Effect of Germination on Proximate, Available Phenol and Flavonoid Content, and Antioxidant Activities of African Yam Bean (Sphenostylis stenocarpa)

Authors: Nneka N. Uchegbu, Ndidi F. Amulu

Abstract:

The work studied the effect of germination on proximate, phenol and flavonoid content and antioxidant activities (AOA) of African Yam been (AYB). Germination was done in controlled dark chamber (100% RH, 28oC). The proximate, phenol and flavonoid content and antioxidant activities before and after germination were investigated. The crude protein, moisture, and crude fiber content of germinated AYB were significantly higher (P<0.05) than that of ungermianated seed, while the fat, Ash and carbohydrate content of ungerminated were higher than the germinated seed. Germination increased the phenol and flavoniod content by 19.14% and 14.53% respectively. The results of AOA assay showed that the DPPH, reducing power and FRAP of germinated AYB seed gave high values: 48.92 ±1.22 μg/ml, 0.75± 0.15μg/ml and 98.60±0.04 μmol/g while that of ungerminated seed were: 31.33μ/ml, 0.56±1.52μg/ml and 96.11±1.13μmol/g respectively. Germinated AYB has phytochemicals with potential AOA for disease prevention.

Keywords: Antioxidant, flovonoid, germination, phenol.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1099750

References:

[1] H. Kikuzaki,M. Hisamoto, K. Hirose, K. Akiyama and H. Taniguchi, “Antioxidant properties of ferulic acid and its related compounds”, Journal of Agricultural and Food Chemistry. Vol. 50, pp. 2161 – 2168, 2002.
[2] F. Pourmorad, S.J. Hosseinimehr and N. Shahabimajd, “Antioxidant activity, phenol and flavonoid contents of some selected Iranian Medicinal plants”. Afr. J. Biotech. Vol. 5, pp. 1142 – 1145, 2006
[3] R.M. Atiqur, R.M.D. Mizanur, I.S.M. Mominul, R. Mashiar, M.S. Shabah and M.F. Alam, “ Free radical scavenging activity and phenolic content of Cassia sophera L”. Afr. J. Biotech. Vol. 7, No. 10, pp. 1591 – 1593, 2008.
[4] M. Duenas, T. Hernandez and I. Estrella, “Phenolic composition of the cotyledon and the seed coat of lentils (Lens culinaris L.).” European Food Research and Technology. No. 215, pp. 478 – 483, 2002.
[5] M.L. Lopez-Amoros, T. Hemandez and I. Estrella, “Effect of germination on legume phenolic compounds and their antioxidant activity.” J. Food Compos. Anal. No. 19, pp. 277 – 283, 2006.
[6] O.A. Ojiako, C.U. Igwe, N.C. Agha, C.A. Ogbuji and V.A. Onwuliri, “Protein and Amino Acid compositions of Sphenostylis stenocarpa, Sesamum indicum, Mondora myristica and Afzelia africana seeds from Niger.” Pakistan Journal of Nutrition. Vol. 9, No. 4, pp. 368 – 372, 2010.
[7] A. Yasmin, A. Zeb, A.W. Khalil, G.M. Paracha and A.B. Khattak, “Effect of processing on antinutritional factors of red kidney bean (Phaseolus valgaris) grains.” Food Bioprocess Technology, vol. 1, No. 4, pp. 415 – 419, 2008.
[8] K. Sowndheraraja, P. Shidduraju, and S. Manian, “Antioxidant and free radical scavenging capacity of the underutilized legumes (Vigna veillata L.)” J. of Food Compt and Analy. No. 24, pp. 160 – 165, 2011.
[9] AOAC. Association of Official Analytical Chemists. Official methods of analysis of the AOAC. Association of Official Analytical Chemists, Washington D.C., USA, 1990.
[10] H.P.S. Makkar, M. Blummel, N.K. Borowy and K. Becker, (1993). “Gravimetric determination of tannins and their correlations with chemical and protein precipitation method.” Journal of Science and Food Agriculture. No. 61, pp. 161 – 165, 1993.
[11] C. Chang, M. Yang, H. Wen and J. Chem, “Estimation of total flavonoid content in propolis by two complementary colorimetric methods.” J. Food Drug Analy., No. 10, pp. 178 – 182, 2002.
[12] M.S. Blosis, “Antioxidant determinations by the use of stable free radical nature” vol. 81, pp. 1199 – 2000, 1958.
[13] M. Oyaizu, “Studies on products of browning reaction: Antioxidant activities of products of browning reaction prepared from glucosamine.” Jap. J. Nut. Vol. 44, pp. 307 – 315, 1986.
[14] F.F. Benzie and J.J. Strain, “The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power”. The FRAP assay. Analytical Biochemistry. No. 239, pp. 70 – 79, 1996.
[15] J.N. Nwosu, “Evaluation of the proximate composition and antinutritional properties of African yam bean (Sphenostylis sternocarpa) using malting treatment.” International Journal of Basic and Applied Sciences. Vol. 2, No. 4, pp. 157 – 169, 2013.
[16] A.A. Yagoub, Muhammad and A.A. Abu-Bakar, “Effect of soaking, sprouting and cooking on chemical composition of bio availability of minerals and in vitro protein digestibility of roselle Hibiscus sadbaritta (L.). Seed.” Pak. Y. Nutr. Vol. 7, No. 1, pp. 50 – 56, 2008.
[17] J.A. Elegbede, Legumes. In: Osagie A.U. and Eka O.U. (Eds), “Nutritional Quality of plant foods. Pest Harvest Research Unit, Department of Biochemistry, University of Berlin, Benin City, Nigeria. 1988, Pp. 120 – 133.
[18] A. Doss, M. Pugalenthi, D. Rajendrakumaran and V. Vadivel, “Phenols, flavonoids and antioxidant activity of underutilized legume seeds.” Asian J. Exp. Biol. Sci. vol. 1, No. 3, 2010.
[19] Z. Zadak, R. Hromadka, M. Hronek, A. Ticha and R. Hyspler, “Functional food stuffs and diabetes-evidence and myths.” Vnitr Lek vol. 55, No. 4, pp. 412 – 415, 2009.
[20] M. Duenas, J. Hernandez, I. Estrella and D. Fernandez, “Germination as a process to increase the polyphenol content and antioxidant activity of Lupin seeds (Lupines angustifolius L.)” Food Chem. No. 177, pp. 599 – 607, 2009.
[21] F. Shahidi and M. Naczk, “Phenolic compounds in grains. In: food phenolics; source, Chemistry Effect Applications”, Technomic Publishing Company Inc., Lancaster P.A., 1995, pp. 3 – 39.
[22] R. Randhr, Y.T. Lin and K. Shetty, “Stimulation of phenolics, antioxidant and antimicrobial activities in dark germinated mung bean sprouts in response to peptide and phytochemical elicitors.” Process Biochem. Vol. 39, pp. 637 – 647, 2004.
[23] C. Sanchez-Moreno, “Methods used to evaluate the free radical scavenging activity on foods and biological systems.” Food Sci. Tech. Int. Vol. 8, pp. 121 – 137, 2002.
[24] Y. Zou, Y. Lu and D. Wei, “Antioxidant activity of a flavonoid-rich extract of Hypericum perforatum L.” In vitro. Journal of Agricultural and Food Chemistry. Vol. 52, pp. 5032 – 5039, 2004.
[25] L. Yu, S. Haley, J. Perret, M. Harris, J. Wilson and M. Qian, “Free radical scavenging properties of wheat extracts.” J. Agricult. Food Chem. Vol. 50, pp. 1619 – 1624, 2002.
[26] H.M. Dorman, K. Kosar, Y. Kahlos, Holm, R. Hiltunen, “Antioxidant properties and composition of aqueous extracts from mentha species, hybrids, varieties, and cultivars.” J. Agri. and Food Chem. Vol. 51, pp 4563 – 4569, 2003.
[27] R. Fernandez-Orozc, J. Frias, H. Zielenski, R. Munoz, K. Piskula, H. Kozlowska, C. Vidal-Vulverde, “Evaluation of bioprocesses to improve the antioxidant properties of chickpeas.” LWT – Food Sci. Technol. Vol. 42, pp 885 – 895, 2009.