In vitro and in vivo Anticholinesterase Activity of the Volatile Oil of the Aerial Parts of Ocimum basilicum L. and O. africanum Lour. Growing in Egypt
In this study, the in vitro anticholinesterase activity of
the volatile oils of both O. basilicum and O. africanum was
investigated and both samples showed significant activity. The major
constituents of the two oils were isolated using several column
chromatographies. Linalool, 1,8-cineol and eugenol were isolated
from the volatile oil of O. basilicum and camphor was isolated from
the volatile oil of O. africanum. The anticholinesterase activities of
the isolated compounds were also evaluated where 1,8-cineol showed
the highest inhibitory activity followed by camphor. To confirm these
activities, learning and memory enhancing effects were tested in
mice. Memory impairment was induced by scopolamine, a
cholinergic muscarinic receptor antagonist. Anti-amnesic effects of
both volatile oils and their terpenoids were investigated by the
passive avoidance task in mice. We also examined their effects on
brain acetylcholinesterase activity. Results showed that scopolamineinduced
cognitive dysfunction was significantly attenuated by
administration of the volatile oils and their terpenoids, eugenol and
camphor, in the passive avoidance task and inhibited brain
acetylcholinesterase activity. These results suggest that O. basilicum
and O. africanum volatile oils can be good candidates for further
studies on Alzheimer’s disease via their acetylcholinesterase
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1337073Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 3101
 K. L. Alikatte, B. R. Aakondi, V. G. Yerragunta, P.R. Veerareddy, S. Palle, "AntiamnesicActivity of Syzygiumcumini against Scopolamine Induced Spatial Memory Impairments in Rats,”Brain Dev, vol. 34, pp. 844-851, 2012.
 R.N. Kalaria, G.E. Maestre, R. Arizaga, R.P. Friedland, D. Galasko, K. Hall, J.A. Luchsinger, A. Ogunniyi, E.K. Perry, F. Potocnik, M. Prince, R. Stewart, A. Wimo, Z. Zhang, and P. Antuono, "Alzheimer’s Disease and Vascular Dementia in Developing Countries: Prevalence, Management, and Risk Factors,” The Lancet Neurology, vol. 7, pp. 812–826, 2008.
 J.L. Cummings, H.V. Vinters, G.M. Cole, and Z.S. Khachaturian, "Alzheimer’s Disease: Etiologies, Pathophysiology, Cognitive Reserve and Treatment Opportunities,” Neurology, vol. 51, S2–S17, 1988.
 P.T. Francis, A.M. Palmer, M. Snape, and G.K. Wilcock, "The Cholinergic Hypothesis of Alzheimer’s Disease: A Review of Progress,” Journal of Neurology Neurosurgery and Psychiatry, vol. 66, pp. 137–147, 1999.
 D.K. Lahiri, M.R. Farlow, N.H. Greig, and K. Sambamurti, "Current Drug Targets for Alzheimer’s Disease Treatment,” Drug Development Research, vol. 56, pp. 267-281, 2002.
 S. Thompson, K.L. Lanctot, and N. Herrmann, "The Benefits and Risks Associated with Cholinesterase Inhibitor Therapy in Alzheimer’s Disease,” Expert Opinion on Drug Safety, vol.3, pp. 425–440, 2004.
 L. Fang, B. Kraus, J. Lehmann, J. Heilmann, Y. Zhang, and M. Decker, "Design and Synthesis of Tacrine–Ferulic Acid Hybrids as Multi-Potent anti-Alzheimer Drug Candidates,”Bioorganic and Medicinal Chemistry Letters, vol. 18, pp. 2905–2909, 2008.
 V. Singh, S. Amdekar, and O. Verma, "Ocimum sanctum (Tulsi): Bio-Pharmacological Activities,” WebmedCentral Pharmacology, vol. 1, WMC001046, 2010.
 A. Paton, "A Synopsis ofOcimum L. (Labiatae) in Africa,” Kew Bull, vol. 47, pp. 405, 1992.
 N-A. Zeggwagh, and M. Eddouks, "Anti-Hyperglycaemicand Hypolipidemic Effects of OcimumbasilicumAqueous Extract in Diabetic Rats,” American Journal of Pharmacology and Toxicology, vol. 2, issue 3, pp. 123-129, 2007.
 M.S. Akhtar, and M. Munir, "Evaluation of the Gastric Antiulcerugenic Effects of Solanumnigrum, Brassica oleracea and Ocimumbasilicum in Rats,” J Ethnopharmacol, vol. 27, pp. 163–171, 1989.
 K. Ilhan, Y. Nazife, and B. Mehlika, "Antimicrobial Activity of Various Extracts of Ocimumbasilicum L. and Observation of the Inhibition Effect on Bacterial Cells by Use of Scanning Electron Microscopy,” Afr J Trad CAM , vol. 5, pp. 363–369, 2008.
 T. Dasgupta, A. R.Rao, and P. K. Yadava, "ChemomodulatoryEfficacy of Basil Leaf (Ocimumbasilicum) on Drug Metabolizing and Antioxidant Enzymes, and on Carcinogen-Induced Skin and ForestomachPapillomagenesis,” Phytomedicine, vol. 11, pp. 139–151, 2004.
 O. Stajkovic, B. Tanja, M. Dragana, S. Slavisa, V. G. Branka, S. Draga, and K. V.Jelena, "AntimutagenicProperties of Basil (Ocimumbasilicum L.) inSalmonella typhimurium TA100,” Food TechnolBiotechnol, vol. 45, pp.213–217, 2007.
