The Effects of Seasonal Variation on the Microbial-N Flow to the Small Intestine and Prediction of Feed Intake in Grazing Karayaka Sheep

Authors: Mustafa Salman, Nurcan Cetinkaya, Zehra Selcuk, Bugra Genc

Abstract:

The objectives of the present study were to estimate the microbial-N flow to the small intestine and to predict the digestible organic matter intake (DOMI) in grazing Karayaka sheep based on urinary excretion of purine derivatives (xanthine, hypoxanthine, uric acid, and allantoin) by the use of spot urine sampling under field conditions. In the trial, 10 Karayaka sheep from 2 to 3 years of age were used. The animals were grazed in a pasture for ten months and fed with concentrate and vetch plus oat hay for the other two months (January and February) indoors. Highly significant linear and cubic relationships (P<0.001) were found among months for purine derivatives index, purine derivatives excretion, purine derivatives absorption, microbial-N and DOMI. Through urine sampling and the determination of levels of excreted urinary PD and Purine Derivatives / Creatinine ratio (PDC index), microbial-N values were estimated and they indicated that the protein nutrition of the sheep was insufficient.

In conclusion, the prediction of protein nutrition of sheep under the field conditions may be possible with the use of spot urine sampling, urinary excreted PD and PDC index. The mean purine derivative levels in spot urine samples from sheep were highest in June, July and October. Protein nutrition of pastured sheep may be affected by weather changes, including rainfall. Spot urine sampling may useful in modeling the feed consumption of pasturing sheep. However, further studies are required under different field conditions with different breeds of sheep to develop spot urine sampling as a model.

Keywords: Karayaka sheep, spot sampling, urinary purine derivatives, PDC index, microbial-N, feed intake.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1092409

References:

