Plants and Microorganisms for Phytoremediation of Soils Polluted with Organochlorine Pesticides
The goal of presented work is the development phytoremediation method targeted to cleaning environment polluted with organochlorine pesticides, based on joint application of plants and microorganisms. For this aim the selection of plants and microorganisms with corresponding capabilities towards three organochlorine pesticides (Lindane, DDT and PCP) has been carried out.
The tolerance of plants to tested pesticides and induction degree of plant detoxification enzymes by these compounds have been used as main criteria for estimating the applicability of plants in proposed technology. Obtained results show that alfalfa, maize and soybean among tested six plant species have highest tolerance to pesticides.
As a result of screening, more than 30 strains from genera Pseudomonas have been selected. As a result of GC analysis of incubation area, 11 active cultures for investigated pesticides are carefully chosen.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1092118Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2460
 Tsao, D.T. "Phytoremediation. Advances in Biochemical Engineering and Biotechnology”. Springer, Berlin Heidelberg New York, 2003.
 Kvesitadze, G., Khatisashvili, G., Sadunishvili, T., Ramsden, J.J. "Biochemical Mechanisms of Detoxification: Basis of Phytoremediation”. Springer,Berlin Heidelberg New York, 2006.
 Kurasvili M.V., Adamia G.S, Ananiasvili T.I., Varazi T.G., Pruidze M.V., Gordeziani M.S.,Khatisashvili G.A. "Plants as tools for control and remediation of environment polluted by organochlorine toxicants”. Ann.Agrar. Sci., submitted for publication.
 Gregory, R., Bendall, D. "The purification and properties of the polyphenol oxidase from tea”. J. Biochem., 101, pp. 569-581, 1966.
 Lanzarini, G., Pifferi, P., Samorani, A. "Specifity of an o-diphenol oxidase from Prunusavium fruits”. Phytochemistry, 11, pp. 89-94, 1972.
 Schroder, P., Rennenberg, H. "Characterization of glutathione S-transferase from dwarf pine needles (PinusmugoTurra)”. Tree Physiol., 11, pp. 151-160, 1992.
 Khatisashvili, G., Kurashvili, M., Gordeziani, M. "Isolation of plant microsomal fraction and characterization of its oxidative systems”. Bull. Georgian Ac. Sci., 152, pp. 818–824, 1995.
 Bradford, M.M. "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the trinciple of protein dye binding”. Anal. Biochem., 59, pp. 277-282, 1974.