Influence of Fermentation Conditions on Humic Acids Production by Trichoderma viride Using an Oil Palm Empty Fruit Bunch as the Substrate

Authors: F. L. Motta, M. H. A. Santana

Abstract:

Humic acids (HA) were produced by a Trichoderma viride strain under submerged fermentation in a medium based on the oil palm empty fruit bunch (EFB) and the main variables of the process were optimized by using response surface methodology. A temperature of 40°C and concentrations of 50g/L EFB, 5.7g/L potato peptone and 0.11g/L (NH4)2SO4 were the optimum levels of the variables that maximize the HA production, within the physicochemical and biological limits of the process. The optimized conditions led to an experimental HA concentration of 428.4±17.5 mg/L, which validated the prediction from the statistical model of 412.0mg/L. This optimization increased about 7–fold the HA production previously reported in the literature. Additionally, the time profiles of HA production and fungal growth confirmed our previous findings that HA production preferably occurs during fungal sporulation. The present study demonstrated that T. viride successfully produced HA via the submerged fermentation of EFB and the process parameters were successfully optimized using a statistics-based response surface model. To the best of our knowledge, the present work is the first report on the optimization of HA production from EFB by a biotechnological process, whose feasibility was only pointed out in previous works.

Keywords: Empty fruit bunch, humic acids, submerged fermentation, Trichoderma viride.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1092235

References:

