ga-Yeon Lee, Hyuk Ko, Young Wook Chang, Min- Jung Kang, and Jae-Chul Pyun, “Development of SPR biosensor for the detection of human hepatitis B virus using plasma-treated parylene-N film,” Biosens. Bioelectron., vol. 56, 2014, pp. 286–294.
 Cuixia Ma, Mo Liang, Li Wang, Hua Xiang, Yingtao Jiang, Yiyan Li, and Guoming Xie, “MultisHRP-DNA-coated CMWNTs as signal labels for an ultrasensitive hepatitis C virus core antigen electrochemical immunosensor,” Biosens. Bioelectron., Vol. 47, 2013, pp. 467–474.
 Jee-Yeon Kim, Jae-Hyuk Ahn, Dong-II Moon, Tae Jung Park, Sang Yup Lee, and Yang-Kyu Choi, “Multiplex electrical detection of avian influenza and human immunodeficiency virus with an underlapembedded silicon nanowire field-effect transistor,” Biosens. Bioelectron., vol. 55, 2014, pp. 162–167.
 Dawid Nidzworski, Paulina Pranszke, Magda Grudniewska, Ewelina Król, and Beata Gromadzka, “Universal biosensor for detection of influenza virus,” Biosens. Bioelectron., vol. 59, 2014, pp. 239–242.
 Thi Thanh Binh Nguyen, Guiwan Koh, Hui Si Lim, Anthony JS Chua, Mary M L Ng, and Chee Seng Toh, “Membrane-Based Electrochemical Nanobiosensor for the Detection of Virus,” Anal. Chem., vol. 81, 2009, pp. 7226–7234.
 Jiajia Deng and Chee-Seng Toh, “Impedimetric DNA Biosensor Based on a Nanoporous Alumina Membrane for the Detection of the Specific Oligonucleotide Sequence of Dengue Virus,” Sensors, vol. 13, 2013, pp. 7774-7785.
 Gengfeng Zheng, Xuan P. A. Gao, and Charles M. Lieber, “Frequency Domain Detection of Biomolecules Using Silicon Nanowire Biosensors,” NanoLetts., vol. 10, 2010, pp. 3179–3183.
 Roberto de la Rica and Molly M. Stevens, “Plasmonic ELISA for the ultrasensitive detection of disease biomarkers with the naked eye,” Nat. Nanotech., vol. 7, 2012, pp. 821–824.
 H. Ghosh and C. RoyChaudhuri, “Ultrasensitive food toxin biosensor using frequency based signals of silicon oxide nanoporous structure,” Appl. Phys. Lett., Vol. 102, 2013, pp. 243701.
 R. Dev Das, S. Maji, S. Das, and C. RoyChaudhuri, “Optimization of covalent antibody immobilization on macroporous silicon solid supports,” Appl. Surface Sci., vol. 256, 2010, pp.5867-575.