Leaf Pigments Help Almond Explants Tolerating Osmotic Stress

Authors: Soheil Karimi, Abbas Yadollahi, Kazem Arzani, Ali Imani

Abstract:

This study was conducted to evaluate the response of almond genotypes to osmotic stress in vitro in order to screen drought tolerance. Explants subjected to polyethyleneglycol osmotic stress (0, 3.5, and 7.0% WV) on the MS medium. Concentrations of photosynthesis pigments, anthocyanins, and carothenoids were significantly reduced under osmotic stress. Under osmotic stress, leaf water content, cellular membrane stability and pigments concentrations were significantly higher in the leaves of drought tolerant genotypes. The results revealed that carotenoids and anthocyanins may act as photoprotectant compounds in almond leaves and involved in drought tolerance system of the plant.

Keywords: Almond, Anthocianins, Carotenoids, in vitro; Leaf Osmotic Stress, Leaf Pigments, Polyethylene Glycol.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1326810

References:

[1] B. Shiran, N. Amirbakhtiar, S. Kiani, Sh. Mohammadi, B. E. Sayed-Tabatabaei, and H. Moradi, "Molecular characterization and genetic relationship among almond cultivars assessed by RAPD and SSR markers,” Sci. Hort., vol. 111, pp. 280–292, Feb. 2007.
[2] J. Gomes-Laranjo, J. P. Courtinho, V. Galhani, and V. Cordovo, "Responses of five almond cultivars to irrigation: Photosynthesis and leaf water potential,” Agric. Water Manag., vol. 83, pp. 261-265, Jan. 2006.
[3] A. Isaakidis, T. Sotirpoulos, D. Almaliotis, I. Therois, and D. Styliandis, "Response to severe water stress if almond Prunuse amygdalus Ferragnes grafted on eight rootstocks,” New Zeal. J. Crop Hort. Sci., vol. 32, pp. 355-362, Mar. 2004.
[4] A. Yadollahi, K. Arzani, A. Ebadi, M. Wirthensohn, and S. Karimi, "The response of different almond genotypes to moderate and severe water stress in order to screen for drought tolerance,” Sci. Hort., 129: 403–413, June 2011.
[5] S. Karimi, S. Hojati, S. Eshghi, R. Nazary Moghaddam, and S. Jandoust, "Magnetic exposure improves tolerance of fig ‘Sabz’ explants to drought stress induced in vitro,” Sci. Hort., vol. 137, pp. 95–99, Jan. 2012.
[6] F. Larhel, L. Leport, M. Petrivalsky, and M. Chappart, "Effectors for the osmoinduced proline response in higher plants,” Plant Physiol. Biochem., vol. 31, pp. 911-922. 1993.
[7] J.W. Heyser, and M.W. Nabors, "Growth, water content and solute accumulation of two tobacco cell lines cultured on sodium chloride, dextran and polyethylene glycol,” Plant Physiol., vol. 68, pp. 1454–1459. Dec. 1979.
[8] K.M. Fallon, and R. Phillips, "Responses to water stress in adapted and unadapted carrot cell suspension cultures,” J. Exp. Bot., vol. 40, pp. 681-687, Sep.1989.
[9] R. Dragiiska, D. Djilianov, P. Denchev, and A. Atanassov, "In vitro selection for osmotic tolerance in alfalfa (Medicago sativa L.),” Bulg. J. Plant Physiol., Vol. 22, pp. 30–39, Nov. 1996.
[10] M.A. Aazami, M. Torabi, and E. Jalili, "In vitro response of promising tomato genotypes for tolerance to osmotic stress,” Afr. J. Biotech., vol. 9, pp. 4014-4017, Jun. 2010.
[11] H. Turhan, and I. Baser, "In vitro and in vivo water stress in sunflower (Helianthus annuus L.),” Helia. vol .27, pp. 227-236, Jan. 2004.
[12] S.H., Wani, P.A. Sofi, S.S. Gosal,, and N.B. Singh, "In vitro screening of rice (Oryza sativa L) callus for drought tolerance,” Commu. Biom. Crop Sci., vol. 5, pp. 108–115, Dec. 2010.
[13] M.D. Rumbaugh, and D.A. Johnson, "Screening alfalfa germplasm for seedling drought resistance,” Crop Sci., vol. 21, pp. 704-713, Sept. 1981.
[14] L.M. Kent, and A. Lauchli., "Germination and seedling growth of cotton: salinity-calcium interaction,” Plant Cell Environ., vol. 8, pp. 155-159, 1985.
[15] A. Blum, and E. Ebercon, "Cell membrane stability as a measure of drought and heat tolerance in wheat,” Crop Sci., vol. 21, pp. 43-47, Feb. 1981.
[16] A.R.; Wellburn, "The spectral determination of chlorophyll a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution,” J. Plant Physiol., vol. 144, pp. 307-313, Apr. 1994.
[17] S. O. Neill, "The functional role of anthocyanins in leaves”. Ph.D. dissertation, School of Biological Sciences. University of Auckland, Auckland, New Zealand. Sep. 2002.
[18] N. Sivritepe, U.; Erturk, C.; Yerlikaya, and I.; Turkan, "Response of the cherry rootstock to water stress induced in vitro,” Biol. Planta., vol. 523, pp. 573-576, Nov. 2008.
[19] Bajji, M., J.M. Kinet, and S. Lutts, "The use of the electrolyte leakage method for assessing cell membrane stability as a water stress tolerance test in durum wheat,” Plant Growth Regul., vol. 36, pp. 61-70, Jan. 2001.
[20] A.R.; Wellburn, "The spectral determination of chlorophyll a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution,” J. Plant Physiol., vol. 144, pp. 307-313, 1994.
[21] R.K. Sairam, D.S. Shukla, and D.C. Saxena, "Stress induced injury and antioxidant enzymes in relation to drought tolerance in wheat genotypes,” Biol. Planta., vol. 40, pp. 357-364. Jan.1998.
[22] M. E. Griffiths, R. P. Keith, and C. M. Orians, "Direct and indirect effects of salt spray and fire on coastal heath and plant physiology and community composition,” Rhodora, vol. 108, pp. 32-42, 2006.
[23] B.; Loggini, A. Scartazza, and F. Navari-Izzo, "Antioxidative defense system, pigment composition, and photosynthetic efficiency in two wheat cultivars subjected to drought,” Plant Physiol., vol. 119, pp. 1091-1099, Mar. 1999.
[24] S. A. Anjum, L. C. Wang, M. Farooq, and C. M. Zou, "Brassinolide application improves the drought tolerance in maize through modulation of enzymatic antioxidants and leaf gas exchange,” J. Agron. Crop Sci., vol. 197, pp. 177-185, Jan. 2011.
[25] D.C. Close, C.L. Beadle, G.K. Holz, and P.H. Brown, "Effect of shadecloth tree shelters on cold-induced photoinhibition, foliar anthocyanin and growth of Eucalyptus globules and E. nitens seedlings during establishment,” Austral. J. Bot., vol. 50, pp. 15–20, 2002.