[1] Turkish Statistical Institute (TurkStat): Livestock Statistics, Url:http://www.turkstat.gov.tr, 2008.
[2] Chen XB, Mejia AT, Kyle DJ, Ørskov ER: Evaluation of the use of the purine derivative: creatinine ratio in spot urine and plasma samples as an index of microbial protein supply in ruminants: studies in sheep. J AgrSci, 125:137-143,1995.
[3] Cetinkaya N, Salman M, Genc B: Estimation of the microbial N flow to small intestine in Saanen goats and kids based on urinary excretion of purin derivatives by the use of spot urine sampling technique. KafkasUniv Vet Fak Derg,16(1):75-79, 2010.
[4] Fujihara T, Orskov ER, Reeds PJ, Kyle DJ: The effect of protein infusion on urinary excretion of pürine derivatives in ruminants nourished by intragastric infusion. J AgricSciCamb, 109:7-12, 1987.
[5] IAEA: A training package the technique for estimating microbial protein supply in ruminants based on determination on pürine derivatives in urine. Joint FAO/IAEA Division of Nuclear Applications in Food and Agriculture. Vienna, Austria, 2003.
[6] Wanapat M, Pimpa, O: Effect of ruminal NH3-N levels on ruminal fermentation, purine derivatives, digestibility and rice straw intake in swamp buffaloes. J AnimSci, 12: 904, 1999.
[7] Puchala R, Kulasek GW: Estimation of microbial protein flow from the rumen of sheep using microbial protein flow from the rumen of sheep using microbial nucleic acid and urinary excretion of pürine derivatives. Can J AnimSci, 72, 821-830, 1992.
[8] Makkar HPS, Chen XB: Estimation of microbial protein supply in ruminants using urinary purine derivatives, Springer 1 ed. VII, p.212, 2004.
[9] Antoniewicz AN, Hienemann WW, Hanks EM: The effect of changes in the flow of nucleic acids on allantoin excretion in the urine of sheep. J AgricSci, 95: 395-400, 1980.
[10] Chen XB, Chen YK, Franklin MF, Ørskov ER, Shand, WJ: The effect of feed intake and body weight on purine derivative excretion and microbial protein upply. J AnimSci, 70:1534–1542, 1992.
[11] Chen XB, Gomes JM: Estimation of microbial protein supply to sheep and cattle based on urinary excretion of purine derivatives and overview of the technical detail. International feed resources unit. Rowett Research Institute, Bucksburn Aberdeen, UK. Occasional publication, pp.1-21, 1992.
[12] Karabulut A, Canbolat Ö: Feed evaluation and analyze methods. Uludag University Publ., no: 2,05.048,0424, Bursa, 2005.
[13] AOAC: Official Methods of Analysis, 15th ed. Association of Official Agricultural Chemists, Washington, DC, 1995.
[14] Van Soest PJ, Robertson JB, Lewis, BA: Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci, 74:3583, 1991.
[15] Chen XB, Hovell FD, De B, Orskov ER, Brown DA: Excretion of purine derivatives by ruminants: effect of exogenous nucleic acid supply on purine derivative excretion by sheep. Br J Nutr, 63:131-142,1990.
[16] Agricultural Research Council (ARC): The nutrient requirements of ruminant livestock. Suppl. No.1, Commonwealth Agricultural Bureaux, Slough, 1984.
[17] SAS Statistical Software: SAS Compus drive, Carry, NC 27513 USA, 2009.
[18] McAllan AB, Smith RH: Degradation of nucleic acids in the rumen. Br J Nutr, 29:331-345, 1973.
[19] Brun-Bellut J, Linberg JE, Hadjipanayiotou M: Protein Nutrition and Requirements of Adult Dairy Goats. In, Morand-Fehr P (Ed): Goat Nutrition. EAAP Publ. No.46, PudocWageningen Netherlands, pp. 82-93, 1991.
[20] Linberg JE: Urinary allantoin excretion and digestible organic matter intake in dairy goats. Swed J Agric Res, 15, 3137, 1985.
[21] Ma T, Deng K, Jiang C, Tu Y, Zhang N, Liu J, Zhao Y, Diaoa, Q: The relationship between microbial N synthesis and urinary excretion of purine derivatives in Dorper×thin-tailed Han crossbred sheep, http://dx.doi.org/10.1016/j.smallrumres.2012.09.003, 2012.
[22] Cetinkaya N, Yaman S, Baber NHO: The use of purine derivatives/creatinine ratio in spot urine samples as an index of microbial protein supply in Yerli Kara crossbred cattle. LivestSci, 100:91-98, 2006.
[23] Gonzalez-Ronquillo M, Balcells J, Belenguer A, Castrillo C, Mota M: A comparison of purine derivatives excretion with conventional methods as indices of microbial yield in dairy cows. American Dairy Science Association. J Dairy Sci, 87:2211-2221, 2004.
[24] Balcells J, Guada JA, Peiro JM: Simultaneous determination of allantoin and oxypurines in biological fluids by high performance liquid chromatography. J Chromatogr, 575:153-157, 1992.
[25] Orellana-Boeroa P, Seradjb AR, Fondevilac MJ, Noland J, Balcells J: Modelling urinary purine derivatives excretion as a tool to estimate microbial rumen outflow in alpacas (Vicugnapacos), Small Rumin. Res., 107, 101-104, 2012.
[26] Belenguer A, Yanez D, Balcells J, Ozdemir BNH, Gonzalez-Ronquillo M: Urinary excretion of purine derivatives and prediction of rumen microbial outflow in goats. Livest Prod Sci, 77:127-135, 2002.
[27] Carro MD, Cantalapiedra-Hijar G, Ranilla MJ, Molina-Alcaide E: Urinary excretion of purine derivatives, microbial protein synthesis, N utilization, and ruminal fermentation in sheep and goats fed diets of different quality. J AnimSci, 2011-4577, 2012.
[28] Hindrichsen IK, Osuji PO, Odenyo AA, Madsen J, Hvelplund T: Effects of supplementation of a basal diet of maizestover with different amounts of Leucaenadiversifolia on intake, digestibility, nitrogen metabolism and rumen parameters in sheep. Anim Feed SciTechnol, 98: 131-142, 2002.
[29] Kreuzer M, Kirchgessner M, Kellner RJ, Roth FX: Effect of varying protein and energy concentration on digestibility of nutrients, nitrogen metabolism and allantoin excretion in wethers. German J AnimPhysiolAnimNutr 55:144-159, 1986.
[30] Laurent F, Vignon B: Factors of variations in urinary excretion of allantoin in sheep and goats. AnimSci, 64:281-282, 1983.
[31] Jetana T, Suthikrai W, Usawang S, Kijsamrej S, Sophon S: The use urinary purines excreted in the urine for prediction microbial protein production form rumen: Using spot sampling for the prediction microbial protein from the rumen of Brahman Cattle. The 4th Chulalongkorn University Veterinary Annual Conference 60th Veterinary Anniversary Building, Chulalongkorn University, 2005.
[32] Yu P, Boon-ek L, Leury BJ: Effect of dietary protein variation in term of net truly digest intestinal protein (DVE) and rumen degraded protein balance (OEB) on the concentration and excretion of urinary creatinine, purine derivatives and microbial N supply in sheep: comparation with the prediction from the DVE/OEB model. Anim Feed Sci Tech, 93:71-91,2001.