[1] G. R. Aiken, D. M. Mcknight,R. L. Wershaw and P. Mac- Carthy,"Humic substances in soil, sediment, and water: geochemistry, isolation and characterization”. Wiley, 1985, 692 p.
[2] Y. P. Chin, S. J Traina,C. R. Swank and D. Backhus, "Abundance and properties of dissolved organic matter in pore waters of a freshwater wetland”. Limnology and Oceanography, vol. 43, no. 6, p. 1287-1296, 1998.
[3] E. Cameron, N. How, S.Saggar and C. Ross, "The cost-benefits of applying biosolid composts for vegetables, fruit and maize/sweetcorn production systems in New Zealand”. Manaaki Whenua Press. 33 p., 2004.
[4] E. Yamada, T. Ozaki and M. Kimura,"Determination and behavior of humic substances as precursors of trihalomethane in environmental water”. Analytical Sciences, vol. 14, no. 2, p. 327-332, 1998.
[5] J. Asing, N. C. Wong and S. Lau, "Optimization of extraction method and characterization of humic acid derived from coals and composts”. Journal of Tropical Agriculture and Food Science, vol. 37, no. 2, p. 211– 223, 2011.
[6] H. Joosten,M. Tapio-Bistrom and S. Tol,"Peatlands – guidance for climate change mitigation through conservation, rehabilitation and sustainable use”. 114p., 2012.
[7] F. Hamzah,A. Idris and T. K.Shuan,“Preliminary study on enzymatic hydrolysis of treated oil palm (Elaeis) empty fruit bunches fibre (EFB) by using combination of cellulase and ȕ 1-4 glucosidase”. Biomass and Bioenergy, vol. 35, no. 3, p. 1055-1059, 2011.
[8] N. A. F. Harun,A. S. Baharuddin,M. H. M. Zainudin,E. K. Bahrin M. N. Naimand R. Zakaria,“Cellulase production from treated oil palm empty fruit bunch degradation by locally isolated Thermobifidafusca”. BioResources, vol. 8, no. 1, p. 676-68, 2013.
[9] S. Vermeulen and G. Nathalie,“Towards better practice in smallholder palm oil production”. Oldacres. 57 p., 2006.
[10] P. O. Oviasogie, N. O. Aisueni and G. E. Brown,“Oil palm composted biomass: A review of the preparation, utilization, handling and storage”. African Journal of Agricultural Research, vol. 5, no. 13, p. 1553-1571, 2010.
[11] I.S. Druzhinina,V. Seidl-Seiboth,A. Herrera-Estrella,B. A. Horwitz, C. M. Kenerley, E. Monte, P. K. Mukherjee,S. Zenlinger,I. V. Grigoriev and C. P. Kubicek,“Trichoderma: the genomics of opportunistic success”. Nature Reviews Microbiology, vol. 9, no. 10, p. 749-759, 2011.
[12] H. I. Al-Taweil, M. B. Osman, A. H. Aidil, and W. M. W. Yussof, “Optimizing of Trichodermaviride cultivation in submerged state fermentation”. American Journal of Applied Sciences, vol. 6, no. 7, p. 1284-1288, 2009.
[13] M. Verma,S. K. Brar, R. D. Tyagi, R. Y. Surampalliand J. R. Valero, “Dissolved oxygen as principal parameter for conidia production of biocontrol fungi Trichodermaviride in non-Newtonian wastewater”. Journal of Industrial Microbiology and Biotechnology, vol. 33, no. 11, p. 941-952, 2006
[14] P. Tauro, K. K. Kapoor, K. S. Yadav,“An introduction to microbiology”. New Age International. 412 p. 2004.
[15] M. A. Jackson,“Optimizing nutritional conditions for the liquid culture production of effective fungal biological control agents”. Journal of Industrial Microbiology and Biotechnology, vol. 19, no. 3, p. 180-187, 1997.
[16] C. R. Soccol, A. Pandey and C, Larroche,“Fermentation Processes Engineering in the Food Industry”. CRC Press. 510 p, 2013.
[17] F. L. Motta and M. H. A. Santana,“Production of humic acids from oil palm empty fruit bunch by submerged fermentation with Trichodermaviride: cellulosic substrates and nitrogen sources”. Biotechnology Progress, vol. 29, no. 3, p. 631-637, 2013.
[18] R. L. Plackett and J. P Burman, “The design of optimum multifactorial experiments”. Biometrika, vol. 33, no. 4, p. 305-325, 1946
[19] F. L. Motta and M. H. A. Santana,“Biomass production from Trichodermaviride in non-conventional oat medium”. Biotechnology Progress, vol. 28, no. 5, p. 1245-1250, 2012.
[20] A. Badis, F. Z. Ferradji, A. Boucherit, D. Fodil and H. Boutoumi, “Removal of natural humic acids by decolorizing actinomycetes isolated from different soils (Algeria) for application in water purification”. Desalination, vol. 259, no. 1-3, p. 216-222, 2010.
[21] N. Callow and L. Ju,“Promoting pellet growth of Trichodermareesei Rut C30 by surfactants for easy separation and enhanced cellulase production”. Enzyme and Microbial Technology, vol. 50, no. 6-7, p. 311-317, 2012.
[22] P. K. Smith,R. I. Krohn, G. T.Hermanson, A. K. Mallia, F. H. Gartner, M. D. Frovenzano, E. K. Fujimoto, N. M. Goeke, B. J. Olson and D. C. Klenk, “Measurement of protein using bicinchoninic acid”. Analytical Biochemistry, vol. 150, no. 1, p. 76-85, 1985.
[23] S. L. Bhattiprolu, “Growth of Trichodermaviride as influenced by ph, temperature, botanicals, fungicides and mutagenic agent”. Indian Journal of Plant Protection, vol. 36: no. 2, p. 279-282, 2008.
[24] M. Schintzer and S. U. Khan,“Soil Organic Matter”. Elsevier. 319 p., 1978.
[25] S. Anastassiadisand H. J. Rehm, “Oxygen and temperature effect on continuous citric acid secretion in Candida oleophila”. Electronic Journal of Biotechnology, vol. 9, no. 4, p. 341-350, 2006.
[26] A. D., Juwonans O. F. Emmanuel, “Experimental investigations on the effects of carbon and nitrogen sources on concomitant amylase and polygalacturonase production by Trichodermaviride BITRS-1001 in submerged fermentation”. Biotechnology Research International, 2012.
[27] R. R. Singhania, R. K. Sukumaran and A. Pandey,“Improved cellulase production by Trichodermareesei RUT C30 under SSF through process optimization. Applied Biochemistry and Biotechnology, vol. 142, no. 1, p. 60-70, 2007.
[28] A. S. A. El-Sayed, “L-glutaminase production by Trichodermakoningii under solid state fermentation”. Indian Journal of Microbiology, vol. 49, no. 3, p. 243-250, 2009.
[29] M. Z. Alam, S. A. Muyibi and R. Wahid, “Statistical optimization of process conditions for cellulase production by liquid state bioconversion of domestic wastewater sludge”. Bioresource Technology, vol. 99, no. 11, p. 4709-4716, 2008.
[30] C. Christias, C. Couvaraki, S. G.Georgopoulos,B. Macris and V. Vomvoyanni,“Protein Content and Amino Acid Composition of Certain Fungi Evaluated for Microbial Protein Production”. Journal of Applied Microbiology, vol. 29, no. 2, p. 250-254, 1975.
[31] Y. Siddiqui,S. Meon,R. Ismail, M. Rahmani and A. Ali, “In vitro fungicidal activity of humic acid fraction from oil palm compost”. International Journal of Agriculture and Biology, vol. 11, no. 4, p. 448- 452, 2009.