Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 31584
In vitro Antioxidant Activity of Two Selected Herbal Medicines

Authors: S. Vinotha, I. Thabrew, S. Sri Ranjani


Hot aqueous and methanol extracts of the two selected herbal medicines such are Vellarugu chooranam (V.C) and Amukkirai Chooranam (A.C) were examined for total phenolic and flavonoid contents and in vitro antioxidant activity using four different methods. The total phenolic and flavonoid contents in methanol extract of V.C were found to be higher (44.41±1.26mg GAE/g; 174.44±9.32mg QE/g) than in the methanol extract of A.C (20.56±0.67mg GAE/g; 7.21±0.85mg QE/g). Hot methanol and aqueous extracts of both medicines showed low antioxidant activity in DPPH, ABTS, and FRAP methods and Iron chelating activity not found at highest possible concentration. V.C contains higher concentrations of total phenolic and flavonoid contents than A.C and can also exert greater antioxidant activity than A.C, although the activities demonstrated were lower than the positive control Trolox. The in vitro antioxidant activity was not related with the total phenolic and flavonoid contents of the methanol and aqueous extracts of both herbal medicines (A.C and V.C).

Keywords: Activity, Different extracts, Herbal medicine, in vitro antioxidant.

Digital Object Identifier (DOI):

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2268


[1] M. A. Ebrahimzadeh, F. Pourmorad and A. R. Bekhradnia, “Iron chelating activity, Phenol and flavonoid content of some medicinal plants from Iran”, African Journal of Biotechnology, vol. 7 (18), 17 September 2008, pp. 3188-3192.
[2] S. Panchawat, “In vitro Free Radical Scavenging Activity of Leaves Extracts of Withania somnifera” Recent Research in Science and Technology RRST-Pharmacology, vol.3 (11), 2011, pp. 40-43.
[3] N. R .Bhalodia, P. B. Nariya, R. N. Acharya, and V. J. Shukla, “Evaluation of in vitro Antioxidant activity of Flowers of Cassia fistula Linn.”, International Journal of PharmTech Research, vol. 3, No.1, Jan- Mar 2011; pp 589-599.
[4] P. K. Jain and R. K. Agrawal, “Antioxidant and Free Radical Scavenging Properties of Developed Mono - and Polyherbal Formulations”, Asian J. Exp. Sci., vol. 22, No. 3, 2008, pp. 213-220.
[5] K. S. Murukesu muthaliyar, “Gunapadam I (Vegetable kingdom)”, Siddha maruthuva varrium, Chennai 600106, 1936, pp. 1, 606-60.
[6] S. Kannusamipillai, “Siddha Vaidthiya Patharththa Guna Vilakam, (Moola varkkam)”, B. Rathinanajakar & Sons, Chennai, 1967 & 1998, pp. 6, 09-671.
[7] V. L. Singleton, R. Orthofer, and R. M. Lamuela-Raventos, “Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocaltue reagent”, Meth Enzymol, 299, 1999, pp. 152-178.
[8] A. V. Badarinath, K. Mallikarjuna RAo, C. M. S. Chetty, S. Ramkanth, T. V. S. Rajan, and K. Gnanaprakash, “A Review on in vitro Antioxidant Methods: Comparisions, Correlations and Considerations”, International Journal of Pharm Tech Research, vol. 2. no.2, 2010, pp. 1276-85.
[9] P. Carter, “Spectrophotometric determination of serum iron at the submicrogram level with a new reagent ferrozine”, Annual Biochemistry, 40, 1971, pp. 450-58.
[10] M. S. Blois, “Antioxidant determination by use of stable free radical”, Nature, 181, 1958, pp. 1199-1200.
[11] I. F. F. Benzine, and Y. T. Szeto, “Total antioxidant capacity of teas by the ferric reducing antioxidant power assay”, J. Agric. Food Chem., 47, 1999, pp. 633-36.
[12] R. Re, N. Pellegrini, A. Proteggente, A. Pannala, and M. Yang, “Antioxidant activity applying an improved ABTS radical cation decolorization Assay”, Free Radical Biol. Med, 26, 1999, pp. 1231-37.
[13] I. Hinneburg, H. J. D. Dorman, and R. Hiltunen, “Antioxidant activities of extracts from selected culinary herbs and spices”, Food Chemistry 97 2006, pp. 122–129.
[14] S. M. Nabavi, M. A. Ebrahimzadeli, S. F. Nabavi, A. Hamidina and A. R. Bekhradnia, “Determination of antioxidant activity, phenol and flavonoid content of Parrota Persia MEY”, Pharmacology online, 2: 2008, pp. 560-567.
[15] S. A. Yadava, L. F. Hakkim, and R. Sathishkumar, “Antioxidant activity of Withania somnifera (L) Dunal by different solvent extraction methods”, Journal of Pharmacy Research, vol. 4 (5), 2011, pp. 1428- 1430.
[16] M. Kessler, G.C. Ubeaud and L. Jung, “Anti- and pro-oxidant activity of rutin and quercetin derivatives”, J. Pharm. Pharmacol. 53, 2003, pp. 131-142.
[17] A. Prakash, F. Rigelhof and E. Miller, (Dr. Jonathan DeVries, Editor), “Antioxidant Activity”, Medallion Laboratories Analytical Progress,