 E. Capecka, A.Mareczek, and B. M. Leja, "Antioxidant Activity of Fresh and Dry Herbs of Some LamiaceaeSpecies,” Food Chem,vol. 93, pp. 223–226, 2005.
 A. Umar, G. Imam, W. Yimin, and P. Kerim, "Anti Hypertensive Effects of L. on Blood Pressure in Renovascular Hypertensive Rats,” Hyperten Res, vol. 33, pp. 723–730, 2010.
 A. F. Blank, Y.R.Rosa, J. L. CarvalhoFilho, C.A. Santos, M. F. Arrigoni-Blank, E. S. Niculau, et al., "A Diallel Study of Yield Components and Essential Oil Constituents in Basil (Ocimumbasilicum L.), ”Ind Crops Prod, vol. 38, pp. 93-98, 2012.
 R. J. Grayer, G. C. Kite, F. J. Goldstone, S. E. Bryan, A. Paton, and E. Putievsky, "InfraspecificTaxonomy and Essential Oil Chemotypesin Sweet Basil, Ocimumbasilicum,”Phytochemistry, vol. 43, pp.1033-1039, 1996.
 E. Klimánková, K. Holadova, J. Hajslova, T. Cajka, J. Poustka, and M. Koudela, "Aroma Profiles of Five Basil (Ocimumbasilicum L.) Cultivars Grown under Conventional and Organic Conditions,” Food Chem, vol. 107, pp. 464-472, 2008.
 S. Amrani, H. Harnafi, D. Gadi, H. Mekhfi, A. Legssyer, M. Aziz, et al, "Vasorelaxant and Anti-Platelet Aggregation Effects of Aqueous OcimumbasilicumExtract, ”J Ethnopharmacol, vol. 125, pp. 157-162, 2009.
 H. Harnafi, M. Aziz, and S. Amrani, "Sweet Basil (Ocimumbasilicum L.) Improves Lipid Metabolism in Hypercholesterolemic Rats,”E SpenEur E J ClinNutrMetab, vol. 4, pp. 181-186, 2009.
 D.K. Rush, "Scopolamine Amnesia of Passive Avoidance: A Deficit of InformationAcquisition,” Behav. Neural Biol, vol. 50, pp. 255–274, 1988.
 G. L. Ellman, K. D. Courtney, V. Andres, and R. M. Featherstone, "A New and Rapid Colorimetric Determination of Actetylcholinesterase Activity,”Biochem. Pharmacol, vol. 7, pp. 88-95, 1961.
 M.E. Jarvik, and R. Kopp, "An Improved One-Trial Learning Situation in Mice,” Psychol. Rep, vol.21, pp.221–224, 1967.
 D. Dhingra, M. Parle, and S.K. Kulkarni, "Memory Enhancing Activity of Glycyrrhizaglabra in Mice,” J. Ethnopharmacol, vol. 91, pp. 361–365, 2004.
 M. Dokania, K. Kishore, and P. Sharma, "Effect of Ocimum sanctumExtract on Sodium Nitrite-Induced Experimental Amnesia in Mice,” Thai J Pharm Sci, vol. 35, pp. 123-130, 2011.
 S. Kumar, S. Rao, S. Nayak, and N. Sareesh, "Effect of Ocimumsanctum (Linn) Extract on Restraint Stress Induced Behavioral Deficits in Male Wistar Rats, ” PharmacolOnline,vol. 3, pp. 394-404, 2007.
 H. Joshi and M. Parle, "Cholinergic Basis of Memory Improving Effect of OcimumtenuiflorumLinn,” Indian J Pharm Sci, vol. 68, pp. 364, 2006.
 K. S.Bora, S. Arora, and R. Shri,"Role of Ocimumbasilicum L. in Prevention of Ischemia and Reperfusion-Induced Cerebral Damage, and Motor Dysfunctions in Mice Brain,” J Ethnopharmacol, vol. 137, pp. 1360-1365, 2011.
 I. Gülçin, M. Elmasta, and H. Y. Aboul-Enein, "Determination of Antioxidant and Radical Scavenging Activity of Basil (Ocimumbasilicum L. Family Lamiaceae) Assayed by Different Methodologies,” Phytother Res, vol. 21, pp. 354-361, 2007.
 H. J. Chang, H. J. Kim, and H. S. Chun, "Quantitative Structure-Activity Relationship (QSAR) for Neuroprotective Activity of Terpenoids,” Life Sciences, vol. 80, no. 9, pp. 835–841, 2007.
 L. Mu, J. Kou, D. Zhu, and B. Yu, "Comparison of Neuroprotective Effects of Flavonoids, Terpenoids, and Their Combinations from Ginkgo bilobaon Ischemia-Reperfusion—Injured Mice,” Pharmaceutical Biology, vol. 45, no. 9, pp. 728–733, 2007.
 I. Kivrak, M. E. Duru, M. O¨ ztu¨rk, N. Mercan, M. Harmandar, and G. Topc¸u, "Antioxidant, Anticholinesterase and Antimicrobial Constituents from the Essential Oil and Ethanol Extract of Salvia potentillifolia,” Food Chemistry, vol. 116, no. 2, pp. 470-479, 2009.
 K. Shiomi, "Meroterpenoids with Various Biological Activities Produced by Fungi,” Pure and Applied Chemistry, vol. 71, no. 6,pp. 1059–1064, 1999.
 S. U. Savelev, E. J. Okello, and E. K. Perry, "Butyryl- and Acetylcholinesterase Inhibitory Activities in Essential Oils ofSalviaSpecies and Their Constituents,” Phytotherapy Research,vol. 18, no. 4, pp. 315–324, 2004.