Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 31819
An Effect of Organic Supplements on Stimulating Growth of Dendrobium Protocorms and Seedlings

Authors: Sunthari Tharapan, Chockpisit Thepsithar, Kullanart Obsuwan


This study was aimed to investigate the effect of various organic supplements on growth and development of Dendrobium discolor’s protocorms and seedlings growth of Dendrobium Judy Rutz. Protocorms of Dendrobium discolor with 2.0 cm. in diameter and seedlings of Dendrobium Judy Rutz at the same size (0.5 cm. height) were sub-cultured on Hyponex medium supplemented with cow milk (CM), soy milk (SM), potato extract (PE) and peptone (P) for 2 months. The protocorms were developed to seedlings in all treatments after cultured for 2 months. However, the best results were found on Hyponex medium supplemented with P was the best in which the maximum fresh and dry weight and maximum shoot height were obtained in this treatment statistically different (p ≤ 0.05) to other treatments. Moreover, Hyponex medium supplemented with P also stimulated the maximum mean number of 5.7 shoots per explant which also showed statistically different (p ≤ 0.05) when compared to other treatments. The results of growth of Dendrobium Judy Rutz seedlings indicated the medium supplemented with 100 mL/L PE enhanced the maximum fresh and dry weigh per explants with significantly different (p ≤ 0.05) in fresh weight from other treatments including the control medium without any organic supplementation. However, the dry weight was not significantly different (p ≤ 0.05) from medium supplemented with SM and P. There was multiple shoots induction in all media with or without organic supplementation ranging from 2.6 to 3 shoots per explants. The maximum shoot height was also obtained in the seedlings cultured on medium supplemented with PE while the longest root length was found in medium supplemented with SM.

Keywords: Fresh weight, in vitro propagation, orchid, plant height.

Digital Object Identifier (DOI):

