CFD Simulation of Hydrodynamic Behaviors and Gas-Liquid Mass Transfer in a Stirred Airlift Bioreactor
Authors: Sérgio S. de Jesus, Edgar Leonardo Martínez, Aulus R.R. Binelli, Aline Santana, Rubens Maciel Filho
Abstract:
The speed profiles, gas holdup (eG) and global oxygen transfer coefficient (kLa) from a stirred airlift bioreactor using water as the fluid model, was investigated by computational fluid dynamics modeling. The parameters predicted by the computer model were validated with the experimental dates. The CFD results were very close to those obtained experimentally. During the simulation it was verified a prevalent impeller effect at low speeds, propelling a large volume of fluid against the walls of the vessel, which without recirculation, results in low values of eG and kLa; however, by increasing air velocity, the impeller effect is smaller with the air flow being greater, in the region of the riser, causing fluid recirculation, which explains the increase in eG and kLa.
Keywords: CFD, Hydrodynamics, Mass transfer, Stirred airlift bioreactor.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1336568
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[1] D.J. Pollard, A.P. Ison, S.P. Ayazi, and M.D. Lilly, "Influence of propeller on Saccharomyces cerevisae fermentation in a pilot scale airlift bioreactor”, Bioprocess Eng. vol. 16, no. 5, pp. 273–281. Apr. 1997.
[2] W. Bang, I. Nikov, H. Delmas and A. Bascoul, "Gas-liquid mass transfer in a new three-phase stirred airlift reactor”, J. Chem. Technol. Biotechnol., vol. 72, pp. 137-142, June 1998.
[3] Y. Chisti and U.J. Jauregui-Haza, "Oxygen transfer and mixing in mechanically agitated airlift bioreactors”, Biochem Eng J., vol. 10, pp. 143-153, Mar. 2002.
[4] D. Ankamma Rao and P. Sivashanmugam, "Experimental and CFD simulation studies on power consumption in mixing using energy saving turbine agitator”, J. Ind. Eng. Chem., vol. 16, pp. 157-161, Jan. 2010.
[5] R. Liu, W. Sun, and C.-Z. Liu, "Computational fluid dynamics modeling of mass transfer behavior in a bioreactor for hairy root culture. I. Model development and experimental validation”, Biotech. Progress, vol. 27, no. 6, pp. 1661-1671, Nov.-Dec. 2011.
[6] H.V. Hristov, R. Mann, V. Lossev and S.D. Vlaev, "A simplified CFD for three-dimensional analysis of fluid mixing, mass transfer and bioreaction in a fermenter equipped with triple novel geometry impellers”, Food Bioprod. Proc., vol. 82, pp. 21–34, Mar. 2004.
[7] Y. Chisti, Airlift bioreactors. Elsevier, New York, 1989, 345 p.
[8] B. Bandyopadhyay, A.E. Humphrey, and H. Taguchi, "Dynamic measurement of the volumetric oxygen transfer coefficient in fermentation systems”, Biotechnol. Bioeng. vol. 9, no. 4, pp. 533–544, Oct. 1967.
[9] J-Y. Xia, Y-H. Wang, S.L. Zhang, N.Chen, P. Yin, Y-P. Zhuang and P. Chu, "Fluid dynamics investigation of variant impeller combinations by simulation and fermentation experiment”, Biochem. Eng. J., vol. 24, pp. 252-260, Oct. 2009.
[10] V.V. Ranade, J.R. Bourne and J.B Joshi, "Fluid mechanics and blending in agitated tanks”, Chem. Eng. Sci., vol. 46, no. 8, pp. 1883–1893, 1991.
[11] Q. Huang, C. Yang, G. Yu and Z-S. Mao, "CFD simulation of hydrodynamics and mass transfer in an internal airlift loop reactor using a steady two-fluid model”, Chem. Eng. Sc., vol. 65, pp. 5527-5536, July 2010.
[12] K. Akita and F. Yoshida, "Bubble size, interfacial area, and liquid-phase mass transfer coefficient in bubble columns”, Ind. Eng. Chem. Proc.Des. Dev., vol. 67, pp. 84-91, June 1974.
[13] S.U. Ahmed, P. R.anganathan, A. Pandey and S. Sivaraman, "Computational fluid dynamics modeling of gas dispersion in multi impeller bioreactor”, J. Biosci. Bioeng., vol. 109, no. 6, pp. 588-597, June 2010.
[14] Y. Chisti and M. Moo-Young, "Gas hold up in pneumatic reactors”, Chem. Eng. J., 38, no. 3, pp. 149-152, July 1988.
[15] D.J. Pollard, A.P. Ison, P.A. Shamlou and M.D. Lilly, "Reactor heterogeneity with Saccharopolyspora erythraea airlift fermentations”, Biotechnol. Bioeng. vol.58, no.5, pp. 453–463, June 1998.
[16] J.B. Fasano, A. Bakker and W.R. Penney, "Advanced Impeller Geometry Boosts. Liquid Agitation”, Chem. Eng., pp. 110-116, Aug. 1994.
[17] M. Blažej, M. Juraščík, J. Annus and J. Markoš, "Measurement of mass transfer coefficient in an airlift reactor with internal loop using coalescent and non-coalescent liquid media”, J. Chem. Technol. Biotechn., vol. 29, no. 12, pp. 1405-1411, Dec. 2004.
[18] M.O. Cerri and A.C. Badino, "Oxygen transfer in three scales of concentric tube airlift bioreactors”, Biochem. Eng. J., vol. 51, pp. 40-47, Aug. 2010.
[19] Y. Kawase and N. Hashimoto, "Gas hold-up and oxygen transfer in three-phase external-loop airlift bioreactors: Non-Newtonian
[20] J. Kulkarni, "Computational modeling of industrial biofuel reactors”, in International ANSYS conference 2008.
[21] Y.T. Shah, B.G. Kelkar, S.P. Godbole and W.D. Deckwer, "Design parameters estimations for bubble column reactors”, AICHE J. vol. 28, pp. 353-379, May 1982.