Using Submerge Fermentation Method to Production of Extracellular Lipase by Aspergillus niger

Authors: Masoumeh Ghasemi, Afshin Farahbakhsh, Arman Farahbakhsh, Ali Asghar Safari

Abstract:

In this study, lipase production has been investigated using submerge fermentation by Aspergillus niger in Kilka fish oil as main substrate. The Taguchi method with an L9 orthogonal array design was used to investigate the effect of parameters and their levels on lipase productivity. The optimum conditions for Kilka fish oil concentration, incubation temperature and pH were obtained 3 gr./ml 35°C and 7, respectively. The amount of lipase activity in optimum condition was obtained 4.59IU/ml. By comparing this amount with the amount of productivity in the olive oil medium based on the cost of each medium, it was that using Kilka fish oil is 84% economical. Therefore Kilka fish oil can be used as an economical and suitable substrate in the lipase production and industrial usages.

Keywords: Lipase, Aspergillus niger, Kilka Fish oil, Submerge Fermentation method.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1096039

References:

[1] H. Mala and et al.. "Mixed substrate solid state fermentation for production and extraction of lipase from Aspergillusniger,MTCC 2594”. J. Gen. Appl. Microbiol., 53, 2007,pp. 247–253.
[2] M.Sharma, "Aqueous two phase extraction – of Lipase from Rice Bran”, Advances in Applied Science Research, 2, 2, 2011, pp. 265-271.
[3] R.Gupta, N.Gupta and R.Rathi, "Bactrial lipase: an overview of production, purification and biochemical properties”,Applied Microbial Biotechnology. 64, 6, 2004, pp. 763–781.
[4] A.Kumar and S. Kanwarm, "Lipase production in Solid-State Fermentation (SSF): Recent Developments and Biotechnological Applications”, Dynamic Biotechnology, Process Biotechnology and Molecular biology., 6,2012, pp. 3-27.
[5] S.Basheer and et al., "Lipase from marine Aspergillusawamori BTMFW032: Production, partial purification and application in oil effluent treatment”, New Biotechnology, 28, 2011, pp. 627-638.
[6] A.Salihua and et al., "Lipase production: An insight in the utilization of renewable agricultural residues”,Resources, Conservation and Recycling 58, 2012, pp.36– 44.
[7] K.Adinarayana and et al., "Optimization of process parameters for production of lipase in solid-state fermentation by newly isolated Aspergillus species”Indian Journal of biotechnology,2004, pp. 65-69.
[8] N.D.Mahadik, U.S. Puntambekar, K.B. Bastawde, J.M. Khire and D.V. Gokhale, "Production of acidic lipase by a mutant of Aspergillus niger NCIM 1207 in submerged”,fermentation Process Biochemistry, 39, 2004, pp. 2031-2034.
[9] G.Falony, J. C.Armas, J. C. D.Mendoza, J. L. M.Hernández, "Production of extracellular lipase from Aspergillusniger by solid-state fermentation”, Food Technol. Biotechnol, 44, 2006, pp. 235-240.
[10] N.D.Mahadik, U.S. Puntambekar, K.B. Bastawde, J.M. Khire and D.V. Gokhale, "Production of acidic lipase by Aspergillusnigerin solid state fermentation”, Process Biochemistry, 38, 5, 2002, pp. 715-721.
[11] T. Treichel and et al. "A Review on Microbial Lipases Production”, Food Bioprocess Technology, 3,2010, pp.182–196.
[12] V. Mohanasrinivasan and et al., "A comparative study of the lipase yield by solid state and submerged fermentations using fungal speciesfrom biopharmaceutical oil waste”,African Journal of Biotechnology, 8, 1, 2009, pp. 073-076.
[13] Zh.Khoshkhoo And et al.,"Study on Changes of Protein and Lipid of Fish Protein Concentrate (FPC) Produced form Kilkas in VP and MAP Packages at Light and Darkness condition During Six Months”, International Journal of Environmental Science and Development,1, 1, 2010, pp. 101-106.
[14] R. K.Roy, "Design of Experiments Using the Taguchi Approach”, 2001.
[15] M.Hosseinpour and et al., "Submerge Culture Studied for lipase Production by Aspergillusniger NCIM 584 on soya flour”, Middle-East Journal of Scientific Research, 7, 3, 2011, pp. 362-366.
[16] T.L.F.Pinheiro, "Produção de lipases porfermentaçãoemestadosólido e fermentaçãosubmersautilizandoPenicilliumverrucosumcomomicrorganis mo”, Erechim, RS, Brasil,2006, pp.45-47.
[17] M.L.Pera and et al., "Catalytic Properties of Lipase Extracts from Aspergillus niger”, Food Technol. Biotechnol, 44, 2, 2006, pp.247–252