Rapid Detection System of Airborne Pathogens

Authors: Shigenori Togashi, Kei Takenaka

Abstract:

We developed new processes which can collect and detect rapidly airborne pathogens such as the avian flu virus for the pandemic prevention. The fluorescence antibody technique is known as one of high-sensitive detection methods for viruses, but this needs up to a few hours to bind sufficient fluorescence dyes to viruses for detection. In this paper, we developed a mist-labeling can detect substitution viruses in a short time to improve the binding rate of fluorescent dyes and substitution viruses by the micro reaction process. Moreover, we developed the rapid detection system with the above “mist labeling”. The detection system set with a sampling bag collecting patient’s breath and a cartridge can detect automatically pathogens within 10 minutes.

Keywords: Viruses, Sampler, Mist, Detection, Fluorescent dyes, Microreaction.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1091476

References:

[1] W. J. McKibbin and A. A. Sidorenko., The Global Costs of an Influenza Pandemic, The Milken Institute Review Third Quarter 2007, pp. 18-27, (2007).
[2] T. P. Weber and N.I. Stilianakis., Inactivation of Influenza A Viruses in the Environment and Modes of Transmission: A Critical Review, J Infect, Vol. 57, No. 5, pp. 361-373, (1991).
[3] K. Takenaka, et al., Airborne Virus Micro-hole Sampler designed by Particle Track Analysis for the Pandemic Prevention, NMC2011 Digest, 25C-2-5 (2011).
[4] A. J. Ozinskas., Principles of Fluorescence Immunoassay, Topics in Fluorescence Spectroscopy, 4, pp. 449-496 (2002).
[5] P. Monaghan, et al., Use of Confocal Immunofluorescence Microscopy To Localize Viral Nonstructural Proteins and Potential Sites of Replication in Pigs Experimentally Infected with Foot-and-Mouth Disease Virus, J. Virol, 79, pp. 6410-6418 (2005).
[6] N. Honda., Studies on High Performance Micro Immunoassay Systems, PhD Thesis, Waseda University.