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Phylogenetic Characterization of Atrazine-Degrading Bacteria Isolated from Agricultural Soil in Eastern Thailand

Authors: Sawangjit Sopid


In this study sugarcane field soils with a long history of atrazine application in Chachoengsao and Chonburi provinces have been explored for their potential of atrazine biodegradation. For the atrazine degrading bacteria isolation, the soils used in this study named ACS and ACB were inoculated in MS-medium containing atrazine. Six short rod and gram-negative bacterial isolates, which were able to use this herbicide as a sole source of nitrogen, were isolated and named as ACS1, ACB1, ACB3, ACB4, ACB5 and ACB6. From the 16S rDNA nucleotide sequence analysis, the isolated bacteria ACS1 and ACB4 were identified as Rhizobium sp. with 89.1-98.7% nucleotide identity, ACB1 and ACB5 were identified as Stenotrophomonas sp. with 91.0-92.8% nucleotide identity, whereas ACB3 and ACB6 were Klebsiella sp. with 97.4-97.8% nucleotide identity.

Keywords: Atrazine-degrading bacteria, bioremediation, Thai isolate bacteria.

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[1] Y. Zhang, Z. Ning, J. Zhao, P. Xinran, M. Shuyan, and H. Miao, "Isolation of two atrazine-degrading strains and their degradation characteristics,” Int. J. Agric & Biol. Eng., vol. 2, no. 3, pp. 27–32, Sep. 2009.
[2] A. S. Azevedo, R. S. Kanwar, and L. S. Pereira, "Atrazine Transport in Irrigated Heavy- and Coarse-Textured Soils, Part I: Field Studies,” J. Agri. Eng. Res., vol. 8, no. 6, pp. 619-623, 2004.
[3] Y.-K. Kim, "Adsorption, Desorption and Movement of Napropamide in Soils,” KSCE J. Civil Eng., vol. 76, no. 2, pp. 165–174, 2009.
[4] W. W. Mulbry, H. Zhu, S. M. Nour, and E. Topp, "The triazine hydrolase gene trzN from Nocardioides sp. strain C190: cloning and construction of gene-specific primers,” FEMS. Microbiol. Lett., vol. 206, pp. 75–79, 2002.
[5] K. R. Solomon, D. B. Baker, R. P. Richards, K.R. Dixon, S.J. Klaine, T. W. La Point, R. J. Kendall, C. P. Weisskopf, J. M. Giddings, J. P. Giesy, L. W. Hall, and W. M. Williams, "Ecological risk assessment of atrazine in North American surface waters,” Environ. Toxicol. Chem., vol. 15, pp. 31–76, 1996.
[6] S. K. Widmer, and R. F. Spalding, "A natural gradient transport study of selected herbicides,” J Environ. Qual., vol. 24, pp. 445–453, 1995.
[7] E. Topp, H. Zhu, and S. M. Nour, "Characterization of an atrazine-degrading Pseudaminobacter sp. isolated from Canadian and French agricultural soils,” Appl. Environ. Microbiol., vol. 66, no. 7, pp. 2773–2782, 2000.
[8] J. W. Allran, and W. H. Karasov, "Effects of atrazine on embryos, larvae, and adults of anuran amphibians,” Environ. Toxicol. Chem., vol. 20, pp. 769–775, 2001.
[9] M. L. de Souza, D. Newcombe, S. Alvey, D. E. Crowley, A. Hay, M. J. Sadowsky, and L. P. Wackett, "Molecular Basis of a Bacterial Consortium: Interspecies Catabolism of Atrazine,” Appl. Environ. Microbiol., vol. 64, no. 1, pp. 178–184, Jan. 1998.
[10] J. Sambrook, E. F. Fritsch, and T. Maniatis, "Molecular Cloning: A Laboratory Manual,” Cold Spring Harbor Laboratory, New York, 545 p, 1989.
[11] S. F. Altschul, W. Gish, W. Miller, E. W. Myers, and D. J. Lipman, "Basic local alignment search tool,” J. Mol. Biol., vol. 215, pp. 403–410, 1990.
[12] J. D. Thompson, D. G. Higgins, and T. J. Gibson, "CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice,” Nucleic Acids Res., vol. 22, pp. 4673–4680, 1994.