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2464


[1] C. J. Goh and H. Tan, "Clonal propagation from leaf explants in Renantanda orchid hybrid”. Orchid Rev., vol. 90, pp. 295–296, 1982.
[2] Y. Sagawa and J.T. Kunisaki, "Clonal propagation of orchids by tissue culture”, In: A. Fujiwara, Plant Tissue culture, Maruzen, Tokyo. pp. 683–684, 1982.
[3] C. J. Goh, A.A. Sim, and G. Lim, "Mycorrhizal associations in some tropical orchid”, Lindleyana, Vol. 7(1), pp. 13-17, 1992.
[4] F. M. L. Fu, "Studies on the tissue culture of orchids1.Clonal propagation of Phalaenopsis by lateral buds from flower stems”, Orchid Rev., vol. 86, pp. 308–310, 1978
[5] C. C. Lin, "In vitro culture of flower stalk internodes of Phaloenopsis and Doritaenopsis”, Lindlayana, vol. 1, pp.158–163, 1986.
[6] M. Tanaka, "Studies on the clonal propagation of Phalaenopsis through in vitro culture”, Mem Fac. Agric., vol. 49, pp.1–85, 1987.
[7] M. Kobayashi, M. Komatuda, and S. Yonai, "Studies on the vegetative propagation of Phalaenopsis through root tip culture”, Japan Soc. Hort. Sci., vol. 59, pp. 664–665, 1991.
[8] S. Ichihashi, "Micropropagation of Phalaenopsis through the culture of lateral buds from young flower stalks”, Lindleyana, vol.7, pp. 208– 215, 1992.
[9] K. Tokuhara and M. Mii, "Micropropagation of Phalaenopsisby culturing shoot tips of flower stalk buds”, Plant Cell Rep., vol. 13(1), pp. 7–11, 1993.
[10] J. Arditti, "Factors affecting the germination of orchid seeds”. Bot. Rev., vol. 3, pp. 1–97, 1967.
[11] M. Fonnesbech, "Growth hormones and propagation of Cymbidium in vitro”, Physiologia Plantarum, vol. 27, pp. 310–316. 1972.
[12] M. Flamee, "Influence of selected media on the germination and growth of Paphiopedilum seedlings”, Am. Orchid Soc. Bull., vol. 47, pp. 419–423. 1978.
[13] V. H. Mathews and P. S. Rao, "In vitro multiplication of Vanda hybrids through tissue culture technique”, Plant Sci. Lett, vol. 17, pp. 383–389, 1980.
[14] P. T. Krishnamohan and S. M. Jorapur, "In vitro seed culture of Acampe praemorsa (Roxb.) Blatt. and McC”, In: Vij S. P. (ed.), Biology, Conservation and Culture of Orchids, 1986, New Delhi, East- West Press: pp. 437–439.
[15] C. Goh and P. F. Wang, "Micropropagation of the monopodial orchid hybrid Aranda ‘Deborah’ using inflorescence explants”, Sci Hortic- Amsterdam, vol. 44, pp. 315–321, 1990.
[16] L. Villolobus and A. M. Munoz, "Tissue culture of orchids Cattleya, Encyclia, Oncidium and Stanhopea”, Orchid Rev., vol. 102, pp. 58–62, 1994.
[17] R. Sudeep, P. K. Rajeevan, P. K. Valasalakumari, and C. K. Geetha, "Influence of organic supplements on shoot proliferation in Dendrobium”, J. of Hort., vol.3, pp. 38–44, 1997.
[18] S. K. Bhadra and M. M. Hossain, "In vitro germination and micropropagation of Geodorum densiflorum (Lam.) Schltr. an endangered orchid species”, Plant Tiss. Cult. and Biotech., vol. 13, pp. 165–171, 2003.
[19] I. Chowdhury, A. R. M. Rahman, M. O. Islam, and S. Matsui, "Effect of plant growth regulators on callus proliferation, plantlet regeneration and growth of plantlets of Doritaenopsis orchid”, Biotech., vol. 2, 214– 221, 2003.
[20] Y. L. Lee and N. Lee, "Plant regeneration from protocorm derived callus of Cypripedium formosanum”, In Vitro Cell Dev.- Pl., vol. 39, pp. 475–479, 2003.
[21] C. Lekha Rani, C. Vidya, K. Rajmohan, and S. T. Mercy, "Protocorm differentiation and seedling growth in Dendrobium hybrid seed cultures as influenced by organic additives”, The J. of the Orchid Soc. of India, vol. 19, pp. 67–70, 2005.
[22] R. Murdad, S. K. Hwa, C. K. Seng, A. M. Latip, A. Z. Aziz, and R. Ripin, "High frequency multiplication of Phalaenopsis gigantean using trimmed bases protocorms technique”, Sci Hortic-Amsterdam, vol. 111, pp. 73–79, 2006.
[23] S. Aktar, K. M. Nasiruddin, and K. Hossain, "Effects of different media and organic additives interaction on in vitro regeneration of Dendrobium orchid”, J. of Agri. and Rural Develop., vol. 6, pp. 69–74, 2008.
[24] P.I. Hong, J. Chen, and W. Chang, "Shoot development and plant regeneration from protocorm-like bodies of Zygopetalummackayi”,In Vitro Cell. and Develop. Biol. Plant, vol.46, pp. 306–311, 2010.
[25] J. Arditti, "Aspects of physiology of orchids”, Adv. Bot. Res., vol.7, pp. 421–655, 1979.
[26] J. T. Chen and W. C. Chang, "Effects of tissue culture conditions and explants characteristics on direct somatic embryogenesis in Oncidium ‘Gower Ramsey”, Plant Cell Tiss Org., vol. 69, pp. 41–44, 2002.
[27] S. Ichihashi and M. O. Islam, "Effect of complex organic additives on callus growth in three orchid genera, Phalaenopsis, Doritaenopsis and Neofinetia”,J. of JPN Soc. of Hort. Sci., vol. 68, pp. 269–274, 1999.
[28] J. T. Curtis, "Studies on nitrogen nutrition of orchid embryos. I. Complex nitrogen sources”. Am. Orchid Soc. Bull., vol. 16, pp. 654– 660, 1947.
[29] M. Bejoy, S. C. Kumar, B. J. Radhika, and J. Joemon, "Asymbiotic seed germination and early seedling development of the dove orchid Peristeria elata Hook”, The J. of the Orchid Soc. of India, vol. 17, pp. 75–79, 2004.
[30] R. Jost, "Milk and Dairy Products" Ullmann's Encyclopedia of Industrial Chemistry, Wiley-VCH, Weinheim. 2002.
[31] K. Liu, "Soybeans: Chemistry, Technology and Utilization”, Chapman & Hall. 1997.