Molecular Detection and Characterization of Infectious Bronchitis Virus from Libya
Infectious bronchitis virus (IBV) is a very dynamic and evolving virus, causing major economic losses to the global poultry industry. Recently, the Libyan poultry industry faced severe outbreak of respiratory distress associated with high mortality and dramatic drop in egg production. Tracheal and cloacal swabs were analyzed for several poultry viruses. IBV was detected using SYBR Green I real-time PCR detection based on the nucleocapsid (N) gene. Sequence analysis of the partial N gene indicated high similarity (~ 94%) to IBV strain 3382/06 that was isolated from Taiwan. Even though the IBV strain 3382/06 is more similar to that of the Mass type H120, the isolate has been implicated associated with intertypic recombinant of 3 putative parental IBV strains namely H120, Taiwan strain 1171/92 and China strain CK/CH/LDL/97I. Complete sequencing and antigenicity studies of the Libya IBV strains are currently underway to determine the evolution of the virus and its importance in vaccine induced immunity. In this paper we documented for the first time the presence of possibly variant IBV strain from Libya which required dramatic change in vaccination program.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1336086Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2162
 Cavanagh D. 2007.Coronavirus avian infectious bronchitis virus. Veterinary Research 38(2):281-297.
 Cavanagh, D., and S. A. Naqi. 2003. Infectious bronchitis. Pages 101–119 in Diseases of Poultry. A. M. Saif, Y. M. Fadly, L. R. McDougald, and D. E. Swayne, ed. Iowa State University Press, Ames.
 Ignjatovic J, Sapats S. Identification of previously unknown antigenic epitopes on the S and N proteins of avian infectious bronchitis virus. Archives of Virology 2005; 150(9):1813-1831.
 Chen H, Gill A, Dove BK, Emmett SR, Kemp CF, Ritchie MA, Dee M, Hiscox JA. Mass spectroscopic characterization of the coronavirus infectious bronchitis virus nucleoprotein and elucidation of the role of phosphorylation in RNA binding by using surface plasmon resonance. Journal of Virology 2005; 79(2):1164-1179.
 Hager Bourogâa, Khaled Miled, LatifaGribâa, Imen El Behi, and Abdeljelil Ghram. 2009. Characterization of new variants of avian infectious bronchitis virus in Tunisia. Avian Diseases, 53(3): 426-433.
 Abdel-Moneim AS, Afifi MA, El-Kady MF. 2012. Emergence of a novel genotype of avian infectious bronchitis virus in Egypt. Archives of Virology, 157(12):2453-7.
 Chen H. W., Huang Y. P., and Wang C. H. Identification of intertypic recombinant infectious bronchitis viruses from slaughtered chickens. 2010 Poultry Science 89 :439–446.
 Jinling Feng, Yanxin Hu, Zhijun Ma, Qi Yu, Jixun Zhao, Xiaodong Liu, and Guozhong Zhang. Virulent Avian Infectious Bronchitis Virus, People’s Republic of China. Emerging Infectious Diseases • www.cdc.gov/eid • Vol. 18, No. 12, December 2012.
 Mustafa Ababneh, Abd Elhafeed Dalab, Saad Alsaad, and Mohammad Al-Zghoul Presence of Infectious Bronchitis Virus Strain CK/CH/LDL/97I in the Middle East. ISRN Veterinary Science Volume 2012, Article ID 201721, 6 pages doi:10.5402/2012/201721.
 Zulperi Z, Omar AR and Arshad SS. 2009. Sequence and phylogenetic analysis of S1, S2, M and N genes of infectious bronchitis virus isolates from Malaysia. Virus Genes, 38:383-391.