Inhibitory Effects of Ambrosia trifida L. on the Development of Root Hairs and Protein Patterns of Radicles

Authors: Ji-Hyon Kil, Kew-Cheol Shim, Kyoung-Ae Park, Kyoungho Kim

Abstract:

Ambrosia trifida L. is designated as invasive alien species by the Act on the Conservation and Use of Biodiversity by the Ministry of Environment, Korea. The purpose of present paper was to investigate the inhibitory effects of aqueous extracts of A.trifida on the development of root hairs of Triticum aestivum L., and Allium tuberosum Rottler ex Spreng and the electrophoretic protein patterns of their radicles. The development of root hairs was inhibited by increasing of aqueous extract concentrations. Through SDS-PAGE, the electrophoretic protein bands of extracted proteins from their radicles were appeared in controls, but protein bands of specific molecular weight disappeared or weakened in treatments. In conclusion, inhibitory effects of A. trifida made two receptor species changed morphologically, and at the molecular level in early growth stage.

Keywords: Ambrosia trifida L., invasive alien species, inhibitory effect, root hair, electrophoretic protein, radicle.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1093410

References:

[1] C. Kong, P. Wang and X. Xu, "Allelopathic Interference of Ambrosia trifida with Wheat (Triticum aestivum)”, Agriculture, Ecosystem & Environment, vol. 119, no. 3-4, Mar. 2007, pp. 416-420.
[2] T. R. Seastedt, R. M. Callaway, J. L. Pollock and J. Kaur, "Allelopathy and plant invasions: Traditional, congeneric, and bio-geographical approaches,” Biological Invasions, vol. 10, no. 6, Aug. 2008, pp. 875-890.
[3] J. H. Kil, D. H. Lee, S. M. Hwang, C. W. Lee, Y. H. Kim, D. E. Kim, H. M. Kim, and J. C. Lee, Detailed studies on invasive alien species and their management (VII). The Report of National Institute of Environmental Research, NIER-RP2012-128, 2012.
[4] R. M. Callaway, and E. T. Aschehoug, "Invasive plants versus their new and old neighbors: A mechanism for exotic invasion”, Science, vol. 290, no. 5491, Oct. 2000, pp. 521–523.
[5] K. S. Koh, M. H. Suh, J. H. Kil, Y. B. Ku, H. K. Oh, D. G. Rhee, S. H. Park, E. S. Cheon, and Y. H. Yang, Research on the effects of alien plants on ecosystem and their management (III). The Report of National Institute of Environmental Research (South Korea), 2002.
[6] R. M. Callaway, W. M. Ridenour, T. Laboski, T. Weir, and J. M. Vivanco, "Natural selection for resistance to the allelopathic effects of invasive plants”, Journal of Ecology, vol. 93, no. 3, Apr. 2005, pp. 576-583.
[7] H. J. Cameron, and G. R. Julian, "Inhibition of protein synthesis in lettuce (Latuca sativa L.) by allelopathic compounds”, Journal of Chemical Ecology, vol. 6, no. 6, Jun. 1980, pp. 989-995.
[8] K. A. Park, K. C. Shim, J. H. Kil, and S. H. Yeau, "Allelopathic effects of aqueous extracts from Eupatorium rugosum Houtt. and Erigeron annus L. on radicles growth of Lactuca sativa and Raphanus raphanistroides”, Allelopathy Journal, vol. 27, no. 1, Jan. 2011, pp. 65-74.
[9] J. H., Kil, and K. C. Shim, "Allelopathic effects of Tagetes minuta L. and Eupatorium rugosum Houtt. aqueous extracts on seedling growth of some plants”, Allelopathy Journal, vol. 18, no. 2, Oct. 2006, pp. 315-322.
[10] Z. Y. Ashrafi, S. Sadeghi, H. R. Mashhadi, and M. A. Hassan, "Allelopathic effects of sunflower (Helianthus annuus) on germination and growth of wild barley (Hordeum spontaneum)”, International Journal of Agricultural Technology, vol. 4, no. 1, Jun. 2008, pp. 219-229.
[11] E. Levizou, P. Karageorgou, G. K.Psaras, and Y. Manetas, "Inhibitory effects of water soluble leaf leachates from Dittrichia viscosa on lettuce root growth, statocyte development and graviperception”, Flora, vol. 197, no. 2, Apr. 2002, pp.152-157.
[12] H. S. Namkeleja, M. T. Tarimo, and P. A. Ndakidemi, "Allelopathic effect of aqueous extract of Argemone mexicanaL on germination and growth of Brachiaria dictyoneura L and Clitoria ternatea L", American Journal of Plant Sciences, vol. 4, no. 11, Nov. 2013, pp. 2138-2147.
[13] J. H..Kil, K. C. Shim, K. J. Lee, "Allelopathy of Tagete minuta L. aqueous extracts on seed germination and root hair growth”, Korean J. Ecology, vol. 25, no. 6, Dec. 2002, pp. 395-398.
[14] Ministry of Environment (Korea), Environment Notice No. 2013-12, 02 Feb. 2013.
[15] S. Y. Lee, K. C. Shim, and J. H. Kil, "Phytotoxic effect of aqueous extracts and essential oils from southern marigold (Tagetes minuta)”, New Zealand J. of Crop and Hort. Sci., vol. 30, no. 3, Sep. 2002, pp.161-169.
[16] U. K. Laemmli, "Cleavage of structural proteins during the assembly of the head of bacteriophage T4”, Nature, vol. 227, no. 5259, Aug. 1970, pp. 680-685.
[17] Y. O. Kim, and H. J. Lee, "Effects of aqueous extracts of Pinus rigida on protein and isozyme patterns during radish germination” Korean. J. Ecology, vol. 21, no. 6, Dec. 1998, pp. 771-777.
[18] R. Cruz-Ortega, A. L. Anaya, B. E. Hernandez-Bautista, and G. Laguna-Hernandez, "Effects of allelochemical stress produced by Sicyos deppei on seedling root ultrastructure of Phaseolus vulgaris and Cucurbita ficifolia”, Journal of Chemical Ecology, vol. 24, no. 12, Dec. 1998, pp. 2039-2057.
[19] L. Dolan, "How and where to build a root hair”, Current opinion in Plant Biology, vol. 4, no.6, Dec. 2001, pp. 550-554.
[20] T. Romero-Romero, A. L. Anaya, and R. Cruz-Ortega, "Screening for effects of phytochemical variability on cytoplasmic protein synthesis pattern of crop plants”, Journal of Chemical Ecology, vol. 28, no. 3, Mar. 2002, pp. 617-629.
[21] E. L. Rice, Allelopathy. London, UK: Academic Press, 1984.
[22] S. Gilroy and D. L. Jones, "Through form to function: root hair development and nutrient uptake”, Trends in Plant Science, vol. 5, no. 2, Feb. 2000, pp. 56-60.