Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 30309
Comparison of Classical and Ultrasound-Assisted Extractions of Hyphaene thebaica Fruit and Evaluation of Its Extract as Antibacterial Activity in Reducing Severity of Erwinia carotovora

Authors: Hanan Moawad, Naglaa M. Abd EL-Rahman


Erwinia carotovora var. carotovora is the main cause of soft rot in potatoes. Hyphaene thebaica was studied for biocontrol of E. carotovora which inhibited growth of E. carotovora on solid medium, a comparative study of classical and ultrasound-assisted extractions of Hyphaene thebaica fruit. The use of ultrasound decreased significant the total time of treatment and increase the total amount of crude extract. The crude extract was subjected to determine the in vitro, by a bioassay technique revealed that the treatment of paper disks with ultrasound extraction of Hyphaene thebaica reduced the growth of pathogen and produced inhibition zones up to 38mm in diameter. The antioxidant activity of ultrasound-ethanolic extract of Doum fruits (Hyphaene thebaica) was determined. Data obtained showed that the extract contains the secondary metabolites such as Tannins, Saponin, Flavonoids, Phenols, Steroids, Terpenoids, Glycosides and Alkaloids.

Keywords: Ultrasound, Biological Control, classical extract, Erwinia carotovora, Hyphaene thebaica

Digital Object Identifier (DOI):

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2390


[1] J. Wu, L. Lin, F. Chau, Ultrason. Sonochem. 8 (2001) 347.
[2] Z. Hromadkov_a, A. Ebringerov_a, P. Valachovic, Ultrason. Sonochem. 5 (1999) 163.
[3] M. Salisov_a, S. Toma, T.J. Mason, Ultrason. Sonochem. 4 (1997)
[4] S.Q. Huang , Z.X. Ning, Int J Biol Macromol. 47(2010), 336-41.
[5] C. Pappas, P.A. Tarantilis, I. Daliani, T. Mavromoustakos, M. Polissiou, Ultrason. Sonochem. 9 (2002) 19.
[6] L. Paniwnyk, E. Beaufoy, J.P. Lorimer, T.J. Mason, Ultrason. Sonochem. 8 (2001) 299.
[7] M.I.S. Melecchi, M.M. Martinez, F.C. Abad, P.P. Zini, I.N. Filho, E.B. Caramao, Separ. Sci. 25 (2002) 86.
[8] D. Cronin, Y. Moeënne-Loccoz , A. Fenton, C. Dunne, D.N Dowling, and O'Gara, FEMS Microbiology Ecology. 23(1997) 95-106.
[9] A.M. Ahoussi , M. Sébastien, L. Rachid and H. J. Mohamed Biotechnol. Agron. Soc. Environ. 15(1997), 379-386.
[10] V. K. Parthiban, V. Prakasam and K. Prabakar, International Journal of Applied Biology and Pharmaceutical Technology . 3 (2012) 231 - 238 .
[11] Bhat, Masood K. A., Bhat S. D, Ashraf Bhat N. A, Razvi M, Mir S. M., Sabina Akhtar M. R., Wani N. and Habib M. (2010). Current Status of Post Harvest Soft Rot in Vegetables. Asian J.PL.SCI.,(4):200-208
[12] M. V. Jeeshna , S. Paulsamy and T. Mallikadevi, J. Life Sci, 3(2011) 23-27.
[13] A.K. Aremu and O.K. Fadele, African Journal of Agricultural Research 6(2011), 3597-3602
[14] Eldahshan O., Ayoub N., Singab A. and Al-Azizi M., Afr. J.Pharmacy Pharmacol. 3(2009) 158-164.
[15] A.A. El-egami, A.Z. Almagboul, M.E.A. Omar and M.S. El-Tohami, Fitoterapia Part X", 72(2001) 810-817.
[16] J.A Cook, D.J. Vander Jagt, A. Dasgupta, R.S. Glew, W. Blackwell and RH Glew, Life-Science 63(1998) 106-110.
[17] H.O. Edeoga, D.E. Okwu, and B.O. Mbaebie, African J. Biotechnol. 4 (2005) 685-688.
[18] Y.E. Saleh, M.I. Naguib and H.H. Ell-Hemdawy, Egypt. J. Bot. (1984) 75-80.
[19] Y.E. Saleh and M.S. Khalil, Egypt. J. Microbiol. 2 (1991) 171-182.
[20] S.H. Lim, I. Darah and K. Jain, J. Tropical Forest Sci. 18 (2006) 59-65.
[21] G.E. Trease and W.C. Evans, Pharmacogsy. 11th edn. (1989) Brailliar Tiridel Can. Macmillian publishers.
[22] J.B. Harborne. Phytochemical methods, London. Chapman and Hall, Ltd. (1973) 49-188
[23] A. Sofowara, Madicinal plants and Traditional medicine in Africa Spectrum Books Ltd, Ibadan, Nigeria. (1993) P . 289.
[24] B.O. Obdoni, and P.O. Ochuko, Global J. Pure Appl. Sci. 8 (2001) 203-208.
[25] M.S.H. Ahmed, A.S. Zeinab and A.H. Nefisa, Pol. J. Food Nutr. Sci., 60, (2010) 237-242
[26] J.B. Jeong, S.Y. Ju, J.H. Park, J.R. Lee, K.W. Yun, S.T. Kwon, J.H. Lim, G.Y. Chung and H.J. Jeong, Cance Epidem.33 ( 2009) 41–46.
[27] H. Betty, M.I. Coupar and N. Ken, Food Chem. 98 (2006) 317–328.
[28] J.L. Ríos and M.C. Recio, J. Ethnopharmacol 100 ( 2005) 80–84.
[29] M.S.H. Ahmed, A.S. Naglaa, H.A.K Hanan and M.H.A Shams El-Din, Pol. J. Food Nutr. Sci, 61(2011) 201-209.