The Co-application of Plant Growth Promoting Rhizobacteria and Inoculation with Rhizobium Bacteria on Grain Yield and Its Components of Mungbean (Vigna radiate L.) in Ilam Province, Iran

Authors: Abdollah Hosseini, Abbas Maleki, Khalil Fasihi, Rahim Naseri

Abstract:

In order to investigate the effect of Plant Growth Promoting Rhizobacteria (PGPR) and rhizobium bacteria on grain yield and some agronomic traits of mungbean (Vigna radiate L.), an experiment was carried out based on randomized complete block design with three replications in Malekshahi, Ilam province, Iran during 2012-2013 cropping season. Experimental treatments consisted of control treatment, inoculation with rhizobium bacteria, rhizobium bacteria and Azotobacter, rhizobium bacteria and Azospirillum, rhizobium bacteria and Pseudomonas, rhizobium bacteria, Azotobacter and Azospirillum, rhizobium bacteria, Azotobacter and Pseudomonas, rhizobium bacteria, Azospirillum and Pseudomonas and rhizobium bacteria, Azotobacter, Azospirillum and Pseudomonas. The results showed that the effect of PGPR and rhizobium bacteria were significant affect on grain and its components in mungbean plant. Grain yield significantly increased by PGPR and rhizobium bacteria, so that the maximum grain yield was obtained from rhizobium bacteria + Azospirillum + Pseudomonas with the amount of 2287 kg.ha-1 as compared to control treatment. Excessive application of chemical fertilizers causes environmental and economic problems. That is, the overfertilization of P and N leads to pollution due to soil erosion and runoff water, so the use of PGPR and rhizobium bacteria can be justified due to reduce input costs, increase in grain yield and environmental friendly.

Keywords: Azotobacter, Mungbean, Pseudomonas, Rhizobium bacteria.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1096529

References:

