Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 31100
Cytotoxic Effects of Engineered Nanoparticles in Human Mesenchymal Stem Cells

Authors: Ali A. Alshatwi, Vaiyapuri S. Periasamy, Jegan Athinarayanan


Engineered nanoparticles’ usage rapidly increased in various applications in the last decade due to their unusual properties. However, there is an ever increasing concern to understand their toxicological effect in human health. Particularly, metal and metal oxide nanoparticles have been used in various sectors including biomedical, food and agriculture. But their impact on human health is yet to be fully understood. In this present investigation, we assessed the toxic effect of engineered nanoparticles (ENPs) including Ag, MgO and Co3O4 nanoparticles (NPs) on human mesenchymal stem cells (hMSC) adopting cell viability and cellular morphological changes as tools The results suggested that silver NPs are more toxic than MgO and Co3O4NPs. The ENPs induced cytotoxicity and nuclear morphological changes in hMSC depending on dose. The cell viability decreases with increase in concentration of ENPs. The cellular morphology studies revealed that ENPs damaged the cells. These preliminary findings have implications for the use of these nanoparticles in food industry with systematic regulations.

Keywords: Silver, MgO, cobalt oxide, human mesenchymal stem cells

Digital Object Identifier (DOI):

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2094


[1] Kessler, R., "Engineered Nanoparticles in Consumer Products Understanding a New Ingredient”. Environ. Health Persp. 2011; 119, A120–A125
[2] Jones, C.F., Grainger, D.W., "In vitro assessments of nanomaterial toxicity”. Adv. Drug Delivery Rev. 2009; 61, 438–456.
[3] Park, B., "Current and Future Applications of Nanotechnology, in Nanotechnology: Consequences for Human Health and the Environment, R.M. Harrison, R.E. Hester (Eds.), The Royal Society of Chemistry. 2007.
[4] Cientifica report, "Nanotechnologies in the food industry”. 2006; Available at:
[5] Wiechers, J.W., and Musee, N., "Engineered Inorganic Nanoparticles and Cosmetics: Facts, Issues, Knowledge Gaps and Challenges”. J. Biomed. Nanotech. 2010; 6, 408–431
[6] Chaudhry, Q., Scotter, M., Blackburn, J., Ross, B., Boxall, A., Castle, L., Aitken, R., Watkins, R., "Applications and implications of nanotechnologies for the food sector”. Food Addit. Contam. 2008; 25, 241–258.
[7] Colvin, V.L., "The potential environment impact of engineered nanomaterials”. Nat. biotechnol. 2003; 21, 1166 – 1170.
[8] Lima, Renata, Amedea B. Seabra, and Nelson Durán. "Silver nanoparticles: a brief review of cytotoxicity and genotoxicity of chemically and biogenically synthesized nanoparticles." Journal of Applied Toxicology 32, no. 11 (2012): 867-879.
[9] Lison D, Carbonnelle P, Mollo L, Lauwerys R, Fubini B, "Physicochemical mechanism of the interaction between cobalt metal and carbide particles to generate toxic activated oxygen species”, Chem Res Toxicol. 1995 Jun; 8(4):600-6.
[10] Ge, S., G. Wang, Y. Shen, Q. Zhang, D. Jia, H. Wang, Q. Dong, and T. Yin. "Cytotoxic effects of MgO nanoparticles on human umbilical vein endothelial cells in vitro." IET nanobiotechnology 5, no. 2 (2011): 36- 40.
[11] Athinarayanan, Jegan, Vaiyapuri Subbarayan Periasamy, Mohammed A. Alsaif, Abdulrahman A. Al-Warthan, and Ali A. Alshatwi. "Presence of nanosilica (E551) in commercial food products: TNF-mediated oxidative stress and altered cell cycle progression in human lung fibroblast cells." Cell biology and toxicology 30, no. 2 (2014): 89-100.
[12] Kroemer, Guido, Lorenzo Galluzzi, and Catherine Brenner. "Mitochondrial membrane permeabilization in cell death." Physiological reviews 87, no. 1 (2007): 99-163.