Attenuation of Pancreatic Histology, Hematology and Biochemical Parameters in Type 2 Diabetic Rats Treated with Azadirachta excelsa
Azadirachta excelsa or locally known as sentang are frequently used as a traditional medicine by diabetes patients in Malaysia. However, less attention has been given to their toxicity effect. Thus, the study is an attempt to examine the protective effect of A. excelsa on the pancreas and to determine possible toxicity mediated by the extract. Diabetes was induced experimentally in rats by high-fat-diet for 16 weeks followed by intraperitoneal injection of streptozotocin at dosage of 35 mg/kg of body weight. Declination of the fasting blood glucose level was observed after continuous administration of A. excelsa for 14 days twice daily. This is due to the refining structure of the pancreas. However, surprisingly, the plant extract reduced the leukocytes, erythrocytes, hemoglobin, MCHC and lymphocytes. In addition, the rat treated with the plant extract exhibited increment in AST and eosinocytes level. Overall, the finding shows that A. excelsa possesses antidiabetic activity by improving the structure of pancreatic islet of Langerhans but involved in ameliorating of hematology and biochemical parameters.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1096319Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1903
 World Health Organization (2013). Key facts. In Diabetes. Retrieved March 20, 2013 from http://www.who.int/mediacentre/factsheets/ fs312/en/
 Diabetes Malaysia Association (2011). Profile of the diagnosed. In What is diabetes. Retrieved March 20, 2013 from http://www.diabetes.org.my/ article.php?aid=5
 S. Nurdiana, K. Mohamad Shukri, J. Elizabeth Jega and S. Nurul ‘Izzati, "Lowering blood glucose effect of Azadirachta excelsa leaves extract” Natural Products: An Indian Journal, vol, 9, pp. 363-366, 2013.
 A. Zainah, I. Amin, K. Shafii, M. M. Muhammad Hanaffi and H. Muhajir, "Antihyperglycemic activity of F. deltoidea ethanolic extract in normal rats” Sains Malaysiana, vol, 40, pp. 489-495, 2011.
 J. Klemens, "Herbs that lower blood sugar” The Journal for the American Association of Integrative Medicine, vol, 3, pp. 1-5, 2006.
 M. Mahsa, E. Mahdi, Y. M. Goh, H. O. Hemn, B. Mohd Hair and M. Suhaila, "Effects of brown seaweed (Sargassum polycystum) ectracts on kidney, liver, and pancreas of type II diabetic rat models (Periodical style—Accepted for publication),” IEEE Trans. Antennas Propagat., submitted for publication.
 O. Erejuwa, S. Siti Amrah, A. W. Mohd Suhaimi, N. S. Kuttulebbai, M. S. Salzihan and G. Sunil, "Antioxidant protective effect of glibenclamide and metformin in combination with honey in pancreas of streptozotocin-induced diabetic rats” Journal of Molecular Sciences, vol, 11, pp. 2056-2066, 2010.
 D. Gomathi, G. Ravikumar, M. Kalaiselvi, K. Devaki and C. Uma, "Efficacy of Evolvulus alsinoides (L.) L. on insulin and antioxidants activity in pancreas of streptozotocin induced diabetic rats” Journal of Diabetes & Metabolic Disorders, vol, 3, pp.1-6, 2013.
 M. Abdullohi, A. B. Zuki, Y. M. Goh, A. Rezaeizadeh and M. M. Noordin, "The effects of Momordica charantia on the liver in streprozotocin-induced diabetes in neonatal rats” African Journal of Biotechnology, vol, 9, pp.5004-5012, 2010.
 O. P. Ajagbonna, K. I. Onifade and U. Suleiman, "Haematological and biochemical changes in rats given extract of calathopis procera” Sokoto J Vet Sci., vol, 1, pp. 36-42, 1999.
 M. T. Yakubu, M. A. Akanji and A. T. Oladiji, "Haematological evaluation in male albino rats following chronic administration of aqueous extract of Fadogia agrestis stem” Pharmacog Meg., vol, 3, pp. 34-38, 2007.
 S. Sen, M. Kar, A. Roy and A. S. Chakraborti, "Effect of nonenzymatic glycation on function and structural properties of hemoglobin” Biophys Chem. vol. 113, pp. 289-298, 2005.
 A. A. Abolfathi, D. Mohajeri, A. Rezaie and M. Nazeri "Protective Effects of Green Tea Extract against Hepatic Tissue Injury in Streptozotocin-Induced Diabetic Rats” Evidence-Based Complementary and Alternative Medicine, vol, 2012, pp.1-10, 2011
 K.R. Senthil., M. Ponmozhi, P. Viswanathan and N. Nalini, "Activity of Cassia auriculata leaf extract in rats with alcoholic liver injury” J. Nutr. Biochem. vol. 14, pp. 452–458, 2003.