[1] Bagheri, A. Zand and M. Parsa,"Beans”, the bottlenecks and strategies of jihad Mashhad University Press, pp. 56-86. 1998.
[2] N. Majnoon Hosseini,"Agriculture and grain production”, Fourth edition. Jihad Publication of Tehran University, Iran. 2008.
[3] A. Fenwick Kelly and A. Ray mond,"Encyclopaedia of seed production of world crops”, John wiley and New England, pp. 403. 1998.
[4] A. Wani, M. Saghir Khanand A. Zaidi," Synergistic effects of the inoculation with nitrogen-fixing and phosphate-solubilizing rhizobacteria on the performance of field-grown chickpea”, Plant Nutr. Soil Sci. 170, 283–287. 2007
[5] P. Gyaneshwar, K.G. Naresh and L. J Parekh,"Effect of buffering on the phosphate solubilizing ability of microorganisms”, World J. Microbiol. Biotechnol. 14, 669–673. 1998.
[6] P. Ponmurugan and C. Gopi," In vitro production of growth regulators and phosphatase activity by phosphate solubilizing bacteria”, African J. Biotechnol. 5, 340–350. 2006
[7] S. Samman, J.W.Y. Chow. M.J. Foster, Z.I. Ahmad, J.L. Phuyal and P. Petocz,"Fatty acid composition of edible oils derived from certified organic and conventional agricultural methods”, Food Chemistry. 109: 670-674. 2008.
[8] Zahir, A. Z. M. Arshad, and W. F. Frankenberger,"Plant growth promoting rhizobacteria: applications and perspectives in agriculture”, Advances in Agronomy. 81: 97-168. 2004.
[9] S.K. Sarawgi, P.K. Tiwari and R.S. Tripathi," Uptake and balance sheet of nitrogen and phosphorus in gram (Cicer arietinum) as influenced by phosphorus , biofertilizers and micronutrients under rainfed conditions”, Ind. J. Agron. 44, 768–772. 1999.
[10] A.E. Richardson, P.A. Hadobas, J.E. Hayes, C.P. O’Hara and R.J. Simpson, "Utilization of phosphorus by pasture plants supplied with myco-inositol hexaphosphate is enhanced by the presence of soil microorganisms”, Plant Soil. 229, 47–56. 2001.
[11] A .Zaidi and M.S. Khan, "Interactive effect of rhizospheric microorganisms on growth, yield and nutrient uptake of wheat”, J. Plant Nutr. 28, 2079–2092. 2005.
[12] R.M.N. Kucey, H.H. Janzen and M.E. Legget, "Microbial mediated increases in plant available phosphorus”, Adv. Agron. 42, 199–228. 1989.
[13] Kennedy, I. R., Choudhury, A. T. A. M., and M.L. Kecskes,"Nonsymbiotic bacterial daizotrophs in crop-farming systems”, can their potential for plant. 2004.
[14] Sayadi, A. Pouraboghadare and M.J. Zare,"Effect of seed pretreatment with hormone 2, 4-D and Azospirillum on bean yield”, 12th Iranian Crop Science Congress. Islamic Azad University Karj Branch. 2011.
[15] A. Hamidi, and A. Asgharzadeh,"Study of the Effect of Plant Growth Promoting Rhizobacteria in maize cropping. Iranian J. Soil and Water. 20 (2): 133-134. 2006.
[16] A. Zahir, Z.M. Arshad and W.F. Frankenberger,"Plant Growth Promoting Rhizobacteria applications and perspectives in agriculture”, Advances in Agronomy, 81: 97-168. 2004.
[17] S. Marius, A. Octavita, U. Eugen and A. Vlad, "Study of a microbial inoculation on several biochemical indices in sunflower (Helianthus annuus L.) ”, 2005.
[18] F.Y. Hafeez, M.E. Safdar, A.U. Chaudry and K.A. Malik ,"Rhizobial inoculation improves seeding emergence, Nutrient uptake and growth of cotton. Australian Journal of Experimental. And M. Heydrich. 1995. Selection of Rhizobacteria for Use in maize Cultivation”, Cultivos Tropicales, 6: 5-8. 2004.
[19] Rohitashav-Singh, Sood, B.K., V.K. Sharma and R. Singh, "Response of forage maize (Zea mays L.) to Azotobacter inoculation and nitrogen”, Indian J. Agronomy, 38: 555-558. 1993.
[20] A.N. Hernandez, A. Hernandez and M. Heydrich,"Selection of rhizobacteria for use in maize cultivation. Cultivos Tropicales, 6: 5-8. 1995.
[21] K.V.B.R. Tilak, C.S. Singh, V.K. Roy and N.S.S. Rao,"Azospirillum brasilense and Azotobacter chroococcum Inoculum: Effect on yield of maize (Zea mays L.) and sorghum (Sorghum bicolor) ”, Soil Biology and Biochemistry, 14: 417- 418. 1982.
[22] M.S. Dardanelli, F.J. Fernandez de Cordoba, M. Rosario Espuny, M.A. Rodriuguez Carvajal, M.E. Soria Diaz, A.M. Gil Serrano, Y. Okon and M. Megias,"Effect of Azospirillum brasilense coinoculated with Rhizobium on Phaseolus vulgaris flavonoids and Nod factor production under salt stress”, Soil Biology and Biochemistry. 40: 2713–2721. 2008.
[23] R. Naseri and A. Mirzaei,"Response of yield and yield components of safflower (Carthamus tinctorius L.) to seed inoculation with Azotobacter and Azospirillum and different nitrogen levels under dry land conditions”, American-Eurasian J. Agric. & Environ. Sci. 9 (4): 445- 449. 2010.
[24] R. Naseri, A. Maleki, H. Naserirad, S. Shebibi and A, Omidian, " Effect of plant growth promoting rhizobacteria (PGPR) on reduction nitrogen fertilizer application in rapeseed (Brassica napus L.) ”, Middle-East Journal of Scientific Research. 14 (2): 213-220, 2013.
[25] A. Shokuh Far, R. Shoholi and Gh. Ghodrati,"Evaluate soybean response to different doses and strains of the bacterium Bradyrhizobium japonicum in the northern region of Khuzestan”, Iranian Journal of Agronomy. 4 (2): 81-92. 2008.
[26] S. Cheraghi, M. Rafiee and A. Khorgami,"Effect of foliar nitrogen, planting and residue management on yield and yield components of mungbean plants in the environment Khorramabad”, Journal of Research Crop Physiology. 9: 15-30. 2011.
[27] T. Kato,"Genetic environmental variations and association of the characters related to the grain filling processing rice cultivars. Plant product. Sci. 2(1): 32-36. 1999.
[28] S.L. Kumari and G. Valarmathi,"Relationship between grain yield grain filling rate and duration of grain filling in rice”, Madras Agric. J. 85: 210-211. 1998.
[29] R. Singh, R.K. Behl, K.P. Singh, P. Jain and N. Narula," Performance and gene effects for wheat yield under inoculation of arbuscular mycorrhiza fungi and Azotobacter chroococcum”, Haryana Agricultural University. Hisar, India. Plant Soil Environ. 50: 409-415. 2004.
[30] A .Zaidi and M.S. Khan, "Interactive effect of rhizospheric microorganisms on growth, yield and nutrient uptake of wheat”, J. Plant Nutr. 28, 2079–2092. 2005.
[31] A.Z Zahir, S.A. Abbas, A. Khalid and M. Arshad,"Substrate dependnd microbially derived plant hormones for improving growth of maize seedlings”, Pakistan Journal of Biological Science. 3: 289. 2000.
[32] R. Maliha, S. Khalil, N. Ayub, S .Alam and F. Latif,"Organic acids production and phosphate solubilization by phosphate solubilizing microorganisms (PSM) under in vitro conditions”, Pak. J. Biol. Sci. 7, 187–196. 2004.
[33] E. Hasanzadeh, D. Mazaheri, M.R. Chaichi and K. Khavazi,"Efficiency of phosphorus solubilizing bacteria and phosphorus chemical fertilizer on yield and yield components of barley cultivar Karoon Dar Kavir) ”, Pajouhesh and Sazandegi 77: 111-118. 2007.