Serological IgG Testing to Diagnose Alimentary Induced Diseases and Monitoring Efficacy of an Individual Defined Diet in Dogs
Authors: Anne-Margré C. Vink
Background. Food-related allergies and intolerances are frequently occurring in dogs. Diagnosis and monitoring according ‘Golden Standard’ of elimination efficiency is, however, time consuming, expensive, and requires expert clinical setting. In order to facilitate rapid and robust, quantitative testing of intolerance, and determining the individual offending foods, a serological test is implicated for Alimentary Induced Diseases and manifestations. Method. As we developed Medisynx IgG Human Screening Test ELISA before and the dog’ immune system is most similar to humans, we were able to develop Medisynx IgG Dog Screening Test ELISA as well. In this randomized, double-blind, split-sample, retro perspective study 47 dogs suffering from Canine Atopic Dermatitis (CAD) and several secondary induced reactions were included to participate in serological Medisynx IgG Dog Screening Test ELISA (within < 0,02 % SD). Results were expressed as titers relative to the standard OD readings to diagnose alimentary induced diseases and monitoring efficacy of an individual eliminating diet in dogs. Split sample analysis was performed by independently sending 2 times 3 ml serum under two unique codes. Results. The veterinarian monitored these dogs to check dog’ results at least at 3, 7, 21, 49, 70 days and after period of 6 and 12 months on an individual negative diet and a positive challenge (retrospectively) at 6 months. Data of each dog were recorded in a screening form and reported that a complete recovery of all clinical manifestations was observed at or less than 70 days (between 50 and 70 days) in the majority of dogs (44 out of 47 dogs =93.6%). Conclusion. Challenge results showed a significant result of 100% in specificity as well as 100% positive predicted value. On the other hand, sensitivity was 95,7% and negative predictive value was 95,7%. In conclusion, an individual diet based on IgG ELISA in dogs provides a significant improvement of atopic dermatitis and pruritus including all other non-specific defined allergic skin reactions as erythema, itching, biting and gnawing at toes, as well as to several secondary manifestations like chronic diarrhoea, chronic constipation, otitis media, obesity, laziness or inactive behaviour, pain and muscular stiffness causing a movement disorders, excessive lacrimation, hyper behaviour, nervous behaviour and not possible to stay alone at home, anxiety, biting and aggressive behaviour and disobedience behaviour. Furthermore, we conclude that a relatively more severe systemic candidiasis, as shown by relatively higher titer (class 3 and 4 IgG reactions to Candida albicans), influence the duration of recovery from clinical manifestations in affected dogs. These findings are consistent with our preliminary human clinical studies.
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 Hillier A and Griffin CE (2001) The ACVD task force on canine atopic dermatitis: incidence and prevalence. Veterinary immunology and Immunopathology 81: 147-51
 Bethlehem S, Bexley J, Mueller RS. Patch testing and allergen-specific serum IgE and IgG antibodies in the diagnosis of canine adverse food reactions. Vet ImmunolImmunopathol. 2012 Feb 15;145(3-4):582-9.
 Suen RM, Gordon S. The clinical relevance of IgG food testing through ELISA (enzyme-linked immunosorbent assay. US Biotek Lab 2003: 1-8
 Herman PM, Drost LM. Evaluating the Clinical Relevance of Food Sensitivity Tests: A Single-Subject Experiment. Altern Med Rev 2004;9(2):198-207
 Osborne NJ, Koplin JJ, Martin PE, Gurrin LC, Lowe AJ, Matheson MC, et al.: Prevalence of challenge-proven IgE-mediated food allergy using population-based sampling and predetermined challenge criteria in infants. J Allergy ClinImmunol 2011, 127:668-676.
 Mackie RM. Intestinal permeability and topic disease. Lancet 1981; 1:155
 Poulos LM, Waters AM, Correll PK, Loblay RH, Marks GB: Trends in hospitalizations for anaphylaxis, angioedema, and urticaria in Australia, 1993–1994 to 2004–2005. J Allergy ClinImmunol 2007, 120:878-884.
 Prescott SL, Allen KJ: Food Allergy: Riding the second wave of the allergy epidemic. Pediatr Allergy Immunol 2011, 22:155-160.
 Cianferoni A, Spergel JM. Food Allergy: Review, Classification and Diagnosis.
 Kruszewski J, Raczka A, Kłos M, Wiktor-Jedrzejczak W. High serum levels of allergen specific IgG-4 (asIgG-4) for common food allergens in healthy blood donors. Arch ImmunolTherExp (Warsz). 1994;42(4):259-61
 Perelmutter, L. IgG4: Non-IgE mediated atopic disease. Ann Allergy 1984; 52:648
 Stanworth, D.R., Immunochemical aspects of human IgG4. Clin. Rev. Allergy 1983; 1:183-95
 Brighton, W.D., Frequency of occurrence of IgG (S-TS). Clin. Allergy 1980; 10:97-100
 Wintroub, B.U., Soter, N.A., Biology of the mast cell and its role in cutaneous inflammation. Springer Semin. Immunopathol. 1981; 4:55
 Favrot C, Steffan J, Seewald W and Picco F (2010) A prospective study on the clinical features of chronic canine atopic dermatitis and its diagnosis. Veterinary Dermatology 21: 23-31;
 Carlotti DN and Costargent F (1994) Analysis of positive skin tests in 449 dogs with allergic dermatitis. European Journal of Companion Animal Practice 4: 42–59;
 Griffin CE (1993) Canine atopic disease. In: Current Veterinary Dermatology. Eds CE Griffin, KW Kwochka JM McDonald. Mosby Year Book, St Louis pp 90-120;
 Zimmer A, Bexley J, Halliwell RE, Mueller RS. Food allergen-specific serum IgG and IgE before and after elimination diets in allergic dogs. Vet ImmunolImmunopathol. 2011 Dec 15;144(3-4):442-7.
 Linek M, Favrot C. Impact of canine atopic dermatitis on the health-related quality of life of affected dogs and quality of life of their owners. Vet Dermatol. 2010 Oct;21(5):456-62.
 Eaton KK. Sugars in food intolerance and abnormal gut fermentation. J NutrMed 1992; 3: 295-301
 Koh AY, Köhler JR, Coggshall KT, Van Rooijen N, Pier GB. Mucosal damage and neutropenia are required for Candida albicans dissemination. PLoSPathog 2008;4(2):e35.
 Martín A, Sierra MP, González JL, Arévalo MA. Identification of allergens responsible for canine cutaneous adverse food reactions to lamb, beef and cow's milk. Vet Dermatol. 2004 Dec;15(6):349-56.
 Salkie, M.L. Role of Clinical Laboratory in Allergy Testing. Clin. Biochem.1994; 27:343-355.
 Nakagawa, T. The role of IgG subclass antibodies in the clinical response to immunotherapy in allergic disease. Clin Exp. Allergy 1991; 21:289-96. Allergology International. 2009; 58:457-466
 Veterinary Immunology, Ian R. Tizard, Saunders/Elsevier 8t Edition 2009, page 179-14.3